Article
Pharmacology & Pharmacy
Kirstin Meiners, Prisca Hamm, Marcus Gutmann, Jan Niedens, Agnieszka Nowak-Krol, Salvador Pane, Tessa Luehmann
Summary: The study developed three polyethylene glycol (PEG)ylated tPA bioconjugates based on the recombinant human tPA drug Alteplase using site-specific conjugation strategies. These bioconjugates showed improved proteolytic activity, slower inhibition kinetics by PAI-1, and increased hydrodynamic size.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Obstetrics & Gynecology
Yukiko Asano, Takayuki Iwaki, Kazuo Umemura, Naohiro Kanayama, Hiroaki Itoh
Summary: The study showed that fibrin had no effect on mouse blastocysts, but significantly suppressed trophoblast growth in human blastocysts. However, trophoblasts in human blastocysts then switched to growth promotion through increased fibrinolysis with urokinase-type plasminogen activator (uPA) activity.
HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Hadj Ahmed. Mostefai, Kim M. Baeten, Andrew J. Lucking, David E. Newby, Nuala A. Booth, Nicola J. Mutch
Summary: The resolution of arterial thrombi relies on the endogenous fibrinolytic system. tPA primarily localizes to the thrombus head in a free and active form. Thrombi formed under high shear conditions are more resistant to fibrinolysis due to less tPA and increased PAI-1 concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Guntur Fibriansah, Elisa X. Y. Lim, Jan K. Marzinek, Thiam-Seng Ng, Joanne L. Tan, Roland G. Huber, Xin-Ni Lim, Valerie S. Y. Chew, Victor A. Kostyuchenko, Jian Shi, Ganesh S. Anand, Peter J. Bond, James E. Crowe, Shee-Mei Lok
Summary: Dengue virus has four serotypes (DENV1-4) with different strains within each serotype. The virus can exhibit smooth or bumpy surface morphologies at 37 degrees C, depending on the strain. High-affinity therapeutic antibodies are able to neutralize diverse morphologies of dengue virus.
Article
Hematology
Tae Ito, Yuko Suzuki, Hideto Sano, Naoki Honkura, Francis J. Castellino, Tetsumei Urano
Summary: The study demonstrates the existence of three distinct forms of high-molecular-weight complexes between tPA and PAI-1. Peptide sequence analysis revealed that these complexes consist of nonacylated-enzyme inhibitor complexes, with only a fraction of the population releasing free tPA under physiological conditions.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Medicine, Research & Experimental
Florence Boissiere-Michot, Caroline Mollevi, Volker Baecker, Evelyne Crapez, William Jacot
Summary: Urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) have been identified as prognostic and predictive biomarkers in breast cancer, but their clinical use is limited due to the need for fresh-frozen or fresh tumor samples for quantification. This study compared the expression levels of PLAU and SERPINE1 mRNA (corresponding to uPA and PAI-1 proteins) using in situ hybridization with immunometric assay in breast tumor samples. The results showed that in situ hybridization cannot replace the immunometric assay for quantification of uPA and PAI-1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Medicine, General & Internal
Esra'a Abudouleh, Fatimah Alhamlan, Arwa A. Al-Qahtani, Marie Fe Bohol, Amal Al Hazzani, Khadija Khorfan, Morad Alkaff, Tarek Owaidah, Ahmed A. Al-Qahtani
Summary: This study investigated coagulation and fibrinolysis parameters in COVID-19 patients and their association with disease severity. The results showed significantly elevated levels of thrombin activatable fibrinolysis inhibitor, fibrinogen, and tissue plasminogen activator in severe patients. Additionally, patients in the ICU had significantly higher levels of plasminogen activator inhibitor-1, tissue plasminogen activator, and thrombin activatable fibrinolysis inhibitor. These coagulation markers can serve as suitable prognostic indicators for hypofibrinolysis.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Maria D. Valls, Maria Soldado, Jorge Arasa, Miguel Perez-Aso, Adrienne J. Williams, Bruce N. Cronstein, M. Antonia Noguera, M. Carmen Terencio, M. Carmen Montesinos
Summary: Activation of adenosine A(2A) receptor can promote angiogenesis by increasing the expression of annexin A2 in human dermal microvascular endothelial cells, which enhances fibrinolysis and contributes to wound healing and tissue revascularization.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
David Karasek, Jaromira Spurna, Dominika Macakova, Ondrej Krystynik, Veronika Kucerova
Summary: This study compared vascular damage indicators in different age groups of patients with type 2 diabetes (T2D) and determined their relationship to glycometabolic control. The results showed that older patients had higher arterial stiffness parameters, while markers of endothelial damage were unrelated to glycometabolic control. Age and systolic blood pressure were major determinants of arterial stiffness in T2D patients.
Article
Biochemistry & Molecular Biology
Kaito Harada, Takashi Yahata, Makoto Onizuka, Abd Aziz Ibrahim, Eri Kikkawa, Toshio Miyata, Kiyoshi Ando
Summary: The study highlights the inhibitory role of plasminogen activator inhibitor type-1 (PAI-1) in hematopoietic regeneration in bone marrow microenvironments with abundant bone marrow adipocytes (BMAs), suggesting that blocking PAI-1 activity could be a novel therapeutic approach. PAI-1 inhibitor treatment significantly increases stem cell factor concentration in obese patients, contributing to improved hematopoietic regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Obstetrics & Gynecology
Esra Demirel, Reem Sabouni, Neelima Chandra, Ov D. Slayden, David F. Archer
Summary: The study investigates the distribution and concentrations of several receptors and activators within the primate endometrium during artificial menstrual cycles, showing similarities in their expression patterns suggesting overlapping pathways. These findings indicate potential interactions between the plasminogen activator system and steroid receptors in the endometrium.
REPRODUCTIVE SCIENCES
(2022)
Article
Urology & Nephrology
Victor M. Schuettfort, Benjamin Pradere, David D'Andrea, Nico C. Grossmann, Fahad Quhal, Hadi Mostafaei, Ekaterina Laukhtina, Keiichiro Mori, Michael Rink, Pierre I. Karakiewicz, Reza Sari Motlagh, Satoshi Katayama, Yair Lotan, Douglas Scherr, Mohammad Abufaraj, Harun Fajkovica, Eva Comperat, Dmitry Enikeev, Shahrokh F. Shariat
Summary: Elevated preoperative plasma levels of uPA, SuPAR, and PAI-one were confirmed to be associated with aggressive disease features and worse survival outcomes in patients treated with RC for UCB. These biomarkers have the potential to identify patients who may benefit from intensified/multimodal therapy and improve the discriminatory power of predictive/prognostic models.
JOURNAL OF UROLOGY
(2021)
Article
Immunology
Te-Sheng Lien, Der-Shan Sun, Shih-Che Hung, Wen-Sheng Wu, Hsin-Hou Chang
Summary: The study shows that the dengue virus envelope protein domain III can induce neutrophil NETosis processes, and inhibition of Nlrp3 inflammasome can suppress this process, providing a new molecular target for treating dengue-induced inflammatory pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Health Care Sciences & Services
Hee Young Cho, Han Sung Park, Eun Hee Ahn, Eun Ju Ko, Hyeon Woo Park, Young Ran Kim, Ji Hyang Kim, Woo Sik Lee, Nam Keun Kim
Summary: The study revealed that PAI-1 rs1050955 and REN 10795 rs5707 alleles may decrease the risk of RPL, while the tPA rs4646972 genotype may increase the risk of RPL.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biology
Matthieu Lepine, Sara Douceau, Gabrielle Devienne, Paul Prunotto, Sophie Lenoir, Caroline Regnauld, Elsa Pouettre, Juliette Piquet, Laurent Lebouvier, Yannick Hommet, Eric Maubert, Veronique Agin, Bertrand Lambolez, Bruno Cauli, Carine Ali, Denis Vivien
Summary: The study reveals that tPA released from cortical FS-PV interneurons in the central nervous system can reduce the density of PNNs by degrading CSPGs. This finding provides valuable insights into the control of brain plasticity.
Article
Pharmacology & Pharmacy
Yen-Chung Lai, Yung-Chun Chuang, Chih-Peng Chang, Yee-Shin Lin, Guey-Chuen Perng, Han-Chung Wu, Shie-Liang Hsieh, Trai-Ming Yeh
ANTIVIRAL RESEARCH
(2018)
Article
Infectious Diseases
Liang-Hsuan Lu, Chiao-Hsuan Chao, Trai-Ming Yeh
JOURNAL OF INFECTION
(2019)
Article
Microbiology
Chiao-Hsuan Chao, Wei-Chueh Wu, Yen-Chung Lai, Pei-Jane Tsai, Guey-Chuen Perng, Yee-Shin Lin, Trai-Ming Yeh
Article
Immunology
Yu-San Kao, Chia-Yi Yu, Hong-Jyun Huang, Sen-Mao Tien, Wan-Yu Wang, Martyr Yang, Robert Anderson, Trai-Ming Yeh, Yee-Shin Lin, Shu-Wen Wan
JOURNAL OF IMMUNOLOGY
(2019)
Article
Medicine, Research & Experimental
Ying-Ray Lee, Shan-Ying Wu, Ruei-Yi Chen, Yee-Shin Lin, Trai-Ming Yeh, Hsiao-Sheng Liu
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
(2020)
Review
Microbiology
Yen-Chung Lai, Chiao-Hsuan Chao, Trai-Ming Yeh
Article
Biochemistry & Molecular Biology
Zi-Yi Lu, Miao-Huei Cheng, Chia-Yi Yu, Yee-Shin Lin, Trai-Ming Yeh, Chia-Ling Chen, Chien-Chin Chen, Shu-Wen Wan, Chih-Peng Chang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Zi-Miao Liu, Shu-Jem Su, Yin-Chun Tien
Summary: This study compared stratified chondrocyte sheets (S-CS) and heterogeneous chondrocyte sheets (H-CS) in terms of expression and therapeutic effects, revealing that the S-CS group exhibited higher cell viability, proliferation rate, expression of chondrogenic markers, secretion of tissue inhibitor of metalloproteinase, and GAG production level. The mini-pigs articular cartilage defect model showed that the S-CS group had a higher International Cartilage Repair Society (ICRS) macroscopic score and displayed a zonal structure closer to native cartilage.
Article
Immunology
Hong-Jyun Huang, Martyr Yang, Hsin-Wei Chen, Shuying Wang, Chih-Peng Chang, Tzong-Shiann Ho, Yu-San Kao, Sen-Mao Tien, Hsing-Han Lin, Po-Chun Chang, Yen-Chung Lai, Yu-Peng Hsiao, Yi-Ling Liu, Chiao-Hsuan Chao, Robert Anderson, Trai-Ming Yeh, Yee-Shin Lin, Shu-Wen Wan
Summary: This study developed a modified DENV vaccine candidate with enhanced features by combining modifications of two DENV proteins. The vaccine candidate showed protective effects against DENV infection in a mouse model, indicating its potential clinical application.
Article
Microbiology
Sen-Mao Tien, Po-Chun Chang, Yen-Chung Lai, Yung-Chun Chuang, Chin-Kai Tseng, Yu-San Kao, Hong-Jyun Huang, Yu-Peng Hsiao, Yi-Ling Liu, Hsing-Han Lin, Chien-Chou Chu, Miao-Huei Cheng, Tzong-Shiann Ho, Chih-Peng Chang, Shu-Fen Ko, Che-Piao Shen, Robert Anderson, Yee-Shin Lin, Shu-Wen Wan, Trai-Ming Yeh
Summary: Dengue virus (DENV) is a global health problem without effective treatment. This study demonstrates the potential of humanized anti-NS1 monoclonal antibodies (mAbs) as a therapeutic tool for DENV infection. The humanized mAbs showed therapeutic benefits in reducing bleeding time and skin hemorrhage, and they also inhibited vascular permeability and enhanced cytolysis of DENV-infected cells.
Article
Immunology
Yen-Chung Lai, Yu-Wei Cheng, Chiao-Hsuan Chao, Yu-Ying Chang, Chi-De Chen, Wei-Jiun Tsai, Shuying Wang, Yee-Shin Lin, Chih-Peng Chang, Woei-Jer Chuang, Li-Yin Chen, Ying-Ren Wang, Sui-Yuan Chang, Wenya Huang, Jen-Ren Wang, Chin-Kai Tseng, Chun-Kuang Lin, Yung-Chun Chuang, Trai-Ming Yeh
Summary: In COVID-19 patients, elevated levels of ACE2 antibodies were found to cross-react with S1-RBD antibodies, suggesting a potential mechanism of immune response induced by SARS-CoV-2 infection. This cross-reactivity highlights the complex interactions between the virus and the host immune system in COVID-19 pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Yi-Ling Cheng, Chiao-Hsuan Chao, Yen-Chung Lai, Kun-Han Hsieh, Jen-Ren Wang, Shu-Wen Wan, Hong-Jyun Huang, Yung-Chun Chuang, Woei-Jer Chuang, Trai-Ming Yeh
Summary: This study confirms the presence of antibodies related to SARS-CoV-2 in dengue virus patients, and these antibodies can cross-react with proteins of the dengue virus. These antibodies can inhibit the pathological reactions caused by dengue virus infection, potentially providing some level of protection against dengue virus.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Chiao-Hsuan Chao, Dayna Cheng, Sheng-Wen Huang, Yung-Chun Chuang, Trai-Ming Yeh, Jen-Ren Wang
Summary: This study collected serum samples from 113 Taiwan participants after receiving different SARS-CoV-2 vaccines and compared the differences in immune responses. The results showed that mRNA vaccines elicited higher levels of antibodies and had higher broadly neutralizing ability compared to AZD1222. Additionally, the study found a significant increase in anti-ACE2 autoantibodies after vaccination.
FRONTIERS IN IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
T. M. Yeh
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Review
Cell Biology
Hong-Ru Chen, Yen-Chung Lai, Trai-Ming Yeh
JOURNAL OF BIOMEDICAL SCIENCE
(2018)