Journal
MULTIDISCIPLINARY RESPIRATORY MEDICINE
Volume 10, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s40248-015-0034-z
Keywords
Apoptosis; Micronucleus; Necrosis; Pulmonary diseases
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Funding
- Fundacao de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS) from Brazil
- Departamento de Ciencia e Tecnologia da Secretaria de Ciencia, Tecnologia e Insumos Estrategicos do Ministerio da Saude (Decit/SCTIE/MS) through Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) from Brazil
- Secretaria Estadual da Saude do Rio Grande do Sul (SES-RS) through Escola de Saude Publica do Rio Grande do Sul, Hospital Santa Cruz, Projeto de Pesquisa Dano, Reparacao e Susceptibilidade em Doencas Pulmonares from Brazil
- Laboratory of Genetics and Biotechnology - UNISC from Brazil
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Background: Tuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potential target for therapeutic intervention. The aim of this study was to evaluate DNA damage and the frequency of cellular abnormalities in TB, LC and COPD patients by comparing them to healthy subjects. Methods: The detection of DNA damage by a buccal micronucleus cytome assay was investigated in patients with COPD (n = 28), LC (n = 18) and TB (n = 22) and compared to control individuals (n = 17). Results: The COPD group had a higher frequency of apoptotic cells compared to TB and LC group. The TB group showed a higher frequency of DNA damage, defect in cytokinesis, apoptotic and necrotic cells. Patients with LC had low frequency of chromosomal aberrations than TB and COPD patients. Conclusion: COPD patients showed cellular abnormalities that corresponded to cell death by apoptosis and necrosis, while patients with TB presented defects in cytokinesis and dysfunctions in DNA repair that resulted in the formation of micronucleus (MN) besides apoptotic and necrotic cells. Patients with COPD, TB and LC had a low frequency of permanent DNA damage.
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