Article
Infectious Diseases
Gianluca Morroni, Lucia Brescini, Alberto Antonelli, Vincenzo Di Pilato, Sefora Castelletti, Andrea Brenciani, Gloria D'Achille, Marina Mingoia, Eleonora Giovanetti, Simona Fioriti, Annamaria Masucci, Tommaso Giani, Andrea Giacometti, Gian Maria Rossolini, Oscar Cirioni
Summary: A surveillance study conducted in a regional hospital in Italy revealed the presence of Ceftolozane/tazobactam-resistant P. aeruginosa isolates carrying multiple resistance mechanisms acquired by various high-risk clones.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Pablo A. Fraile-Ribot, Javier Fernandez, Maria A. Gomis-Font, Lorena Forcelledo, Xavier Mulet, Carla Lopez-Causape, Antonio Oliver
Summary: The study investigated the mechanisms behind the in vivo switch in resistance phenotype of P. aeruginosa after ceftazidime-avibactam treatment. It was found that a single SNP difference can lead to a change in resistance profile, reverting a carbapenemase phenotype back into an ESBL phenotype. Through genetic analysis, it was discovered that certain mutations can alter the resistance patterns of bacteria in vivo.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Dhammika Leshan, Aye Mya Sithu Shein, Cameron Hurst, Peter N. Monk, Parichart Hongsing, Phatthranit Phattharapornjaroen, William Graham Fox Ditcham, Puey Ounjai, Thammakorn Saethang, Naphat Chantaravisoot, Pattama Wapeesittipan, Sirirat Luk-in, Sasipen Sae-Joo, Sumanee Nilgate, Ubolrat Rirerm, Chanikan Tanasatitchai, Naris Kueakulpattana, Matchima Laowansiri, Tingting Liao, Rosalyn Kupwiwat, Rojrit Rojanathanes, Natharin Ngamwongsatit, Somkanya Tungsanga, Asada Leelahavanichkul, Naveen Kumar Devanga Ragupathi, Vishnu Nayak Badavath, S. M. Ali Hosseini, Talerngsak Kanjanabuch, Nattiya Hirankarn, Robin James Storer, Longzhu Cui, Mohan Amarasiri, Hitoshi Ishikawa, Paul G. Higgins, Stephen M. Stick, Anthony Kicic, Tanittha Chatsuwan, Shuichi Abe
Summary: The novel aztreonam-Ca-EDTA or ceftazidime-avibactam-Ca-EDTA combinations show favorable efficacy and safety in treating carbapenemase-positive Klebsiella pneumoniae (CPKp) infections.
Article
Microbiology
Laurent Poirel, Jose-Manuel Ortiz de la Rosa, Mustafa Sadek, Patrice Nordmann
Summary: The study evaluated the susceptibility of broad-spectrum beta-lactamases to various antibiotics, and found that clavulanic-acid-inhibited extended-spectrum beta-lactamases significantly affected the susceptibility to ceftazidime-avibactam, ceftolozane-tazobactam, and cefiderocol.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Microbiology
Wendy Kloezen, Ria J. Melchers, Panagiota-Christina Georgiou, Johan W. Mouton, Joseph Meletiadis
Summary: The combination of taniborbactam and cefepime showed potent activity against ESBL-producing strains, restoring susceptibility in all Enterobacterales and two-thirds of P. aeruginosa isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Olga Lomovskaya, Debora Rubio-Aparicio, Kirk Nelson, Dongxu Sun, Ruslan Tsivkovski, Mariana Castanheira, Jill Lindley, Jeffery Loutit, Michael Dudley
Summary: QPX7728 is an ultrabroad-spectrum beta-lactamase inhibitor that enhances the potency of multiple beta-lactams against P. aeruginosa, with varied results depending on beta-lactamase production and other intrinsic resistance mechanisms.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.
Article
Microbiology
Nathan B. Pincus, Monica Rosas-Lemus, Samuel W. M. Gatesy, Hanna K. Bertucci, Joseph S. Brunzelle, George Minasov, Ludmilla A. Shuvalova, Marine Lebrun-Corbin, Karla J. F. Satchell, Egon A. Ozer, Alan R. Hauser, Kelly E. R. Bachta
Summary: Resistance to antipseudomonal penicillins and cephalosporins in Pseudomonas aeruginosa is often driven by the overproduction of AmpC beta-lactamase and OXA-10-family beta-lactamases. This study identified a novel allele of bla(OX)(A-10), named bla(OX)(A-935), which confers ceftazidime resistance and showed increased catalytic efficiency for ceftazidime compared to OXA-14. Crystal structures of OXA-14 and OXA-935 revealed that the F153S mutation in OXA-935 increases flexibility in the omega loop. Considering the reliance on novel beta-lactam/beta-lactamase inhibitor combinations, the amino acid changes in OXA-10-family beta-lactamases are of concern.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Infectious Diseases
Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stephane Gerard, Fany Reffuveille
Summary: The combination of non-antimicrobial molecules with antibiotics can significantly reduce bacterial biofilm formation, potentially offering a solution to combat antibiotic resistance emergence.
Article
Pharmacology & Pharmacy
Davoodbasha MubarakAli, Kannappan Arunachalam, Murugan Lakshmanan, Bazigha Badar, Jung-Wan Kim, Sang-Yul Lee
Summary: Biofilm-related nosocomial infections can cause various life-threatening infections. In this study, hydroxyapatite nanoparticles (HANPs) were synthesized using a co-precipitation method followed by microwave treatment, and their ability to inhibit the biofilm formation of Pseudomonas aeruginosa ATCC 10145 was investigated. The results showed that the synthesized HANPs effectively inhibited biofilm formation, suggesting their potential use in controlling biofilm-related infections caused by Pseudomonas aeruginosa.