Article
Agriculture, Dairy & Animal Science
Nicolas Barbeau-Gregoire, Alexandre Boyer, Marjolaine Rousseau, Marie-Lou Gauthier, Jocelyn Dubuc
Summary: The study aimed to validate the accuracy of on-farm bacteriological culture media (Tri-plate and Petrifilm) from endometrial samples compared to the results from the diagnostic laboratory. Results showed that using >90 and >100 colonies as criteria for Tri-plate and Petrifilm on-farm systems respectively gave optimal results when compared to the standard laboratory. This suggests that Tri-plate media with a threshold of >90 colonies is effective in diagnosing endometritis accurately.
Article
Biochemistry & Molecular Biology
Wenxiang Shen, Xiaoyu Ma, Dan Shao, Xiaohu Wu, Shengyi Wang, Juanshan Zheng, Yanan Lv, Xuezhi Ding, Baohua Ma, Zuoting Yan
Summary: The study found that neutrophil extracellular traps (NETs) are involved in bovine endometrial epithelial cell (BEEC) pyroptosis during endometritis in dairy cows.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
A. Husnain, U. Arshad, M. B. Poindexter, R. Zimpel, M. Nehme Marinho, M. C. Perdomo, P. Fan, K. C. Jeong, C. D. Nelson, I. M. Sheldon, J. J. Bromfield, J. E. P. Santos
Summary: The objective of this experiment was to study the effect of inducing endometrial inflammation by infusing utero-pathogenic bacteria on productive performance and reproduction in lactating cows. The results showed that induced endometrial inflammation compromised milk production and reproduction in cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Veterinary Sciences
Bingke Wang, Jinbang Xiao, Yongjie Ma, Chuxi Gao, Hanbing Li, Yonghong Jia, Yaping Jin, Pengfei Lin
Summary: This study aimed to compare the effectiveness of the hormone program M+C with the Pre-OvSynch program. The results showed that the M+C program had significantly better pregnancy rates and reduced the number of artificial inseminations compared to the Pre-OvSynch program, while also significantly reducing open days.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
J. Dubuc, V Fauteux, M. Villettaz-Robichaud, J-P Roy, M. Rousseau, S. Buczinski
Summary: This study aimed to quantify the efficacy of a second intrauterine cephapirin treatment on postpartum dairy cows affected by PVD or ENDO. Results showed that a second treatment increased the pregnancy risk at first insemination in cows affected by PVD and ENDO. Administering a second intrauterine cephapirin infusion 14 days after the initial treatment did improve subsequent reproductive performance in these cows.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Hiroaki Okawa, Missaka M. P. Wijayagunawardane, Peter L. A. M. Vos, Osamu Yamato, Masayasu Taniguchi, Mitsuhiro Takagi
Summary: Intrauterine infusion of chitosan solution in early postpartum dairy cows can accelerate uterine recovery from endometritis, potentially serving as a replacement for prostaglandin F-2 alpha administration. The treatment has antimicrobial effects on the uterus and does not show adverse effects on fertility.
Article
Agriculture, Dairy & Animal Science
Shaked A. Druker, Ron Sicsic, Michael van Straten, Tamir Goshen, Maor Kedmi, Tal Raz
Summary: The study showed that primiparous and multiparous cows should be diagnosed for CEM using different %PMN thresholds at different stages postpartum, providing a comprehensive view of the reproductive prognosis for CEM-positive cows.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Veterinary Sciences
Mojtaba Kafi, Mehran Ghaemi, Mehdi Azari, Abdolah Mirzaei, Samad Azarkaman, Yusof Torfi
Summary: The study showed that cows with subclinical endometritis (SCE) had lower oocyte maturation and fertilization rates compared to normal heifers (NH), with FF from repeat breeder cows resulting in less oocyte maturation and cumulus cell expansion. The lower fertility in repeat breeder cows could be attributed to the lower oocyte maturation rate and lower expression of GDF9, StAR, and FSHr in the cumulus-oocyte complexes.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
Ali Husnain, Usman Arshad, Roney Zimpel, Eduardo Schmitt, Mackenzie J. Dickson, Milerky C. Perdomo, Mariana N. Marinho, Nadia Ashrafi, Stewart F. Graham, Jeanette Bishop, Thomas R. Hansen, Kwang C. Jeong, Angela M. Gonella-Diaza, Ricardo C. Chebel, I. Martin Sheldon, John J. Bromfield, Jose E. P. Santos
Summary: Endometrial inflammation in cows is associated with reduced pregnancy rates and increased pregnancy loss. Induced endometritis alters histotroph composition and induces inflammatory signatures on conceptus, compromising their development. Bacterial-induced endometritis impairs conceptus development by altering histotroph composition and conceptus gene expression in dairy cattle.
BIOLOGY OF REPRODUCTION
(2023)
Article
Veterinary Sciences
Yajuan Li, Xiaoyu Ma, Jie Yang, Xiaohu Wu, Zuoting Yan, Baoxiang He
Summary: During bovine endometritis, the levels of Cathelicidin 1-7 significantly increase, especially in severe cases compared to moderate cases. Bovine endometrial epithelial cells can express Cathelicidin 1-7, and Escherichia coli triggers the release of these proteins. High concentration of E. coli can decrease the mRNA and protein levels of Cathelicidins.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Nyabinwa Pascal, Kashongwe Olivier Basole, Hirwa Claire d'Andre, Bebe Bockline Omedo
Summary: This study identified and quantified the direct and indirect associations of cow- and herd-level risk factors with clinical and subclinical endometritis cases observed among zero-grazed dairy cows. Cow-level risk factors like season of calving, dystocia, and body condition score, along with herd-level factors such as cowshed hygiene and farm size, showed strong direct and indirect influences on the occurrence of endometritis cases. Effective management of these factors should be a priority in extension education to help smallholder farmers prevent and control endometritis in their dairy cows.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Veterinary Sciences
Carolina Menezes Suassuna de Souza, Ewerton de Souza Lima, Raphael Ferreira Ordonho, Bianca Rafaella Rodrigues dos Santos Oliveira, Rebeca Cordeiro Rodrigues, Marquiliano Farias de Moura, Daniel Magalhaes Lima, Maiara Garcia Blagitz, Eduardo Milton Ramos Sanchez, Isac Almeida de Medeiros, Fernando Nogueira Souza, Artur Cezar de Carvalho Fernandes
Summary: This study evaluated the expression of immune checkpoints PD-1 and CTLA-4 in blood T lymphocytes during the periparturient period in dairy cows. The expression of these immune checkpoints was found to be associated with uterine health and immune tolerance. Additionally, the serum acute phase-protein haptoglobin concentration was related to the expression of these immune checkpoints. However, there was no association between the indicators of negative energy balance and the expression of the immune checkpoint markers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Agriculture, Dairy & Animal Science
Ayodele Olaolu Oladejo, Yajuan Li, Xiaohu Wu, Bereket Habte Imam, Jie Yang, Xiaoyu Ma, Zuoting Yan, Shengyi Wang
Summary: This review article provides insights into the molecular pathogenesis of bovine endometritis with a focus on host-pathogen interactions and the potential of nanotechnology-based drug delivery system. Gram-negative bacteria and their ligands have been extensively studied, while research detail on Gram-positive bacteria in bovine endometritis is lacking. Understanding the cellular components and signaling mechanisms in the endometrium is crucial for the development of nanotherapeutic strategies against endometritis.
Article
Agriculture, Dairy & Animal Science
E. T. Kelly, C. G. McAloon, L. O'Grady, M. Duane, J. R. Somers, M. E. Beltman
Summary: Early detection of reproductive tract disease postpartum is crucial for subsequent reproductive outcomes. Methods such as identifying purulent vaginal discharge and endometritis via ultrasound are practical for diagnosis. High PVD or UE scores at prebreeding examination are associated with decreased fertility, with UE scoring of >= 1 having the highest sensitivity and specificity estimates.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Veterinary Sciences
Dario A. Vallejo-Timaran, Ali Bazzazan, Mariela Segura, Nelson E. Prieto-Cardenas, Rejean C. Lefebvre
Summary: The study aimed to describe the local innate immune response during clinical cervicitis in dairy cows. Results showed that at +3 weeks postpartum, IL1, IL8, and AGP concentrations in the uterus and the fornix were significantly higher in CC cows than healthy cows.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Neurosciences
Kenji Nakamura, Yuuki Shiroto, Yuuki Tamura, Keisuke Koyama, Kengo Takeuchi, Makoto Amanuma, Toru Nagasawa, Seiji Ozawa
NEUROSCIENCE LETTERS
(2020)
Article
Agriculture, Dairy & Animal Science
Shogo Higaki, Keisuke Koyama, Yosuke Sasaki, Kodai Abe, Kazuyuki Honkawa, Yoichiro Horii, Tomoya Minamino, Yoko Mikurino, Hironao Okada, Fumikazu Miwakeichi, Hongyu Darhan, Koji Yoshioka
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Yoshimasa Imoto, Tetsuji Takabayashi, Masafumi Sakashita, Yukinori Kato, Kanako Yoshida, Masanori Kidoguchi, Keisuke Koyama, Naoto Adachi, Yukihiro Kimura, Kazuhiro Ogi, Yumi Ito, Masafumi Kanno, Masayuki Okamoto, Norihiko Narita, Shigeharu Fujieda
Letter
Allergy
Aiko Oka, Masanori Kidoguchi, Shin Kariya, Tazuko Fujiwara, Atsushi Yuta, Hiromi Miyashita, Takaya Higaki, Yukiko Ogawa, Kengo Kanai, Sei-ichiro Makihara, Takenori Haruna, Jun Kunisawa, Naoto Adachi, Keisuke Koyama, Rieko Ii, Emiko Noguchi, Shigeharu Fujieda, Kazunori Nishizaki, Mitsuhiro Okano
Letter
Allergy
Masanori Kidoguchi, Wataru Morii, Emiko Noguchi, Atsushi Yuta, Yukiko Ogawa, Takako Nakamura, Hirotaka Kikuoka, Hideaki Kouzaki, Hiroyuki Arai, Rieko Ii, Naoto Adachi, Keisuke Koyama, Takahiro Ninomiya, Yoshimasa Imoto, Masafumi Sakashita, Shigeharu Fujieda
Article
Agriculture, Dairy & Animal Science
Ryo Inaba, Ryouka Kawahara-Miki, Akihisa Shinozawa, Taichi Yasuhara, Takashi Fujii, Keisuke Koyama, Michiko Murata-Okubo, Kousaku Souma, Hiroki Hirayama
Summary: Hormonal induction of parturition in cattle increases the risk of retained fetal membrane. This study revealed the importance of interferon signaling in the immune regulation of placental tissues during parturition.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Pharmacology & Pharmacy
Yoshimasa Imoto, Shigeharu Ueki, Yukinori Kato, Kanako Yoshida, Taiyo Morikawa, Yukihiro Kimura, Masanori Kidoguchi, Toshiki Tsutsumiuchi, Keisuke Koyama, Naoto Adachi, Yumi Ito, Kazuhiro Ogi, Masafumi Sakashita, Takechiyo Yamada, Robert P. Schleimer, Tetsuji Takabayashi, Shigeharu Fujieda
Summary: This study found that serum leptin levels are elevated in ECRS patients and are correlated with the proportion of eosinophils in peripheral blood, the number of eosinophils in nasal polyps, and eotaxin-3 mRNA expression in nasal polyps. Furthermore, the study also found that leptin can enhance eotaxin-3 expression in nasal fibroblasts from nasal polyps in a leptin receptor-dependent manner. These findings suggest a significant role for circulating leptin in the development of eosinophilic inflammation in nasal polyps.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ang Zhang, Keisuke Koyama, Weiwei Wan, Kensuke Harada
Summary: The study introduces a robotic system that manipulates bulky objects by utilizing contact with the environment, effectively combining three manipulation styles: pivoting, tumbling, and regrasping. Through a hierarchical manipulation planner, the system is able to manipulate large and heavy objects while effectively avoiding constraints from arm kinematics.
APPLIED SCIENCES-BASEL
(2021)
Article
Agriculture, Dairy & Animal Science
Keisuke Koyama, Tomoaki Kubo, Takeshi Koyama, Yoshiyuki Takahashi
Summary: This study investigated the relationship between parity and the optimal insemination-ovulation interval (IOI) in lactating dairy cows. It was found that ovulation occurring 6-12 hours after artificial insemination (AI) was associated with the highest conception rate in multiparous cows. However, the effect of IOI on the conception rate in primiparous cows was insignificant. Therefore, careful determination of the timing of AI is essential in multiparous cows.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Thomas Chia-Tang Ho, Noritoshi Kawate, Keisuke Koyama
Summary: This study aimed to establish machine learning-based prediction models for nuclear maturation speed (NMS) of Japanese Black beef heifer oocytes using non-invasive indicators. Morphological features were extracted from recorded photos and used to develop the prediction models. The results showed a correlation between predicted NMS and embryo development, suggesting the potential for improving the efficiency of in vitro embryo production.
Proceedings Paper
Computer Science, Interdisciplinary Applications
Yusuke Hayami, Weiwei Wan, Keisuke Koyama, Peihao Shi, Juan Rojas, Kensuke Harada
Summary: This study introduces a method for predicting error states in robotic snap joint assembly, enabling timely error recovery using functional principal component analysis and support vector machine. Experimental results demonstrate that error states can be accurately predicted and recovered from.
2021 IEEE/SICE INTERNATIONAL SYMPOSIUM ON SYSTEM INTEGRATION (SII)
(2021)
Proceedings Paper
Automation & Control Systems
Satoshi Tanaka, Keisuke Koyama, Taku Senoo, Masatoshi Ishikawa
2020 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND AUTOMATION (ICRA)
(2020)
Article
Agriculture, Dairy & Animal Science
Keisuke Koyama, Takayoshi Takahashi
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2020)
Article
Agriculture, Dairy & Animal Science
Hiroki Hirayama, Ryosuke Sakumoto, Keisuke Koyama, Taichi Yasuhara, Taito Hasegawa, Ryo Inaba, Takashi Fujii, Akira Naito, Satoru Moriyasu, Soichi Kageyama
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2020)
Review
Robotics
Taku Senoo, Yuji Yamakawa, Shouren Huang, Keisuke Koyama, Makoto Shimojo, Yoshihiro Watanabe, Leo Miyashita, Masahiro Hirano, Tomohiro Sueishi, Masatoshi Ishikawa
JOURNAL OF ROBOTICS AND MECHATRONICS
(2019)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.