Article
Biochemistry & Molecular Biology
Zhiqiang Li, Liying Shi, Qianqing Li, Jing Zhao, Wenfa Lu, Jun Wang
Summary: This study investigated the role of circular RNAs in endometritis and identified their potential involvement in various signaling pathways related to disease progression. The findings provide new insights into the potential therapeutic applications of circular RNAs in the treatment of endometritis.
Article
Veterinary Sciences
Elina Tukia, Isa Hallman, Maarit Penttilae, Satu Haenninen, Maria Kareskoski
Summary: Endometritis is a common cause of infertility in mares, often caused by bacteria such as E. coli and beta-haemolytic streptococci. This study aimed to detect the presence and localization of E. coli bacteria in equine endometrial biopsies using a chromogenic RNAscope method. However, no evidence of bacterial invasion was found, regardless of the inflammatory status or previous bacterial culture results. Further research is needed to investigate potential localized infections or biofilm coverings that may have led to undetected bacterial presence.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Yajuan Li, Xiaoyu Ma, Jie Yang, Xiaohu Wu, Zuoting Yan, Baoxiang He
Summary: During bovine endometritis, the levels of Cathelicidin 1-7 significantly increase, especially in severe cases compared to moderate cases. Bovine endometrial epithelial cells can express Cathelicidin 1-7, and Escherichia coli triggers the release of these proteins. High concentration of E. coli can decrease the mRNA and protein levels of Cathelicidins.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Shuang Li, Yiqi Wang, Lianjun Feng, Zhongxian Yu, Min Qiu, Ying Wang, Naisheng Zhang, Xiaoyu Hu, Yunhe Fu
Summary: The study revealed that Bacillus subtilis H28 can alleviate E. coli-induced endometritis symptoms and maintain the endometrial barrier while reducing inflammatory responses.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Wenxiang Shen, Xiaoyu Ma, Dan Shao, Xiaohu Wu, Shengyi Wang, Juanshan Zheng, Yanan Lv, Xuezhi Ding, Baohua Ma, Zuoting Yan
Summary: The study found that neutrophil extracellular traps (NETs) are involved in bovine endometrial epithelial cell (BEEC) pyroptosis during endometritis in dairy cows.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
A. R. Guadagnin, L. K. Fehlberg, B. Thomas, Y. Sugimoto, I. Shinzato, F. C. Cardoso
Summary: This study aimed to investigate the effects of feeding rumen-protected lysine (RPL) prepartum and postpartum on reproductive performance, uterine health, and gene expression. The results showed that feeding RPL prepartum improved uterine immune status, but had no effect on days to first ovulation postpartum.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
X. Zeng, J. Vidlund, B. Gillespie, L. Cao, G. E. Agga, J. Lin, O. Kerro Dego
Summary: Mastitis, a common disease in dairy cows, causes significant economic losses to the industry. Environmental mastitis pathogens pose a major problem for dairy farms. A new vaccine is needed to prevent clinical disease and production losses. A nutritional immunity approach that restricts bacterial iron uptake has been developed, showing promise in controlling mastitis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
A. Husnain, U. Arshad, M. B. Poindexter, R. Zimpel, M. Nehme Marinho, M. C. Perdomo, P. Fan, K. C. Jeong, C. D. Nelson, I. M. Sheldon, J. J. Bromfield, J. E. P. Santos
Summary: The objective of this experiment was to study the effect of inducing endometrial inflammation by infusing utero-pathogenic bacteria on productive performance and reproduction in lactating cows. The results showed that induced endometrial inflammation compromised milk production and reproduction in cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Nicolas Barbeau-Gregoire, Alexandre Boyer, Marjolaine Rousseau, Marie-Lou Gauthier, Jocelyn Dubuc
Summary: The study aimed to validate the accuracy of on-farm bacteriological culture media (Tri-plate and Petrifilm) from endometrial samples compared to the results from the diagnostic laboratory. Results showed that using >90 and >100 colonies as criteria for Tri-plate and Petrifilm on-farm systems respectively gave optimal results when compared to the standard laboratory. This suggests that Tri-plate media with a threshold of >90 colonies is effective in diagnosing endometritis accurately.
Article
Reproductive Biology
Eriky Akio de Oliveira Tongu, Lorenzo G. T. M. Segabinazzi, Marina Landim Alvarenga, Aldine Monteiro, Frederico Ozanam Papa, Marco Antonio Alvarenga
Summary: The study evaluated the effects of mesenchymal stem cell-conditioned medium (MSC-CM) on sperm parameters, intrauterine inflammation, intrauterine fluid accumulation, and fertility in mares. MSC-CM did not affect sperm parameters when mixed with diluted semen, and reduced post-insemination inflammatory responses in mares with different susceptibilities to endometritis. Fertility rates did not differ significantly among groups.
Article
Chemistry, Multidisciplinary
Katarzyna Grudlewska-Buda, Krzysztof Skowron, Ewa Walecka-Zacharska, Natalia Wiktorczyk-Kapischke, Jaroslaw Bystron, Agnieszka Kaczmarek, Eugenia Gospodarek-Komkowska
Summary: The study found that most tested E. coli strains were sensitive to antimicrobials and dipping agents, with a small percentage showing resistance. Majority of the strains did not form biofilm, with strong biofilm formers mainly found in cows with subclinical mastitis.
APPLIED SCIENCES-BASEL
(2021)
Article
Immunology
Mengting Guo, Ya Gao, Yibing Xue, Yuanping Liu, Xiaoyan Zeng, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun, Zhaofei Wang, Yaxian Yan
Summary: The study isolated three lytic phages from sewage of dairy farm, showing broad host range and high efficiency against E. coli. Phage cocktail significantly reduced bacterial number, somatic cells, and inflammatory factors in a mastitis infected cow model, achieving the same effect as antibiotic treatment. Overall, phage cocktail may be a promising alternative therapy against mastitis caused by drug resistant E. coli.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Kristi Jones, Federico Cunha, Soo Jin Jeon, Johanny Perez-Baez, Segundo Casaro, Peixin Fan, Ting Liu, Shinyoung Lee, Kwangcheol Casey N. Jeong, Yongqiang Yang, Klibs N. Galvao
Summary: This study investigated the source and route of bacterial colonization in the uterus of dairy cows. It found that the gastrointestinal tract is likely the source of bacteria that colonize the reproductive tract, ascending through the lower genital tract to reach the uterus. Cow-to-cow transmission was also observed, and strains were not specific to body site or health status.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
J. Dubuc, V Fauteux, M. Villettaz-Robichaud, J-P Roy, M. Rousseau, S. Buczinski
Summary: This study aimed to quantify the efficacy of a second intrauterine cephapirin treatment on postpartum dairy cows affected by PVD or ENDO. Results showed that a second treatment increased the pregnancy risk at first insemination in cows affected by PVD and ENDO. Administering a second intrauterine cephapirin infusion 14 days after the initial treatment did improve subsequent reproductive performance in these cows.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Bingke Wang, Jinbang Xiao, Yongjie Ma, Chuxi Gao, Hanbing Li, Yonghong Jia, Yaping Jin, Pengfei Lin
Summary: This study aimed to compare the effectiveness of the hormone program M+C with the Pre-OvSynch program. The results showed that the M+C program had significantly better pregnancy rates and reduced the number of artificial inseminations compared to the Pre-OvSynch program, while also significantly reducing open days.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Anna J. Korzekwa, Angelika M. Kotlarczyk, Agata A. Szczepanska, Martyna Grzyb, Alicja Siergiej, Izabela Woclawek-Potocka
Article
Biochemistry & Molecular Biology
Magdalena Profaska-Szymik, Anna Galuszka, Anna J. Korzekwa, Anna Hejmej, Ewelina Gorowska-Wojtowicz, Piotr Pawlicki, Malgorzata Kotula-Balak, Kazimierz Tarasiuk, Ryszard Tuz
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agriculture, Dairy & Animal Science
Anna J. Korzekwa, Wladyslaw Kordan, Angelika M. Kotlarczyk, Roland Kozdrowski
Article
Veterinary Sciences
Piotr Andrzej Socha, Barbara Maria Socha
Summary: This paper reports a case of congenital hydrocephalus in a Maine Coon fetus, diagnosed and successfully delivered through ultrasound examination. Ultrasound control and fetal biometry appear to be useful tools for identifying certain pathological conditions in feline pregnancies.
VETERINARNI MEDICINA
(2021)
Article
Multidisciplinary Sciences
Angelika M. Kotlarczyk, Martyna Grzyb, Anna J. Korzekwa
Summary: In the ruminant uterus, steroid production mainly occurs during the reproductive season, especially during the estrous cycle, and continues during anestrus.
SCIENTIFIC REPORTS
(2021)
Review
Agriculture, Dairy & Animal Science
Anna J. Korzekwa, Angelika M. Kotlarczyk
Summary: The use of assisted reproduction techniques in Cervidae is crucial for conservation efforts, particularly in managing endangered species and farmed deer. While some techniques like IVF have shown satisfactory results, others such as cryopreservation still require refinement.
Article
Agriculture, Dairy & Animal Science
Aleksandra Orzolek, Katarzyna T. Rafalska, Wiktoria A. Otowska, Wladyslaw Kordan, Anna J. Korzekwa, Krzysztof Kozlowski
Summary: The addition of zinc nanoparticles to semen extender reduces sperm motility and mitochondrial membrane potential, while maintaining sperm viability. In contrast, adding manganese nanoparticles improves membrane integrity and mitochondrial activity, with the highest SOD activity observed.
Article
Anatomy & Morphology
Jumpei Tomiyasu, Anna Korzekwa, Yusuke K. Kawai, Christian A. Robstad, Frank Rosell, Daisuke Kondoh
Summary: This study investigated the specificities of the beaver vomeronasal system by histologically and immunohistochemically analyzing the VNO and AOB of Eurasian beavers. The results showed that beavers can recognize volatile odorants and nonvolatile substances using the vomeronasal system. The findings highlight the importance of the vomeronasal system in beaver chemical communication.
JOURNAL OF ANATOMY
(2022)
Article
Agriculture, Dairy & Animal Science
Barbara M. Socha, Piotr Socha, Anna Z. Szostek-Mioduchowska, Tomasz Nowak, Dariusz J. Skarzynski
Summary: This study aimed to evaluate and compare the mRNA expression and immunolocalization of PPARα, PPARδ, and PPARγ in the canine endometrium. The results showed that the expression levels of PPARs changed during cystic endometrial hyperplasia (CEH) and CEH-pyometra, suggesting their potential involvement in hormonal disturbances and inflammatory processes.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Barbara Maria Socha, Piotr Lada, Agnieszka Walentyna Jonczyk, Anna Justyna Korzekwa, Dariusz Jan Skarzynski
Summary: The aim of this study was to determine the expression of PPAR isoforms in the bovine corpus luteum throughout the estrous cycle, and their involvement in PGF(2 alpha)-induced processes related to luteolysis. The results showed that the mRNA and protein expression of PPARs changes in the bovine corpus luteum throughout the estrous cycle and under the influence of PGF(2 alpha). PPAR gamma, among all examined isoforms, could be involved in the regulation of PGF(2 alpha)-induced luteolysis in cattle.
Article
Biochemistry & Molecular Biology
Anna Dziekonska, Magdalena Koziorowska-Gilun, Wladyslaw Kordan, Nicoletta M. Neuman, Angelika M. Kotlarczyk, Anna J. Korzekwa
Summary: The aim of this study was to assess the quality and fertilizing potential of red deer epididymal spermatozoa stored in a liquid state for up to 11 days. The results showed a decrease in sperm quality during storage, with differences in fertilizing potential observed between different storage days.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Angelika M. Kotlarczyk, Julia Kaczmarczyk, Olga Witkowska-Pilaszewicz, Malgorzata Kotula-Balak, Anna J. Korzekwa
Summary: This study found that reproductive and condition parameters in seasonally reproducing ruminants such as red deer are dependent on immune status. There is an interaction between immune system activation and AA-metabolite production in the uterus throughout different reproductive stages. IgG, cAMP, haptoglobin, and 6-keto-PGF1 alpha concentrations are valuable markers of reproductive status in hinds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anna J. Korzekwa, Anna Kononiuk, Wladyslaw Kordan, Aleksandra Orzolek
Summary: Metalloproteinases (MMPs) play key roles in various developmental processes and are involved in stem cell biology. This study aimed to investigate the effects of retinoic acid (RA) on MMPs in antler stem cells (ASCs). The results showed that RA increased the expression and output of MMP-3 and TIMP-3 in ASCs, while it had no significant effect on the expression and output of MMP-1 and TIMP-1. The expression profiles of MMPs and TIMPs varied during the differentiation of ASCs into different lineages. These findings provide valuable insights into the physiological and differentiation processes of stem cells.
Meeting Abstract
Agriculture, Dairy & Animal Science
A. Dziekonska, M. Koziorowska-Gilun, W. Kordan, A. Szczepanska, A. Kotlarczyk, A. Korzekwa
REPRODUCTION IN DOMESTIC ANIMALS
(2019)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.