Article
Reproductive Biology
Da-Bin Pyeon, Seung-Eun Lee, Jae-Wook Yoon, Hyo-Jin Park, Seung-Hwan Oh, Do-Geon Lee, Eun-Young Kim, Se-Pill Park
Summary: This study investigated the antioxidant activities of Sasa quelpaertensis Nakai extract (SQE), p-coumaric acid (PCA), and myricetin (MY), and their effects on the in vitro maturation and developmental ability of porcine oocytes. The results showed that SQE and MY have antioxidant effects and enhance in vitro maturation and embryonic development.
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Article
Biochemistry & Molecular Biology
Da-Bin Pyeon, Seung-Eun Lee, Jae-Wook Yoon, Hyo-Jin Park, Chan-Oh Park, So-Hee Kim, Seung-Hwan Oh, Do-Geon Lee, Eun-Young Kim, Se-Pill Park
Summary: The study demonstrates that dieckol, an antioxidant component of Ecklonia cava, can enhance maturation and subsequent development of porcine oocytes in vitro, reducing the negative impact of oxidative stress.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Adel R. Moawad, Ali Salama, Magdy R. Badr, Mohamed Fathi
Summary: Supplementation of L-Carnitine (LC) during in vitro maturation (IVM) of canine oocytes improves maturation, fertilization, and preimplantation embryo development rates following IVF and in vitro culture (IVC).
Article
Agriculture, Dairy & Animal Science
Jose Felipe Warmling Spricigo, Ana Luiza Silva Guimaraes, Andrielle Thainar Mendes Cunha, Ligiane de Oliveira Leme, Marcos Coura Carneiro, Mauricio Machaim Franco, Margot Alves Nunes Dode
Summary: This study aimed to evaluate the potential to predict the quality of oocytes by measuring the transcript abundance of a panel of candidate genes in immature and/or mature cumulus cells. The results showed that none of the selected genes can accurately predict the potential of oocytes to develop into embryos. Additionally, cumulus cell biopsy negatively affects embryo development, blastocyst cell number, and apoptotic cell ratio.
Article
Chemistry, Medicinal
Kangfa Huang, Chengde Li, Fenglei Gao, Yushan Fan, Fanwen Zeng, Li Meng, Li Li, Shouquan Zhang, Hengxi Wei
Summary: The study demonstrated that EGCG promotes the in vitro maturation and embryo development following IVF of porcine oocytes. The protective effects of EGCG on the oocytes may be associated with its antioxidant and anti-apoptosis properties.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara, Takeshige Otoi
Summary: Liposome-mediated gene transfer is a feasible method for establishing a gene targeting framework and producing mutant animals even in laboratories without specialized equipment. However, the efficiency and factors affecting the system in mammalian oocytes and embryos remain unclear.
Article
Environmental Sciences
Zhao-Bo Luo, Mei-Fu Xuan, Sheng-Zhong Han, Zhou-Yan Li, Nasar Khan, Biao-Hu Quan, Xi-Jun Yin, Jin-Dan Kang
Summary: The study demonstrated that ginsenoside Rb1 can protect porcine oocytes from the detrimental effects of methylglyoxal, enhancing maturation rate and improving in vitro developmental capacity, offering novel perspectives for the protection of the reproductive system through the use of ginseng as a functional food.
ENVIRONMENTAL TOXICOLOGY
(2021)
Review
Medicine, General & Internal
Chloe De Roo, Kelly Tilleman
Summary: In vitro maturation of ovarian tissue oocytes is an effective assisted reproductive treatment, but further validation and research are needed for its effectiveness. The introduction of biphasic IVM (CAPA-IVM) has shown significant improvement in the maturation protocol for oocytes.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Sicong Yu, Lepeng Gao, Chang Zhang, Yumeng Wang, Hainan Lan, Qianran Chu, Suo Li, Xin Zheng
Summary: This study demonstrated that glycine can improve ER stress induced by TG treatment and enhance oocyte quality and developmental potential by regulating intracellular calcium levels, mitochondrial function, and ER stress response pathways. Glycine supplementation alleviated ER stress, reduced ROS levels, maintained mitochondrial function, and restored calcium equilibrium in porcine oocytes exposed to ER stress inducers. Glycine also modulated apoptosis-related factors and gene expression, suggesting its potential for enhancing oocyte maturation in vitro.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Jianhua Li, Jing Chen, Tiecheng Sun, Shuiwen Zhang, Tingting Jiao, Ri-Cheng Chian, Youzhu Li, Ye Xu
Summary: In vitro oocyte maturation (IVM) does not affect the quality of embryos and the aneuploidy rate of embryos. The aneuploidy rate of embryos increases with maternal age, especially over 35 years old, whether in IVF or IVM group. Embryos with poor quality, especially grade III embryos, are recommended to be tested by PGS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Obstetrics & Gynecology
A. Christodoulaki, H. He, M. Zhou, A. Cardona Barberan, C. De Roo, S. M. Chuva De Sousa Lopes, M. Baetens, B. Menten, A. Van Soom, P. De Sutter, S. Weyers, A. Boel, D. Stoop, B. Heindryckx
Summary: This study investigates the potential of spindle transfer (ST) to overcome the poor embryo development of in vitro matured ovarian tissue oocytes (OTO-IVM) from testosterone-treated transgender men. The results suggest that ST shows promise in improving the embryo developmental arrest observed in OTO-IVM oocytes.
HUMAN REPRODUCTION
(2023)
Article
Reproductive Biology
Martina Colombo, Isa Mohammed Alkali, Gaia Cecilia Luvoni
Summary: In oocyte cryopreservation programs, vitrification has become the preferred technique due to its feasibility and effectiveness in both veterinary and human medicine. However, vitrification can cause cellular injuries and degeneration of oocytes after warming, leading to low developmental potential. Improvements are needed to enhance the survival and development of vitrified oocytes, including the use of enrichment techniques and regulation of cellular pathways. This review explores the strategies employed so far and discusses future perspectives for cat oocyte vitrification.
Article
Agriculture, Dairy & Animal Science
Pantu Kumar Roy, Ahmad Yar Qamar, Xun Fang, Ghangyong Kim, Seonggyu Bang, Mahanama De Zoysa, Sang Tae Shin, Jongki Cho
Summary: This study demonstrated that treatment with 25 μg/ml CNPs during in vitro maturation can effectively reduce oxidative stress in porcine oocytes, improving their developmental competency.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Cell Biology
Yongzhi Cao, Zhao Wang, Changming Zhang, Yuehong Bian, Xin Zhang, Xin Liu, Wendi Chen, Yueran Zhao
Summary: The addition of metformin to the culture medium of oocytes from aged mice significantly improved oocyte maturation and early embryonic development. Metformin reduced meiotic defects, maintained normal distribution of cortical granules, improved mitochondrial function, reduced apoptosis, increased autophagy, and decreased mitochondrial ROS in oocytes from aged mice. This finding demonstrates the protective effect of metformin on the quality of oocytes from aged mice and provides a potential strategy to improve ART success rates and delay reproductive aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Eunhye Kim, Fang Wu, Xuewen Wu, Hyojung J. Choo
Article
Agriculture, Dairy & Animal Science
Mirae Kim, Seon-Ung Hwang, Junchul David Yoon, Yeon Woo Jeong, Eunhye Kim, Sang-Hwan Hyun
Article
Reproductive Biology
Hyerin Choi, Joohyeong Lee, Junchul David Yoon, Seon-Ung Hwang, Lian Cai, Mirae Kim, Gahye Kim, Dongjin Oh, Eunhye Kim, Sang-Hwan Hyun
Summary: The study showed that supplementation of copper at appropriate concentrations during in vitro maturation improved porcine oocyte maturation and the subsequent embryonic potential of parthenogenetic embryos by reducing oxidative stress and apoptosis.
Article
Biochemistry & Molecular Biology
Seon-Ung Hwang, Kiyoung Eun, Mirae Kim, Junchul David Yoon, Lian Cai, Hyerin Choi, Dongjin Oh, Gabsang Lee, Hyunggee Kim, Eunhye Kim, Sang-Hwan Hyun
Summary: This study demonstrates successful establishment of neuro-organoids derived from porcine embryonic stem-like cells in vitro. The protocol developed in this study can serve as a tool for drug development, patient-specific chemotherapy, and human central nervous system disease studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yongjin Lee, Hanna Lee, Joohyeong Lee, Seung Tae Lee, Geun-Shik Lee, Eunsong Lee
Summary: Glucose supplementation in maturation medium with reduced NaCl concentration significantly improved oocyte maturation and embryonic development in pigs, regardless of the base medium used. Higher blastocyst formation was observed in oocytes matured in medium with 61.6 mM NaCl, indicating the importance of glucose supplementation in enhancing embryonic development through parthenogenesis, somatic cell nuclear transfer, and in vitro fertilization.
Article
Agriculture, Dairy & Animal Science
Dongjin Oh, Joohyeong Lee, Eunhye Kim, Seon-Ung Hwang, Junchul-David Yoon, Lian Cai, Mirae Kim, Gahye Kim, Hyerin Choi, Sang-Hwan Hyun
Summary: The study demonstrates that supplementation with interleukin-7 during porcine in vitro maturation can improve oocyte maturation and developmental potential by upregulating antioxidant-related genes to reduce intracellular oxidative stress. This ultimately enhances the formation of porcine embryos after parthenogenetic activation.
Article
Agriculture, Dairy & Animal Science
Seon-Ung Hwang, Junchul David Yoon, Mirae Kim, Lian Cai, Hyerin Choi, Dongjin Oh, Eunhye Kim, Sang-Hwan Hyun
Summary: The RSPO2 and WNT/CTNNB1 signaling pathways are crucial for porcine follicle development, and are likely involved in the EGFR-ERK signaling pathway.
Article
Agriculture, Dairy & Animal Science
Joohyeong Lee, Eunhye Kim, Seon-Ung Hwang, Lian Cai, Mirae Kim, Hyerin Choi, Dongjin Oh, Eunsong Lee, Sang-Hwan Hyun
Summary: The study showed that treatment with glucuronic acid and N-acetyl-D-glucosamine during porcine oocyte in vitro maturation can enhance oocyte diameter, perivitelline space thickness, reduce reactive oxygen species levels, improve gene expression, and enhance cortical granule reaction. This treatment also led to higher blastocyst rates in both chemically defined and complex media, indicating beneficial effects on early embryonic development after parthenogenesis and somatic cell nuclear transfer.
Article
Cell Biology
Huijeong Ahn, Gilyoung Lee, Jeongeun Kim, Jeongho Park, Seung Goo Kang, Sung-Il Yoon, Eunsong Lee, Geun-Shik Lee
Summary: NLRP3 inflammasome activation inhibits LCN2 secretion while inducing IL-1β in mouse macrophages. The inhibition of NLRP3 triggers on LCN2 secretion is caused by the inhibited transcription and translation of LCN2, suggesting a new linkage between inflammasome activation and LCN2 secretion in the innate immunity.
Article
Veterinary Sciences
Yongjin Lee, Joohyeong Lee, Sang-Hwan Hyun, Geun-Shik Lee, Eunsong Lee
Summary: Using a medium with reduced NaCl enhances cytoplasmic maturation and embryonic development of pig oocytes.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Nuria Marti Gutierrez, Aleksei Mikhalchenko, Hong Ma, Amy Koski, Ying Li, Crystal Van Dyken, Rebecca Tippner-Hedges, David Yoon, Dan Liang, Tomonari Hayama, David Battaglia, Eunju Kang, Yeonmi Lee, Anthony Paul Barnes, Paula Amato, Shoukhrat Mitalipov
Summary: Cells can acquire mitochondrial DNA (mtDNA) from other cells without a parent-offspring relationship, and this acquisition can occur at high levels. The transfer of mtDNA may serve as a compensatory mechanism to restore compromised mitochondrial function. These findings have important implications for understanding mtDNA transfer and developing gene therapies.
Article
Biology
Yeonmi Lee, Aysha Trout, Nuria Marti-Gutierrez, Seoon Kang, Philip Xie, Aleksei Mikhalchenko, Bitnara Kim, Jiwan Choi, Seongjun So, Jongsuk Han, Jing Xu, Amy Koski, Hong Ma, Junchul David Yoon, Crystal Van Dyken, Hayley Darby, Dan Liang, Ying Li, Rebecca Tippner-Hedges, Fuhua Xu, Paula Amato, Gianpiero D. Palermo, Shoukhrat Mitalipov, Eunju Kang
Summary: The research shows that replacing meiotic spindles in mature oocytes with somatic cell nuclei can result in the formation of haploid embryos. Culturing these fertilized embryos can lead to the development of blastocysts, some of which possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs. This finding may provide an alternative strategy for generating oocytes carrying somatic genomes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Minji Kim, Ji-Eun Park, Yongjin Lee, Seung-Tae Lee, Geun-Shik Lee, Sang-Hwan Hyun, Eunsong Lee, Joohyeong Lee
Summary: This study investigated the effect of different growth factors and hormones on the in vitro maturation and developmental competence of oocytes derived from small antral follicles in pigs. It was found that insulin treatment during oocyte growth promotes nuclear maturation, embryonic development, and cumulus cell expansion. Insulin also enhances cytoplasmic maturation, reduces free radical content, and increases maturation-promoting factor activity.
Article
Veterinary Sciences
Ji-Young Jeong, Lian Cai, Mirae Kim, Hyerin Choi, Dongjin Oh, Ali Jawad, Sohee Kim, Haomiao Zheng, Eunsong Lee, Joohyeong Lee, Sang-Hwan Hyun
Summary: This study examined the effects of Ergothioneine (EGT) supplementation during in vitro maturation (IVM) on porcine oocyte maturation and subsequent embryonic development competence after in vitro fertilization (IVF). The results showed that EGT supplementation improved oocyte maturation and embryonic development by reducing oxidative stress in IVM oocytes.
JOURNAL OF VETERINARY SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Haneul Kim, Gon Sup Kim, Sang-Hwan Hyun, Eunhye Kim
Summary: Neuromuscular diseases involve injury or dysfunction of neuromuscular tissue components. In vitro models have been developed to mimic the complex physical and biochemical microenvironments of human neuromuscular tissues, aiding the study and potential therapies for these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.