Article
Biochemistry & Molecular Biology
Tayebeh Dahmardeh, Mohammad Hossein Ghanian, Bita Ebrahimi
Summary: This study evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to alginate hydrogel (ALG). The results showed that HA-SH hydrogel provided better support for follicle growth and had advantages in gene expression levels.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao
Summary: This study achieved low-concentration cryopreservation of preantral follicles by using microencapsulation and nanowarming. The research found that this method improved the viability of preantral follicles and maintained their ability to form cavities, compared to conventional water bath warming. Furthermore, it showed no significant impact on in vitro fertilization and embryo transfer.
NATURE COMMUNICATIONS
(2022)
Article
Reproductive Biology
I. P. Silva, D. C. C. Brito, T. E. S. Silva, R. F. Silva, M. I. F. Guedes, J. Y. G. Silva, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of ZEN and ENL, alone or in combination, on the survival and morphology of in vitro cultured ovarian preantral follicles. The results showed that while ZEN impaired the morphology of follicles, ENL improved the cultured secondary follicles. However, the combination of ENL with ZEN impaired the quality of primary and secondary follicles.
Review
Obstetrics & Gynecology
Lais R. F. M. Paulino, Ernando I. T. de Assis, Venancia A. N. Azevedo, Bianca R. Silva, Ellen da Cunha, Jose R. Silva
Summary: The developmental competence of oocytes is acquired gradually during follicular development, mainly through the accumulation of RNA molecules and proteins. Although there have been attempts to develop in vitro culture systems, achieving oocyte competence in human and domestic animals has not yet been successful. Difficulties in obtaining fertilizable oocytes are related to factors such as the synthesis of numerous mRNAs and proteins, the long duration of follicular development, the size of preovulatory follicles, the composition of the culture medium, and the need for multi-step culture systems. Developing a culture system that maintains bidirectional communication and meets the metabolic demands of different stages of follicle growth is the key to extending the culture period.
REPRODUCTIVE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mengxue Zheng, Jesus Cadenas, Susanne Elisabeth Pors, Tasnim Esa, Stine Gry Kristensen, Linn Salto Mamsen, Cristina Subiran Adrados, Claus Yding Andersen
Summary: This study aimed to optimize the culture conditions for murine preantral follicles to improve their growth and survival. The results showed that altering the alginate concentration, adding estradiol, and increasing follicle-stimulating hormone concentration can promote the growth and development of preantral follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Shizhen Dai, Hanxue Zhang, Feng Yang, Wei Shang, Shenming Zeng
Summary: In this experiment, mouse secondary follicles were cultured in a three-dimensional culture system with different concentrations of IGF-1. The optimal concentration of IGF-1 was determined to be 10 ng/mL based on follicle growth, oocyte maturation, hormone levels, and gene expressions. Moreover, the injection of IGF-1 before superovulation increased the number of ovulated oocytes.
Article
Reproductive Biology
Olena Y. Tkachenko, Shally Wolf, Maralee S. Lawson, Alison Y. Ting, Jhenifer K. Rodrigues, Fuhua Xu, Cecily Bishop, Richard L. Stouffer, Jing Xu
Summary: IGF2, produced by antral follicles in response to steroid hormone regulation, acts as a paracrine factor positively impacting preantral follicle development and function in primates.
BIOLOGY OF REPRODUCTION
(2021)
Article
Veterinary Sciences
Hehe Ren, Yingxin Zhang, Yanping Zhang, Yikai Qiu, Qing Chang, Xiaoli Yu, Xiuying Pei
Summary: This study investigates an optimized three-dimensional (3D) culture system for preantral follicles. The results demonstrate that mature oocytes can be obtained through this FBS-free system, supporting the use of competent oocytes for in vitro fertilization in clinical practice.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Cell Biology
Larissa Zamparone Bergamo, Denis Vinicius Bonato, Camila Bizarro-Silva, Francieli Gesleine Capote Bonato, Tamires Korchovei Sanches, Marcela Bortoletto Cerezetti, Ana Carolina Rossaneis, Waldiceu A. Verri, Fabio Morotti, Marcelo Marcondes Seneda
Summary: This study evaluated the development, morphology, and oxidative stress of preantral ovarian follicles from Bos taurus indicus females grown in vitro with ascorbic acid. The addition of 100 ng/ml ascorbic acid promoted follicular development and maintained morphology and reactive oxygen species stability after 6 days of culture.
Article
Reproductive Biology
Hongyu Liu, Ayman Mesalam, Myeong-Don Joo, Shimin Zhang, Lianguang Xu, Jun Wang, Kyeong-Lim Lee, Seok-Hwan Song, Yu-Guo Yuan, Wenfa Lu, Il-Keun Kong
Summary: The study demonstrated the importance of fibronectin in bovine preantral follicle development and revealed that fibronectin could neutralize the deleterious consequences of kisspeptin, suggesting a potential role in the regulation of the PI3K/Sirt1 signaling pathway in bovine preantral follicle development.
Article
Obstetrics & Gynecology
Renata P. Sousa, Ana Beatriz G. Duarte, Yago Pinto, Naiza A. R. Sa, Benner G. Alves, Francielli W. S. Cibin, Gustavo C. Silva, Camila E. S. Carvalho, Napoleao M. Argolo Neto, Ana Paula R. Rodrigues, Cleidson M. G. Silva, Jose R. Figueiredo, Maria Acelina M. Carvalho
Summary: This study investigated the use of caprine Wharton's Jelly Mesenchymal Stem Cells (WJMSC) on the survival and in vitro development of goat preantral follicles and found that co-culture with stem cells improved activation and early follicular development. The study demonstrates the potential for using MSC in preserving fertility through improving follicular growth.
REPRODUCTIVE SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Livia B. Campos, Andreia M. Silva, Erica C. G. Praxedes, Luana G. P. Bezerra, Jeferson L. S. Freitas, Luciana M. Melo, Alexsandra F. Pereira, Jose R. Figueiredo, Alexandre R. Silva
Summary: The study aimed to investigate the effects of growth differentiation factor 9 (GDF-9) on the in vitro development of ovarian preantral follicles (PAFs) of collared peccaries. The results showed that GDF-9, especially at a concentration of 200 ng/mL in the culture medium, played an active role in the development of PAFs.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Bence Somoskoi, Lilla Bordas, Fusa Uno, Dora Kispal, Linda Muller, Dora Torok, Sandor Cseh
Summary: The present study aimed to compare the effects of different cryopreservation methods (closed vs open vitrification) on the survival and developmental rate of canine isolated preantral follicles (PAFs). The results showed that the OPS method provided superior survival rate, in vitro growth, and hormonal production compared to the CT method. This is the first study to compare cryopreservation protocols for canine isolated preantral follicles.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Article
Cell Biology
Li Ang, Cao Haixia, Li Hongxia, Li Ruijiao, Guo Xingping, Wang Huaixiu
Summary: The results of the study showed that supplementation of CNP during in vitro culture benefited the development of murine preantral follicles, allowing them to reach the antral stage and maintaining good developmental competence of oocytes.
Article
Cell Biology
D. C. C. Brito, L. V. S. Naupas, S. S. Souza, G. L. H. Alcantara, J. R. Figueiredo, R. R. Santos, A. P. R. Rodrigues
Summary: The type and period of fixation affected the percentage of morphologically normal ovarian follicles. Paraformaldehyde was not recommended as a fixative, while formalin was suitable for 12-hour fixation and Carnoy's solution was efficient for 24-hour fixation. Davidson's solution was the most suitable fixative for evaluating the morphology of ovarian preantral follicles, regardless of the fixation period.
Article
Cell Biology
Francisco Denilson Rodrigues Gomes, Danielle Cristina Calado de Brito, Naiza Arcangela Ribeiro de Sa, Lucy Vanessa Sulca Naupas, Gaby Judith Quispe Palomino, Renato Felix da Silva, Everton Pimentel Ferreira Lopes, Gildas Tetaping Mbemya, Benner Geraldo Alves, Mary Zelinski, Jose Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
Summary: The study found that VSF can grow and develop during 12 days of in vitro culture, and showed evidence of preservation of steroidogenic function and remodeling of the basement membrane.
CELL AND TISSUE BANKING
(2022)
Article
Cell Biology
J. Cadenas, N. A. R. Sa, A. C. A. Ferreira, V. M. Paes, F. L. N. Aguiar, C. C. L. Fernandes, R. Rossetto, B. G. Alves, G. A. Apgar, D. M. Magalhaes-Padilha, D. Rondina, J. R. Figueiredo
Summary: This study evaluated the effects of whole flaxseed as a feed supplementation on the in vitro development of caprine early antral follicles and embryo production. The results showed that flaxseed did not affect follicular growth and diameter, but increased the recovery rate of oocytes and negatively affected sperm penetration rate.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Article
Reproductive Biology
A. D. Sales, A. B. G. Duarte, R. M. P. Rocha, I. R. Brito, Y. Locatelli, B. G. Alves, K. A. Alves, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study aimed to investigate the relative abundances of ABC transporters mRNA transcripts in ovine ovarian tissue after vitrification and in vitro culture. The results showed a negative correlation between degenerated follicles and mRNA transcript abundances of ABCB1 and ABCG2, with a significant decrease in the percentage of growing follicles after in vitro culture. Further studies are needed to explore the functions of ABC transporters in ovine folliculogenesis after in vitro culture.
Article
Cell Biology
D. C. C. Brito, I. P. Silva, A. C. A. Ferreira, N. A. R. Sa, M. I. F. Guedes, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of zearalenone and matairesinol on the morphology of in vitro-cultured ovarian follicles. Zearalenone impaired the follicle morphology, while the combination of matairesinol and zearalenone counteracted the negative effects. However, matairesinol did not promote follicle development in vitro.
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Reproductive Biology
A. C. A. Ferreira, N. A. R. Sa, R. F. Silva, F. G. C. Sousa, J. C. Anjos, J. Cadenas, B. G. Alves, L. F. Lima, J. J. H. Celestino, M. O. Gastal, A. P. R. Rodrigues, E. L. Gastal, J. R. Figueiredo
Summary: This study compared the morphological features, estradiol production, and gene expression between in vitro-derived and in vivo-derived early antral caprine follicles. The results showed that in vitro-derived antral follicles cultured in a specific medium had comparable oocyte maturation rates to in vivo-derived early antral follicles.
Article
Veterinary Sciences
R. F. Silva, L. F. Lima, Anna C. A. Ferreira, A. F. B. Silva, D. R. Alves, B. G. Alves, A. C. Oliveira, Selene M. Morais, Ana Paula R. Rodrigues, Regiane R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of different concentrations of eugenol, ascorbic acid, and anethole in goat ovarian tissue. The results showed that eugenol promoted follicle development and survival, and reduced oxidative stress. Furthermore, eugenol decreased gene methylation and endoplasmic reticulum stress.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Fabio R. Vasconcelos, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. The results showed that IVM caused upregulation and downregulation of proteins in COCs. Upregulated proteins in mature COCs after IVM were related to immune response, complement cascade, cell cycle, and extracellular matrix organization, while downregulated proteins in immature COCs after IVM were linked to metabolic processes, immune response, and complement cascade.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Reproductive Biology
A. F. B. Silva, L. F. Lima, A. N. P. Morais, L. L. Lienou, Y. F. Watanabe, D. C. Joaquim, S. M. Morais, D. R. Alves, A. F. Pereira, A. C. Santos, B. G. Alves, D. M. M. Padilha, E. L. Gastal, J. R. Figueiredo
Summary: This study investigates the effects of eugenol supplementation on bovine oocyte in vitro maturation and the subsequent in vitro embryo production and quality through IVF. The results show that adding 40μM eugenol to the maturation medium improves antioxidant capacity and cleavage rate. Additionally, lower calreticulin levels and higher total cell numbers in expanded blastocysts were observed in the lower eugenol concentration treatments. Based on these findings, enriching the maturation medium with 10μM eugenol is recommended for in vitro bovine embryo production.
Article
Agriculture, Dairy & Animal Science
Kamila S. Otavio, Jose R. S. Passos, Renato F. Silva, Laritza F. Lima, Jesus Cadenas, Victor M. Paes, Hudson H. Correia, Anna Clara A. Ferreira, Francisca G. Canafistula, Maria Julia B. Bezerra, Lais L. B. Oliveira, Guilherme G. C. Carvalho, Carlos R. K. Paier, Claudia Pessoa, Denise G. Guerreiro, Maria Elisabete A. Moraes, Jose Ricardo Figueiredo, Arlindo A. Moura
Summary: The study evaluated the proteome of early antral follicles from Ovis aries using LC-MS/MS analysis. A total of 2503 follicle proteins were identified, including abundant proteins such as vimentin, actin, lamin, heat shock proteins, and histones. miRNAs were found to modulate genes involved in cell cycle, cell differentiation, aging, apoptosis, adipocyte differentiation, and cell division in sheep follicles. This comprehensive atlas of proteins expressed in sheep early antral follicles will contribute to the identification of biomarkers for follicular development and oocyte maturation.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
L. V. S. Naupas, D. C. C. Brito, S. S. Souza, F. A. S. Brandao, R. F. Silva, C. C. L. Fernandes, G. M. Tetaping, B. G. Alves, J. Y. G. Silva, M. I. F. Guedes, D. Rondina, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study found that adding 100μM or 150μM ALA to the vitrification solution can protect sheep ovarian tissue, maintain follicle morphology, and promote follicular development after in vitro culture or autotransplantation. However, high levels of ALA may cause oxidative stress and tissue fibrosis after transplantation.
Article
Agriculture, Dairy & Animal Science
Luisa Miglio, Marco Aurelio Schiavo Novaes, Laritza Ferreira de Lima, Tatiane Moraes Arantes, Kele Amaral Alves, Francisca Elda Ferreira Dias, Marcelo Emilio Beletti, Jose Ricardo de Figueiredo
Summary: This study investigated the effects of zinc oxide nanoparticles functionalized with curcumin (ZnO(np) + CUR) on bovine in vitro embryo production and cellular antioxidant response during in vitro embryo culture (IVC). Cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in the presence of different concentrations of ZnO(np) + CUR. The results showed that the addition of 12μM ZnO(np) + CUR during IVC reduced intracellular ROS production and blastocyst production rate.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Cell Biology
A. N. P. Morais, L. F. Lima, A. F. B. Silva, L. L. Lienou, A. C. A. Ferreira, Y. F. Watanabe, D. C. Joaquim, B. G. Alves, A. F. Pereira, D. R. Alves, A. C. Oliveira, S. M. Morais, D. M. Magalhaes-Padilha, J. R. Figueiredo, E. L. Gastal
Summary: This study investigates the effects of carvacrol supplementation on in vitro maturation and embryo production in bovine oocytes. It was found that carvacrol supplementation increased the antioxidant capacity of the culture medium, but decreased the number of cells per blastocyst.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.