Article
Oceanography
Chunyan Zhao, Liang Chi, Yongshuang Xiao, Bing Li, Yunliang Lu, Yanting Cui, Wenqi Wang, Jun Li
Summary: This study investigated the morphology and histology of the brains in male and female turbot during different stages of gonadal development. The results showed distinct sex differences in the brain of turbot, particularly in terms of brain volume and pituitary size, which provide insights into the functioning of the hypothalamus-pituitary-gonadal axis.
ACTA OCEANOLOGICA SINICA
(2022)
Article
Agriculture, Dairy & Animal Science
Paolo Ronza, Jose Antonio alvarez-Dios, Diego Robledo, Ana Paula Losada, Roberto Romero, Roberto Bermudez, Belen G. Pardo, Paulino Martinez, Maria Isabel Quiroga
Summary: Blood gene expression analysis is an emerging tool for monitoring the health status of organisms, with potential applications in understanding immunity and diseases in fish. This study focused on analyzing the blood transcriptome of turbot, a commercially important flatfish, in healthy and diseased specimens parasitized by myxozoan. The results revealed important insights into turbot immunity and the ineffective response to the parasitic infection, highlighting the significance of blood transcriptomics in studying fish physiopathology.
Article
Fisheries
Patricia Pereiro, Raquel Lama, Antonio Figueras, Beatriz Novoa
Summary: IL-18 is a pro-inflammatory cytokine belonging to the IL-1 family, playing a significant role in regulating Th1-mediated immune responses. While its function is well established in mammals, its activity in teleost remains unclear, possibly due to the absence of this gene or its complex regulation in fish species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Chunyan Zhao, Sujie Zheng, Yongji Dang, Mengshu Wang, Yichao Ren
Summary: The findings of this study reveal the crucial role of igf3 in regulating gonadal development in turbot. In females, the expression of igfs significantly increased during oogenesis, while in males, igf3 maintained high expression during spermatogonia proliferation. These results provide a better understanding of the IGF system in teleost gonadal development.
Article
Fisheries
Jiayu Liu, Rui Shao, Yawen Lan, Xinmeng Liao, Jinjin Zhang, Kangsen Mai, Qinghui Ai, Min Wan
Summary: The study demonstrates that vitamin D-3 enhances immune responses in fish, leading to lower mortality and reduced bacterial load in infected fish. Additionally, VD3 supplementation promotes bactericidal activity and modulates immune cell responses, highlighting its potential as a defense mechanism against pathogen infection in fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Mingzhu Pan, Jiahuan Liu, Dong Huang, Yanlin Guo, Kai Luo, Mengxi Yang, Weihua Gao, Qiaoqing Xu, Wenbing Zhang, Kangsen Mai
Summary: This study characterized the foxo3 in turbot and investigated its functions in liver inflammation through both in vitro and in vivo experiments. The results suggest that FoxO3 may modulate LPS-activated hepatic inflammation in turbot by decreasing proinflammatory cytokines, levels of liver enzymes, and activating specific pathways, providing valuable insights for immune regulation and potential therapies for liver inflammation in fish.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yanshe Xie, Changhua Wu, Zicong Li, Zhenfang Wu, Linjun Hong
Summary: Sex determination is crucial for genetic transmission in mammals. Abnormal expression of sex-related genes can lead to disorders of sex development and infertility. However, recent research has focused on artificially regulating sex-related gene expression to predetermine the sex of animal offspring. This paper reviews the mechanisms underlying gonad and primordial germ cell development, aiming to provide new directions for treating disorders of sex development and infertility by manipulating the sex ratio of livestock offspring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Qiaomu Hu, Qing Xiao, Haifeng Tian, Dapeng Li, Zhong Li
Summary: The study identified the essential role of the dead end gene in Primordial germ cells (PGCs) migration and survival in Monopterus albus, providing a basis for the production of fast-growing and reproductively sterile M. albus.
Article
Fisheries
lhan Aydin, Rafet Cagri Oztuerk, Yahya Terzi, Ercan Kuecuek, Hamza Polat, Ilhan Altinok
Summary: This study investigated skeletal development and malformations in diploid and cold shock-induced triploid turbot during the early life stages. The results showed no difference in skeletal development between ploidy groups, with vertebral elements mostly remaining cartilaginous until 15 days post-hatch. Cold shock-induced triploid turbot had no disadvantage in terms of development and remained a feasible alternative for the turbot aquaculture industry. The most frequently encountered malformation was of the neural arch or spine, with significantly higher frequencies in diploid turbot in the caudal region.
Article
Fisheries
Ye Gao, Yaokuan Liu, Pengmei Wang, Zhaolan Mo, Jie Li, Shulan Liu, Guiyang Li, Ming Zhu
Summary: The research characterized streptococcosis caused by Streptococcus parauberis in farming fish in China, identified it as serotype III, and developed vaccines for turbot. The study provides important information for disease control and prevention of streptococcosis in aquaculture in China.
Article
Fisheries
Li'ang Yang, Lijuan Wang, Zhihao Wu, Zhiyu Hao, Zongcheng Song, Feng You, Runqing Yang
Summary: The study on genetic effects of inbreeding on growth trajectories in turbot revealed that inbreeding had a larger impact on body length compared to body weight and body width. The genetic effects were not linear with increasing inbreeding degrees and varied depending on the age of the fish. Additionally, there were low genetic correlations between growth traits in early growth days and later growth days.
Article
Fisheries
Junxia Yu, Xiaoli Liu, Ning Yang, Beibei Wang, Baofeng Su, Qiang Fu, Min Zhang, Fenghua Tan, Chao Li
Summary: TLR1 in turbot was characterized, showing wide expression in healthy tissues, strongest in spleen and head-kidney; strong responses to LPS in challenge experiments, varied responses to other pathogen-associated molecular patterns; subcellularly distributed in cytoplasm, membrane, and nucleus.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Yudong Jia, Jiawei Wang, Yuntao Gao, Bin Huang
Summary: Hypoxia stress showed sex dimorphism difference in turbot, affecting their hematological and biochemical responses, with some parameters returning to normal levels after 12 hours of reoxygenation.
Article
Fisheries
Lele Wu, Yunong Wang, Jun Li, Zongcheng Song, Shihong Xu, Changbin Song, Mingming Han, Haixia Zhao, Li Zhou, Yanfeng Wang, Xian Li, Xinlu Yue
Summary: This study investigated the influence of different LED spectra on the performance of juvenile turbot. It was found that blue spectrum resulted in the best growth performance, antioxidative activity, and pathogen resistance, while red and orange spectrum led to growth retardation and decreased antioxidative activity and pathogen resistance. Therefore, blue spectrum is recommended for rearing juvenile turbot in aquaculture systems to improve fish welfare.
Article
Biochemistry & Molecular Biology
Tapas Chakraborty, Sipra Mohapatra, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta
Summary: This study investigated the role of sxl in vertebrate gonadogenesis and sexuality using medaka, and identified two Sxl homologues, Sxl1a and Sxl1b. It was found that sxl1a specifically expressed in primordial germ cells (PGC) and played a role in germ cell numbers and sexual identity assignment through its association with estrogenic signaling and er beta 2. This study highlights the novel role of sxl1a in germ cell maintenance and sexual identity determination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chunyan Zhao, Shihong Xu, Yifan Liu, Yanfeng Wang, Qinghua Liu, Jun Li
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Chengcheng Feng, Shihong Xu, Yifan Liu, Yanfeng Wang, Wenqi Wang, Jingkun Yang, Chunyan Zhao, Qinghua Liu, Jun Li
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Environmental Sciences
Yifan Liu, Daoyuan Ma, Chunyan Zhao, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Yanfeng Wang, Qinghua Liu, Jun Li
MARINE ENVIRONMENTAL RESEARCH
(2017)
Article
Reproductive Biology
Chunyan Zhao, Qinghua Liu, Shihong Xu, Yongshuang Xiao, Wenqi Wang, Jingkun Yang, Yang Yang, Yanfeng Wang, Zongcheng Song, Jun Li
Article
Biochemistry & Molecular Biology
Liang Chi, Shihong Xu, Zhizhong Xiao, Fan Lin, Daoyuan Ma, Chunyan Zhao, Yongshuang Xiao, Qinghua Liu, Jun Li
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Yifan Liu, Daoyuan Ma, Chunyan Zhao, Wenqi Wang, Xuelei Zhang, Xiao Liu, Ying Liu, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Qinghua Liu, Jun Li
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2014)
Article
Environmental Sciences
Yifan Liu, Qinghua Liu, Shihong Xu, Yanfeng Wang, Chengcheng Feng, Chunyan Zhao, Zongcheng Song, Jun Li
Summary: The study investigated the submicroscopic structure of male germ cells and spermatogenesis in turbot, revealing interesting findings such as the early formation of sperm tail and the number of mitochondria in spermatozoa. The research also indicated a negative correlation between the vitality of mature sperm and the proportion of sperm deformity.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Oceanography
Chunyan Zhao, Shihong Xu, Yifan Liu, Chengcheng Feng, Yongshuang Xiao, Yanfeng Wang, Qinghua Liu, Jun Li
Summary: The study on turbot revealed the significant role of melatonin in regulating reproductive physiology by primarily targeting Mtnr receptors in the brain, gonad, and eye. Melatonin functions through the central nervous system in the early breeding season and peaks at the spawning stage. The findings provide a foundation for further exploration of fish melatonin receptors in gonadal development and gamete maturation.
ACTA OCEANOLOGICA SINICA
(2022)
Article
Fisheries
Guo Guangxin, Kexin Li, Qianhui Zhu, Chunyan Zhao, Chao Li, Zilong He, Songnian Hu, Yichao Ren
Summary: Plant extract oregano oil and probiotics can improve growth performance and disease resistance in cultured fish by modulating the gut microbiota and immune-related gene expression. The use of oregano oil or Bacillus coagulans as dietary additives shows potential health benefits for fish, enhancing growth and immunity through microbiota modulation.
Article
Agriculture, Multidisciplinary
Dongxue Xu, Ji Liu, Yuanxue Gu, Yanru Chen, Chunyan Zhao, Guohua Sun, Yichao Ren, Chao Li, Bin Xia
Summary: Juvenile sea cucumbers were fed different microalgae in a 130-day experiment, leading to varying growth rates and amino acid compositions. Analysis of carbon isotopes showed fluctuations in specific amino acids. The study sheds light on the metabolism routing and nutrient utilization in sea cucumbers during their juvenile stage.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Environmental
Ji Liu, Dongxue Xu, Yanru Chen, Chunyan Zhao, Lanhao Liu, Yuanxue Gu, Yichao Ren, Bin Xia
Summary: The study evaluated the adverse effects of dietary polystyrene nanoplastics and microplastics on sea cucumber growth, oxidative stress induction, immune response, ammonia detoxification, and bacterial pathogen resistance. It was found that PS-N/MPs significantly impacted the growth and development of sea cucumbers, with PS-NPs showing more toxic effects than PS-MPs. The findings provide valuable information for understanding the size-dependent toxic effects of PS-N/MPs and early risk warning on marine invertebrates.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Fisheries
Yichao Ren, Xianhui Men, Yu Yu, Bing Li, Yangen Zhou, Chunyan Zhao
Summary: The study investigated the effects of transportation on rainbow trout, finding that transportation induces stress responses and immune regulation in fish, manifested as changes in gene expression and alterations in oxidative damage and antioxidant capacity.
AQUACULTURE REPORTS
(2022)
Article
Endocrinology & Metabolism
Chunyan Zhao, Bin Wang, Yifan Liu, Chengcheng Feng, Shihong Xu, Wenqi Wang, Qinghua Liu, Jun Li
Summary: This study confirms the presence of two Kiss/Kissr systems in turbot, and suggests their positive involvement in controlling the reproduction of turbot. These findings provide preliminary information on how the Kiss/Kissr system regulates seasonal reproduction in turbot broodstock.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Fisheries
Qiang Fu, Yuqing Li, Shoucong Zhao, Haojie Wang, Chunyan Zhao, Pei Zhang, Min Cao, Ning Yang, Chao Li
Summary: In this study, the profiles of long non-coding RNA (lncRNA) in the intestine of turbot fish were investigated in response to Vibrio anguillarum infection. The results revealed differential expression of lncRNAs and mRNAs associated with immune-related genes, and enrichment of gene ontology terms and KEGG pathways related to immune responses. The findings provide a foundation for further exploration of lncRNA functions in teleost fish during bacterial infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Limnology
Zhao Chunyan, Xu Shihong, Feng Chengcheng, Liu Yifan, Yang Yang, Wang Yanfeng, Xiao Yongshuang, Song Zongcheng, Liu Qinghua, Li Jun
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2018)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
