Article
Agriculture, Dairy & Animal Science
K. K. Forrest, V. V. Flores, S. C. Gurule, S. Soto-Navarro, C. B. Shuster, C. A. Gifford, J. A. Hernandez Gifford
Summary: This study found that oocytes matured from high LPS follicles had a higher percentage of abnormal cells compared to those matured from low LPS follicles. Additionally, follicular E2 concentrations tended to be higher in high LPS follicles. However, mRNA transcript abundance for aromatase and beta-catenin was similar between the two groups.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Francesca Grasselli, Simona Bussolati, Stefano Grolli, Rosanna Di Lecce, Cecilia Dall'Aglio, Giuseppina Basini
Summary: Our study found that orexin B may play a role in modulating the redox status in granulosa and endothelial cells, and potentially be involved in follicular angiogenic events.
Article
Reproductive Biology
Michal Andrzej Kosior, Riccardo Esposito, Natascia Cocchia, Federica Piscopo, Valentina Longobardi, Nunzio Antonio Cacciola, Giorgio Antonio Presicce, Giuseppe Campanile, Hilde Aardema, Bianca Gasparrini
Summary: The aim of this study was to evaluate the seasonal effect on the metabolomic profile of the ovarian follicle in Italian Mediterranean buffalo and identify potential strategies to improve oocyte competence during the non-breeding season. Analysis of follicular fluid, follicular cells, cumulus cells, and oocytes revealed seasonal differences in metabolite content, suggesting alterations in metabolic pathways during the non-breeding season. Pathway enrichment analysis identified metabolites linked to glutathione, energy generation, amino acid metabolism, and phospholipid biosynthesis. The identification of positive and negative competence markers in the follicular fluid provides a basis for optimizing the follicular environment and improving oocyte competence during the non-breeding season.
Article
Agriculture, Dairy & Animal Science
Jasmine Fusi, Maria Cristina Veronesi, Alberto Prandi, Monica Probo, Massimo Faustini, Tanja Peric
Summary: This study investigated the changes in sexual hormones in dogs during puberty using hair and nail samples to analyze the concentrations of testosterone (T), 17 beta-estradiol (E2), and progesterone (P4). Traditional methods using blood samples are not suitable for long-term studies, but hair and nails have been identified as suitable retrospective biological specimens. The results showed significant increases in T levels in male dogs and E2 levels at puberty and P4 levels after puberty in female dogs. Hair and nail T and nail P4 concentrations could differentiate between male and female dogs at specific sampling times. This study demonstrates the usefulness of hair and nails as biological specimens for studying the changes in T, E2, and P4 during the peripubertal period in dogs.
Article
Public, Environmental & Occupational Health
Margot Guth, Tyler Pollock, Mandy Fisher, Tye E. Arbuckle, Maryse F. Bouchard
Summary: A study found a negative correlation between urinary paraben concentrations and serum hormone levels in girls, particularly estradiol, LH, and FSH. This suggests that parabens may impact the development and function of the endocrine system in females.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Agriculture, Dairy & Animal Science
Casey C. Read, J. Lannett Edwards, F. Neal Schrick, Justin D. Rhinehart, Rebecca R. Payton, Shawn R. Campagna, Hector F. Castro, Jessica L. Klabnik, Sarah E. Moorey
Summary: Incorporating a fixed-time artificial insemination protocol can improve reproductive management and genetics of the beef herd. However, some animals exposed to such protocols may not display estrus before insemination, resulting in reduced oocyte developmental competence and decreased pregnancy rates. This study investigated the effects of reduced follicle maturity on oocyte energy production and follicular fluid metabolic composition. The results showed that more mature follicles had increased ATP and metabolites involved in glucose metabolism, which may contribute to higher cleavage rates and embryo quality. Cattle induced to ovulate small, immature follicles had decreased oocyte developmental competence and lower embryo cleavage and quality. This study highlights the importance of follicle maturity on oocyte metabolic capacity and follicular fluid metabolome profiles.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Jing Xu, Mary B. Zelinski
Summary: In vitro follicle development (IVFD) is a valuable model for understanding folliculogenesis, screening ovarian toxicity, and preserving fertility and species. This review discusses the culture systems used in IVFD across various species and the improvements made in follicle growth and oocyte maturation through optimization of culture conditions. The evaluation of oocyte quality following IVFD, including epigenetic and genetic profile, maturation, embryonic development, and pregnancy outcomes, is summarized. The review also highlights the limitations and knowledge gaps in IVFD for proper oocyte development. This information contributes to the goal of producing competent oocytes with genetic integrity for successful embryonic development and healthy offspring.
BIOLOGY OF REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Bruce S. Shapiro, Melody A. Rasouli, Kajal Verma, Ankita Raman, Forest C. Garner, Martha Aguirre, Leah Kaye, Carrie Bedient
Summary: This study aimed to examine the relationship between the size of punctured ovarian follicles and embryology outcomes. The study found that punctures of follicles smaller than 12.5 mm rarely resulted in good-quality blastocysts, while punctures of follicles in the 19-24.5 mm range were associated with higher yields of good-quality blastocysts than the average. Other groups did not show significant differences from the average, and there was no correlation between follicle size and the ploidy of biopsied blastocysts.
FERTILITY AND STERILITY
(2022)
Article
Agriculture, Dairy & Animal Science
S. Venkata Ratna, T. Prasada Rao, Deepa Pathipati, T. V. Chaitanya Kumar, B. Rambabu Naik, A. V. N. Siva Kumar
Summary: The present study investigated the role of kisspeptin in promoting preantral follicle development in sheep. The results showed that kisspeptin supplementation, along with other growth factors, enhanced steroid production in cultured preantral follicles. The study also found that the expression of P450 aromatase gene and the synthesis of estradiol and progesterone varied at different stages of in vitro development.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Endocrinology & Metabolism
Qiyu Yang, Lixia Zhu, Meng Wang, Bo Huang, Zhou Li, Juan Hu, Qingsong Xi, Jing Liu, Lei Jin
Summary: This retrospective study observed the IVM process of 157 GV oocytes from 59 infertile couples and found that the duration of GV-MI of oocytes and the serum progesterone levels of patients significantly affected the developmental potential of the IVM oocytes. It is crucial to fertilize oocytes at the optimal maturation stage during in vitro maturation to improve the formation rate of high-quality embryos.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Sarah E. Moorey, Emma A. Hessock, J. Lannett Edwards
Summary: The physiological status of the preovulatory follicle has a major impact on oocyte competence, with changes in steroid hormone production, metabolome profiles, and proteome profiles likely supporting the oocyte's development and metabolic capability. This review discusses the relationship between bovine oocyte developmental competency and antral follicle progression, as well as the importance of the evolving preovulatory intrafollicular environment for fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Review
Obstetrics & Gynecology
Dulama Richani, Kylie R. Dunning, Jeremy G. Thompson, Robert B. Gilchrist
Summary: Metabolism is crucial for oocyte quality, with glucose being essential for COC maturation and energy production. Mitochondria are the main ATP source in oocytes, while cumulus cells play a vital role in maintaining ATP levels. New insights in oocyte lipid metabolism and innovative strategies in IVM have shown positive effects on oocyte competence.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Obstetrics & Gynecology
Chengjun Liu, Ketong Su, Lu Chen, Zijiao Zhao, Xin Wang, Chenfeng Yuan, Yuqi Liang, Huili Ji, Chunjin Li, Xu Zhou
Summary: The research identified 129 differentially expressed genes associated with high developmental competence of oocytes, and confirmed that COL1A2 and renin can be used to evaluate oocyte quality and embryo developmental competence through RNA sequencing and qRT-PCR.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Reproductive Biology
Anamaria-Cristina Herta, Nazli Akin, Katy Billooye, Laura Saucedo-Cuevas, Francesca Lolicato, Ingrid Segers, Ellen Anckaert, Johan Smitz
Summary: The study shows that oocytes from cultured mouse follicles can overcome complete denudation, restoring functional transzonal projections, with no significant differences in meiotic and developmental competence compared to those from intact cultured follicles. These findings provide good premises for developing successful strategies to rescue human oocyte competence in in vitro culture approaches.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Jiaxin Duan, Huali Chen, Dejun Xu, Yuan Li, Xiaoya Li, Jianyong Cheng, Rongmao Hua, Zelin Zhang, Li Yang, Qingwang Li
Summary: The study found that 17β-estradiol can promote the development and maturation of porcine oocytes, enhance autophagy, and reduce oxidative stress levels and apoptosis activity in vitro.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.