Journal
THERIOGENOLOGY
Volume 80, Issue 4, Pages 302-312Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2013.04.012
Keywords
Two-step culture system; Conventional IVM system; PMC; Embryonic development; Gap junction communication
Categories
Funding
- Royan Institute of IRI
Ask authors/readers for more resources
The present study aims to investigate if prematuration culture (PMC) of ovine oocytes in the presence of a phosphodiesterase type 3 (PDE3) inhibitor cilostamide can improve the shortcomings of conventional in vitro maturation (IVM) system. Therefore, a two-step culture system consisting of 22 hours culture in the presence of 1, 10, and 20 mu M cilostamide (PMC medium), followed by 22 hours culture in maturation medium, was designed. The effect of cilostamide on gap junction communications and nuclear status was studied. The variables assessed were chromosome organization, spindle pattern, polar body extrusion, and embryonic development. According to the results, inhibition of PDE3 could not permanently block nuclear maturation in ovine oocytes but it delayed the process of nuclear maturation. Elevation of intra-oocyte cAMP concentration could inhibit cumulus cells expansion and maintain gap junction communications between oocyte and cumulus cells. Deletion of cilostamide and refreshing maturation medium after 22 hours culture revealed that cumulus cells were completely expanded. The inhibitory effect induced by 1 mu M cilostamide was reversible, and it increased the number of Mature oocytes with aligned chromosomes and normal spindle. However, the inhibitory effects of 10 and 20 mu M cilostamide was not fully reversible and was associated with deleterious effects on chromosome organization and spindle pattern. Investigation of embryonic development via parthenogenetic activation and in vitro fertilization revealed that the blastocyst rate of oocytes that were prematured with 1 mu M cilostamide was not significantly different from oocytes that underwent converitional IVM but it was significantly reduced in oocytes that. were prematured with 10 and 20 mu M cilostamide. Our results provide the evidence that reduced cAMP via PDE3 is not the only mechanism that controls the progress of nuclear maturation in sheep oocytes, and that alternative or additional mechanisms may also exist. (C) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available