Article
Reproductive Biology
T. Castro, J. C. Jacob, G. Stefani, R. R. Domingues, O. J. Ginther
Summary: The concentrations of progesterone (P4) and a metabolite of PGF2 alpha (PGFM) in mares show significant variations between the interovulatory interval (IOI) and early pregnancy. The study reveals different patterns of PGFM concentration fluctuations between the IOI and pregnancy, with pregnant mares exhibiting smaller PGFM pulse amplitudes.
Article
Agriculture, Dairy & Animal Science
Maria Rosa Rebordao, Ana Amaral, Carina Fernandes, Elisabete Silva, Karolina Lukasik, Anna Szostek-Mioduchowska, Pedro Pinto-Bravo, Antonio Galvao, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: PGF(2 alpha) and PTGFR expression in mare endometrium are influenced by endometrial category and estrous cycle phase. NETs enzymes may modulate PGF(2 alpha) pathway activation and fibrogenesis in the endometrium.
Article
Chemistry, Multidisciplinary
Ana Claudia Lima, Diana Amorim, Ines Laranjeira, Armando Almeida, Rui L. Reis, Helena Ferreira, Filipa Pinto-Ribeiro, Nuno M. Neves
Summary: The immobilization of anti-TNF-α and anti-IL-6 antibodies on polymeric nanoparticles extended and increased therapeutic efficacy while avoiding off-target effects. Biofunctionalized nanoparticles showed greater efficiency in reducing inflammation compared to soluble antibodies, offering a promising therapeutic option for inflammatory conditions with a safe profile and prolonged action.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Immunology
Takashi Ashino, Yuki Nakamura, Hirokazu Ohtaki, Yoichiro Iwakura, Satoshi Numazawa
Summary: Sepsis affects drug metabolism and the expression of cytochrome P450s. The study shows that cecal ligation and puncture (CLP) induces the expression of proinflammatory cytokines and leads to decreased survival in IL-6(-/-) mice. CLP also downregulates the expression of Cyp2c and Cyp3a11 genes, but this effect is abolished by IL-6 depletion.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Immunology
Wanvisa Udomsinprasert, Jiraphun Jittikoon, Sermsiri Sangroongruangsri, Usa Chaikledkaew
Summary: The levels of IL-6 and IL-10 in COVID-19 patients may serve as potential biomarkers for disease severity and mortality.
JOURNAL OF CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Selma Schmidt, Heinrich Kreutzmann, Maria Stadler, Kerstin H. Mair, Melissa R. Stas, Michaela Koch, Eleni Vatzia, Sophie Duerlinger, Christian Knecht, Joachim Spergser, Marlies Dolezal, Sven Springer, Tobias Theuss, Vicky Fachinger, Andrea Ladinig, Armin Saalmueler, Wilhelm Gerner
Summary: The study demonstrates that the Salmoporc vaccine can induce a strong CD4(+) T-cell response, contributing to local immunity and limiting the spread of STM to lymph nodes and systemic organs. Significant differences in cytokine-producing T-cell phenotypes were observed when comparing vaccinated and infected animals with infected-only animals.
Article
Cardiac & Cardiovascular Systems
Agnieszka Jablonska, Branislav Zagrapan, Christoph Neumayer, Wolf Eilenberg, Andreas Scheuba, Christine Brostjan, Svitlana Demyanets, Markus Klinger, Josif Nanobachvili, Ihor Huk
Summary: The study found that mutations in IL-6 -174G/C genotype were associated with an increased risk of AAA occurrence, while heterozygous carriers of TNF-α -308G/A genotype also showed an increased risk of AAA incidence. The wild-type genotypes of IL-6 -174G/C and TNF-α -308G/A appeared more frequently in healthy subjects, and were associated with a decreased risk of AAA.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Education, Scientific Disciplines
Takashi Ishijima, Kazuyuki Nakajima
Summary: Through utilizing an animal model with LPS-induced rat brain, the study revealed that microglia induce different inflammatory cytokines through specific combinations of MAPKs and by the presence or absence of nitric oxide.
Article
Dentistry, Oral Surgery & Medicine
Chenlu Shen, Weijia Ye, Lijun Gong, Kejia Lv, Bicong Gao, Hua Yao
Summary: The study revealed significantly elevated levels of serum interleukin-6 and tumor necrosis factor-alpha in patients with recurrent aphthous stomatitis, with no significant difference in interleukin-17A levels between the two groups. Further exploration is needed to understand the role of TNF-alpha in the pathology of RAS.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2021)
Article
Plant Sciences
Ying Feng, Han Dong, Liyan Zheng
Summary: Ligustrazine was found to inhibit inflammation in Endometriosis (EMs) by regulating the STAT3/IGF2BP1/RELA axis. These findings propose a new agent against EMs and support the development of ligustrazine-based treatment strategies for EMs.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Anesthesiology
Siqi Xu, Shenghong Hu, Xia Ju, Yuanhai Li, Qing Li, Shengbin Wang
Summary: The combination of lidocaine and dexmedetomidine significantly alleviated inflammatory responses, decreased postoperative pain, and led to fewer postoperative nausea and vomiting in patients undergoing laparoscopic hysterectomy.
BMC ANESTHESIOLOGY
(2021)
Article
Medicine, General & Internal
Weronika Pawlik, Jakub Pawlik, Mateusz Kozlowski, Karolina Luczkowska, Sebastian Kwiatkowski, Ewa Kwiatkowska, Boguslaw Machalinski, Aneta Cymbaluk-Ploska
Summary: This study found that serum levels of IL-8 and TNF-alpha were significantly higher in ovarian cancer patients compared to patients with benign ovarian cysts, and they can be used as new useful markers for predicting ovarian cancer. IL-8 and TNF-alpha have a sensitivity and specificity of 70% and 96%, and 85.7% and 79.3% respectively, indicating their utility in predicting the malignant nature of ovarian lesions.
Article
Immunology
Carol G. Chitko-McKown, Stacy L. Bierman, Larry A. Kuehn, Gary L. Bennett, Keith D. DeDonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers
Summary: The study aimed to develop a multiplex assay for bovine inflammatory cytokines IL-113, IL-6, and TNF alpha using the U-PLEX platform. Commercially available Do-It-Yourself ELISA kits containing polyclonal antibodies and specific recombinant bovine cytokine standards were purchased for each cytokine. The U-PLEX platform proved to be a viable means for developing custom analyte- and species-specific multiplex assays.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Gastroenterology & Hepatology
Simon Johannes Gairing, Julian Anders, Leonard Kaps, Michael Nagel, Maurice Michel, Wolfgang Maximilian Kremer, Max Hilscher, Peter Robert Galle, Joern M. Schattenberg, Marcus-Alexander Woerns, Christian Labenz
Summary: Using IL-6 serum levels as a biomarker in a stepwise diagnostic algorithm can reduce the number of patients requiring testing with PHES and in turn the workload.
HEPATOLOGY COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ayami Saga, Masahiro Koseki, Kotaro Kanno, Jiuyang Chang, Tomoaki Higo, Daisuke Okuzaki, Takeshi Okada, Hiroyasu Inui, Masumi Asaji, Katsunao Tanaka, Takashi Omatsu, Sae Nishihara, Yinghong Zhu, Kaori Ito, Hiroaki Hattori, Ikuyo Ichi, Yoshihiro Kamada, Masafumi Ono, Toshiji Saibara, Tohru Ohama, Shungo Hikoso, Makoto Nishida, Shizuya Yamashita, Yasushi Sakata
Summary: Patients with psoriasis are more prone to nonalcoholic fatty liver disease. A cholesterol derivative called 7-ketocholesterol (7KC) may exacerbate psoriasis-like dermatitis by accelerating steatohepatitis. These findings suggest a diet-related factor linking steatohepatitis and psoriasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Agata Zmijewska, Wioleta Czelejewska, Mariusz Dziekonski, Alina Gajewska, Anita Franczak, Stanislaw Okrasa
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Anita Franczak, Ewa M. Waszkiewicz, Wiktoria Kozlowska, Agata Zmijewska, Anna Koziorowska
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anna Koziorowska, Anita Franczak
Summary: This study investigated the effects of EMF on androgen synthesis and release from pig endometrium, finding that EMF may have suppressive effects on endometrial testosterone release and increased androstenedione release. Progesterone did not protect the pig endometrium against EMF radiation during the fetal peri-implantation period.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Reproductive Biology
Justyna Kolakowska, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Serhiy Souchelnytskyi, Anita Franczak
Summary: The study aimed to find unique proteins in porcine conceptuses specifically during the peri-implantation period, leading to the discovery of 24 unique proteins involved in processes like adhesion, cadherin, and actin filament organization. These unique proteins may play a role in embryo development and acquisition of implantation competence.
REPRODUCTIVE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
Summary: Electromagnetic fields can alter the expression levels of CYP19A3 and HSD17B4 in pig myometrium during the peri-implantation period, while progesterone may mitigate the effects of EMF, regulating the expression levels of aromatase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: KISS and RFRP-3 systems are present in the pituitary of estrous-cyclic and pregnant pigs, potentially affecting the synthesis and secretion of FSH by pituitary cells.
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak
Summary: The study found that EMF treatment affects gene expression in the myometrium of pigs during the peri-implantation period, mainly involving defense and immune responses. Analysis revealed that DEGs enrich multiple signaling pathways, and also uncovered the regulatory mechanisms of EMF through long non-coding RNAs and single nucleotide variants on the transcriptomic profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
Summary: This study revealed that dietary restriction during the peri-conceptional period causes changes in gene expression in the pig myometrium, particularly those involved in endocrine activity, embryo-maternal interactions, and the regulation of cell cycle and proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Reproductive Biology
Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anita Franczak
Summary: The study aims to investigate the effects of EMF exposure on estrogen synthesis and release in the endometrium. The results suggest that EMF exposure affects gene expression and is related to P-4 treatment. Additionally, EMF disrupts the synthesis and release of E-1 and E-2 in the endometrium.
REPRODUCTIVE BIOLOGY
(2022)
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: The pituitary gland plays a crucial role in female reproduction. However, little is known about the changes in the transcriptome of the porcine pituitary during reproductive status. This study aimed to analyze and compare the transcriptome profile of the pituitary gland in pregnant pigs and pigs during the estrous cycle. The analysis revealed 482 differentially expressed genes, with 68 up-regulated and 414 down-regulated in pregnant pigs. These findings provide insights into the molecular mechanisms involved in maternal recognition of pregnancy in pigs.
Article
Reproductive Biology
Anita Franczak, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski
Summary: Previous research has shown that the ELF-EMF can induce molecular alterations in the porcine myometrium. In this study, it was found that exposure to ELF-EMF increased DNMT1 expression, decreased DNMT3a expression, and increased genomic DNA methylation level in porcine myometrium. Moreover, the amplification level of methylated and unmethylated variants of the promoter region of selected genes was also affected by ELF-EMF radiation.
Article
Developmental Biology
Pawel Jozef Wydorski, Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anita Franczak
Summary: Short-term ELF-EMF treatment may alter the DNA methylation level of genes in the endometrium. The changes in DNA methylation induced by ELF-EMF may affect the transcriptomic profile of the endometrium and disrupt physiological processes such as implantation and embryo development.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2023)
Article
Physiology
M. Martyniak, E. M. Drzewiecka, A. Zmijewska, A. Franczak, G. Kotwica
Summary: This study found that the gene expression in the epithelial cells of the ampulla and isthmus of the oviducts in pigs was altered during the peri-conceptional period, possibly due to DNA methylation. By evaluating important genes related to embryo development and determining the level of DNA methylation, it was found that the methylation levels of certain genes have changed. This suggests that the presence of embryos in the oviduct can change the transcriptomic profile and DNA methylation level in the epithelial cells.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Developmental Biology
Anita Franczak, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anna Koziorowska
Summary: This study demonstrates that electromagnetic field radiation affects the steroidogenesis and release from pig conceptuses during the pre-implantation period. It also suggests that progesterone may have a protective effect against the effects of electromagnetic field radiation.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Physiology
W. Kozlowska, E. M. Drzewiecka, L. Paukszto, A. Zmijewska, P. J. Wydorski, J. P. Jastrzebski, A. Franczak
Summary: This study found that exposure to low-frequency electromagnetic field alters the gene expression profile of pig endometrium, specifically affecting genes encoding proteins involved in proliferation and metabolism.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.