Article
Biochemistry & Molecular Biology
Dhani Ram Mahato, Magnus Andersson
Summary: The function of Na+,K+-ATPases in biological membranes depends on the surrounding lipid environment. Lipid-interaction sites A and B have been confirmed to activate and stabilize the protein, while the location of inhibitory site C has yet to be experimentally determined. Disease mutants in the membrane-protein interface can also affect lipid interactions. In this study, simulations were used to determine the lipid-protein interactions of wild-type and disease mutant Na+,K+-ATPases, revealing that lipid interactions are highly state-dependent and can be disrupted by disease mutations in the lipid interface.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Cell Biology
September Numata, Jeffrey P. McDermott, Gustavo Blanco
Summary: The NKA alpha 4 isoform is specifically expressed in male germ cells and plays a crucial role in sperm motility and energetics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Clinical Neurology
Samira S. Valvassori, Jefte Peper-Nascimento, Jorge M. Aguiar-Geraldo, Amanda Hilsendeger, Thiani Daminelli, Mario F. Juruena, Rif S. El-Mallakh, Joao Quevedo
Summary: This study found significant differences in biological rhythms, Na+, K+-ATPase, and oxidative stress parameters between bipolar disorder patients and healthy controls, highlighting the importance of these parameters in relation to BD.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Inga Baasch Christensen, Lei Cheng, Jonathan R. Brewer, Udo Bartsch, Robert A. Fenton, Helle H. Damkier, Jeppe Praetorius
Summary: The research revealed that choroid plexus epithelium expresses multiple Na,K-ATPase subunits, forming complexes that interact with the microvillar cytoskeleton through actin or other proteins. Additionally, the expression of NKCC1 is necessary for the accumulation of Na,K-ATPase in the choroid plexus brush border.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Priya Gupta, Archisman Mahapatra, Anjali Suman, Rahul Kumar Singh
Summary: This study investigated the effects of cypermethrin, a synthetic pesticide, on the development of zebrafish embryos. The results showed that cypermethrin exposure led to abnormal development, decreased heart rate, and disruption of cardiac development and ion regulation. Computational docking also revealed interactions between cypermethrin and developmental proteins. This study provides important information on the ecotoxicological risk and the mode of action of cypermethrin.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Aline Yammine, Lizette Auezova, Gerard Lizard, Helene Greige-Gerges
Summary: The aim of this study was to investigate whether quercetin, resveratrol, or caffeic acid, at nano-and low micromolar concentrations, can improve the activity of Na+/K+- and Ca2+-ATPases in human erythrocyte membranes with excess cholesterol. These polyphenols belong to different chemical classes and are widely present in plant foods.
Article
Biochemistry & Molecular Biology
Sofia Ygberg, Evgeny E. Akkuratov, Rebecca J. Howard, Fulya Taylan, Daniel C. Jans, Dhani R. Mahato, Adriana Katz, Paula F. Kinoshita, Benjamin Portal, Inger Nennesmo, Maria Lindskog, Steven J. D. Karlish, Magnus Andersson, Anna Lindstrand, Hjalmar Brismar, Anita Aperia
Summary: The Trp931Arg mutation of the Na+,K+-ATPase catalytic alpha 1 subunit described in this study led to reduced membrane expression of the mutant protein, loss of ion pumping activity, and formation of water-filled pores in the plasma membrane. This resulted in depolarization of neurons, a lowered threshold for epileptic seizures, and destabilization of the ion-binding sites. These findings suggest that Na+,K+-ATPase plays a significant role in diseases associated with epilepsy and loss of plasma membrane integrity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Obstetrics & Gynecology
T. Hirakawa, M. Goto, K. Takahashi, T. Iwasawa, A. Fujishima, K. Makino, H. Shirasawa, W. Sato, T. Sato, Y. Kumazawa, Y. Terada
Summary: The study investigated the expression and localization of specific Na+/K+ ATPase isoforms in human blastocysts and their association with embryonic developmental behavior. The results showed that Na+/K+ ATPase alpha 1, beta 1, and beta 3 isoforms were expressed in human blastocysts, but their expression levels were not correlated with blastocyst expansion rate. The alpha 1 and beta 3 isoforms were localized to the basolateral membrane of trophectoderm cells, while the beta 3 isoform was localized between trophectoderm cells.
HUMAN REPRODUCTION
(2022)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Elias Rizk, Andy Madrid, Joyce Koueik, Dandan Sun, Krista Stewart, David Chen, Susan Luo, Felissa Hong, Ligia A. Papale, Nithya Hariharan, Reid S. Alisch, Bermans J. Iskandar
Summary: FACS-based sorting was used to investigate the regulation of axon regeneration in retinal ganglion cells (RGCs) following optic nerve transection in rats. The study revealed the involvement of Na+/K+-ATPase and DNA methylation in axon regeneration, with specific genes and pathways identified. Inhibition of Na+/K+-ATPase led to reduced RGC axon regeneration, highlighting its importance as a key regulator of CNS axon regeneration. Reactivation of embryonic signals was also found to be necessary for successful regeneration. These findings provide critical insights into the molecular mechanisms of axon regeneration in the mammalian CNS.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Violetta V. Kravtsova, Arina A. Fedorova, Maria Tishkova, Alexandra A. Livanova, Oleg V. Vetrovoy, Alexander G. Markov, Vladimir V. Matchkov, Igor I. Krivoi
Summary: This study investigated the protective effect of ouabain on ionizing radiation-induced disturbances in skeletal muscle Na,K-ATPase function. Ouabain was found to prevent depolarization and loss of electrogenic contribution of the alpha 2 isozyme of Na,K-ATPase, accompanied by an increase in circulating ouabain concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.