Article
Biochemistry & Molecular Biology
Jaime Catalan, Ivan Yanez-Ortiz, Asta Tvarijonaviciute, Luis Guillermo Gonzalez-Arostegui, Camila P. Rubio, Isabel Barranco, Marc Yeste, Jordi Miro
Summary: This study examined the relationship between enzymatic and non-enzymatic antioxidants in seminal plasma and horse sperm's ability to withstand freeze-thawing. The results showed that enzymatic antioxidants PON1 and SOD, as well as non-enzymatic antioxidant TEAC, were positively correlated with sperm motility and plasma membrane integrity. On the other hand, the oxidative stress index (OSI) was negatively correlated with these parameters and positively correlated with plasma membrane lipid disorder. Further investigation is needed to explore the potential use of these antioxidants and OSI in predicting sperm cryotolerance in horses.
Article
Fisheries
Bruna Bitencourt Da Costa, Lis Santos Marques, Paula Graziela Lassen, Romulo Batista Rodrigues, Helen Tais Da Rosa-Silva, Jose Claudio Fonseca Moreira, Diogo Losch de Oliveira, Danilo Pedro Streit
Summary: The study aimed to investigate the effects of different concentrations of glutamine (experiment 1) and glutamine + cysteine (experiment 2) on post-thaw sperm quality of South American silver catfish. Results showed that glutamine supplementation had mixed effects on sperm quality, with increased DNA fragmentation but also higher enzyme activity. Cysteine and glutamine together did not show satisfactory results and had damaging effects on sperm quality.
AQUACULTURE RESEARCH
(2021)
Article
Reproductive Biology
Saurabh Tiwari, R. K. Dewry, Rashika Srivastava, Sapna Nath, T. K. Mohanty
Summary: Mitochondria play crucial roles in sperm fertilizing competence, sperm quality, and semen cryopreservation. Targeted antioxidants for mitochondria can effectively alleviate oxidative stress and improve semen freezing efficiency.
Article
Biochemistry & Molecular Biology
Monika Bartekova, Adriana Adameova, Aniko Gorbe, Kristina Ferenczyova, Olga Pechanova, Antigone Lazou, Naranjan S. Dhalla, Peter Ferdinandy, Zoltan Giricz
Summary: This review discusses the impact of CMDs on cardiac redox homeostasis, the role of OxS in cardiac pathologies, and the potential therapeutic effects of natural and synthetic antioxidants in CMDs. Despite numerous preclinical and clinical studies, no antioxidant therapy for the prevention and treatment of CMDs has been successfully translated, highlighting a significant translational gap in this field.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Environmental Sciences
Rohit Gautam, Eepsita Priyadarshini, Jay Prakash Nirala, Ramovatar Meena, Paulraj Rajamani
Summary: The study found that continuous exposure to electromagnetic radiation can lead to oxidative stress, inducing biochemical changes in rat sperms. However, Punica granatum extract has a protective role against oxidative damage induced by EM radiation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Angela Allen, Shiromi Perera, Sachith Mettananda, Rexan Rodrigo, Lakshman Perera, Thamal Darshana, Fiona Moggach, Anthony Jackson Crawford, Lesley Heirene, Christopher Fisher, Nancy Olivieri, David Rees, Anuja Premawardhena, Stephen Allen
Summary: In patients with beta-thalassemia in Sri Lanka, oxidative stress and damage are common, leading to complications such as anemia, hypoxia, and inflammation. Most patients lack adequate intake of fresh fruits and vegetables, and overall physical fitness is poor.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Veterinary Sciences
Yentel Mateo-Otero, Marc Yeste, Anna Damato, Elisa Giaretta
Summary: This review discusses the impact of cryopreservation on the redox balance of pig oocytes, focusing on the relevance of non-enzymatic and enzymatic antioxidant defenses. It also considers the regulation of gene expression in response to oxidative stress. Finally, the effects of supplementing maturation and vitrification media with exogenous non-enzymatic antioxidants are discussed.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Chaojun Yang, Xiaocan Jia, Yuping Wang, Jingwen Fan, Chenyu Zhao, Yongli Yang, Xuezhong Shi
Summary: This study found a relationship between deficiency of dietary total antioxidant capacity (TAC) and increased risk of stroke in US adults, particularly in subgroups of individuals aged over 60, females, alcohol consumers, former smokers, and inactive individuals.
Review
Cell Biology
Beijia Cao, Jianpeng Qin, Bo Pan, Izhar Hyder Qazi, Jiangfeng Ye, Yi Fang, Guangbin Zhou
Summary: Oocyte cryopreservation induces oxidative stress and reduces the developmental potential of oocytes. While oocytes have intrinsic antioxidant systems, cryopreservation still causes oxidative damage. Exogenous antioxidants, such as melatonin and resveratrol, can reduce oxidative damage. This review discusses the analysis of oxidative stress induced by oocyte cryopreservation, the impact of antioxidants against oxidative damage, and their underlying mechanisms, providing a reference for improving the efficiency of oocyte cryopreservation.
Review
Biochemistry & Molecular Biology
Roberto Gualtieri, Guruprasad Kalthur, Vincenza Barbato, Maddalena Di Nardo, Satish Kumar Adiga, Riccardo Talevi
Summary: Mitochondria play a crucial role in cell metabolism and ATP synthesis, while also being responsible for generating ROS and maintaining calcium homeostasis. Dysfunction in mitochondria can lead to oxidative stress, affecting cell function. Assisted reproductive technologies and cryopreservation may compromise mitochondrial function, impacting reproductive outcomes by affecting sperm and oocyte quality and fertilization dynamics.
Review
Biochemistry & Molecular Biology
Alexander J. J. Didier, Jennifer Stiene, Lauren Fang, Dean Watkins, Lance D. D. Dworkin, Justin F. F. Creeden
Summary: Oxidative stress, characterized by an imbalance between pro-oxidant molecules and antioxidant defense systems, is considered a major contributor to cancer development. Antioxidant supplementation, such as vitamins A, C, and E, has shown potential in reducing the risk of certain types of cancer, although there are conflicting findings. This review provides an overview of the role of oxidative stress in cancer formation and discusses the potential benefits and limitations of preventive antioxidant strategies.
Review
Biochemistry & Molecular Biology
Muhammad Zahoor Khan, Yulin Ma, Jianxin Xiao, Tianyu Chen, Jiaying Ma, Shuai Liu, Yajing Wang, Adnan Khan, Gibson Maswayi Alugongo, Zhijun Cao
Summary: The article reviews the causes of mastitis in periparturient dairy cattle and discusses the role of oxidative stress. Supplementing dairy cattle with antioxidants during the periparturient period has been found to effectively alleviate oxidative stress and mastitis.
Article
Sport Sciences
Aleksandra Filip-Stachnik, Michal Krzysztofik, Juan Del Coso, Tomasz Palka, Ewa Sadowska-Krepa
Summary: This study examined the impact of caffeine intake on prooxidant-antioxidant balance and muscle damage following resistance exercise. The results showed that consuming 3 mg/kg of caffeine did not significantly affect the number of repetitions, prooxidant-antioxidant balance, or muscle damage markers.
JOURNAL OF SPORTS SCIENCE AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Marta Budkowska, Elzbieta Cecerska-Heryc, Zuzanna Marcinowska, Aldona Siennicka, Barbara Dolegowska
Summary: This study aimed to investigate the activity of several enzymes related to oxidative stress and the concentration of glutathione in the circadian rhythm. The results showed that the activity of these enzymes and the concentration of glutathione peaked at 2 a.m. during sleep. This suggests that the processes maintaining the redox balance are intensified during sleep, thus reducing oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Manal El-Zohri, Naseem A. Al-Wadaani, Sameera O. Bafeel
Summary: This study found that foliar spray with green zinc oxide nanoparticles enhanced tomato tolerance to drought stress, particularly at concentrations of 25 and 50 mg/L of ZnO-NPs. These concentrations proved to be the most effective in promoting plant growth and alleviating oxidative stress.
Article
Developmental Biology
Patricia Diogo, Gil Martins, Ana Eufrasio, Tome Silva, Elsa Cabrita, Paulo Gavaia
Article
Developmental Biology
Gil Martins, Patricia Diogo, Wilson Pinto, Paulo J. Gavaia
Article
Environmental Sciences
Marta Lombo, Silvia Gonzalez-Rojo, Cristina Fernandez-Diez, Maria Paz Herraez
ENVIRONMENTAL POLLUTION
(2019)
Article
Environmental Sciences
S. Gonzalez-Rojo, M. Lombo, C. Fernandez-Diez, M. P. Herraez
ENVIRONMENTAL POLLUTION
(2019)
Article
Biochemistry & Molecular Biology
Marta Lombo, Lidia Getino-Alvarez, Alexandra Depince, Catherine Labbe, Maria Paz Herraez
Article
Biology
Patricia Diogo, Gil Martins, Rita Nogueira, Ana Marreiros, Paulo J. Gavaia, Elsa Cabrita
Article
Developmental Biology
Gil Martins, Patricia Diogo, Tamara Santos, Elsa Cabrita, Wilson Pinto, Jorge Dias, Paulo J. Gavaia
Article
Multidisciplinary Sciences
Marta Lombo, Cristina Fernandez-Diez, Silvia Gonzalez-Rojo, Maria Paz Herraez
SCIENTIFIC REPORTS
(2019)
Article
Biology
Marta Lombo, Paz Herraez
Summary: Environmental pollution, specifically endocrine-disrupting chemicals, poses a threat to male reproductive health by affecting sperm motility, sexual function, and gonad development. The consequences of paternal exposure to these harmful chemicals for future generations remain largely unknown. Sperm, traditionally viewed as simple carriers of genetic material, are now recognized to play a crucial role in offspring development and long-term health, making them a key target for EDCs and their potential impact on subsequent generations.
BIOLOGICAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Marta Lombo, Maria Paz Herraez
Summary: Parental exposure to BPA has been linked to a greater incidence of congenital diseases. In zebrafish males, BPA induces an increase in sperm histone acetylation that is transmitted to unexposed progeny. Histone hyperacetylation promoted by paternal BPA exposure alters chromatin structure in progeny, leading to overexpression of genes involved in cardiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Public, Environmental & Occupational Health
Joana Margarida Boto, Ana Marreiros, Patricia Diogo, Ezequiel Pinto, Maria Palma Mateus
Summary: The study identified that daily intake of two portions of fruits and vegetables is a determinant health behavior for high adherence to the Mediterranean diet among adolescents. Strategies to promote the intake of these foods among adolescents are necessary.
PUBLIC HEALTH NUTRITION
(2022)
Article
Environmental Sciences
Enrique Escarda-Castro, Maria Paz Herraez, Marta Lombo
Summary: This study investigated the genotoxic and epigenotoxic effects of bisphenol A (BPA) during cardiomyocyte differentiation. The results showed that BPA exposure led to changes in gene expression related to heart differentiation, as well as DNA damage and alteration in histone acetylation. BPA seriously interferes with the process of cardiomyocyte differentiation, potentially impacting cardiogenesis in vivo.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biology
Catarina Anjos, Daniel Duarte, Patricia Diogo, Domitilia Matias, Elsa Cabrita
Summary: This study successfully established cryopreservation protocols for D-larvae of C. angulata and C. gallina. The results showed that ethylene glycol was the best cryoprotectant for C. angulata, while dimethyl sulfoxide produced better results for C. gallina.
Article
Marine & Freshwater Biology
Laura Ribeiro, Francois Noel Hubert, Vera Rodrigues, Carlos Rojas-Garcia, Maria Teresa Dinis
Summary: This study analyzed the effects of time, light, and water temperature on the feeding incidence and intensity of gilthead seabream larvae, as well as the content of cholecystokinin (CCK). The results showed that light is important for the feeding ability of gilthead seabream larvae until a certain age, and the functionality of CCK matures at later stages.
OCEANS-SWITZERLAND
(2022)
Article
Andrology
Silvia Gonzalez-Rojo, Cristina Fernandez-Diez, Marta Lombo, Maria Paz Herraez
ASIAN JOURNAL OF ANDROLOGY
(2020)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.