Article
Agriculture, Dairy & Animal Science
Alejandro Gonzalez-Plaza, Cristiano Brullo, Josep M. Cambra, Manuela Garcia, Eleonora Iacono, Inmaculada Parrilla, Maria Antonia Gil, Emilio A. Martinez, Cristina A. Martinez, Cristina Cuello
Summary: This study aimed to investigate the effects of different equilibration times with cryoprotectants on viability and metaphase plate morphology of vitrified-warmed porcine mature oocytes, and to evaluate the effects of melatonin supplementation during in vitro maturation on these parameters. The results showed that vitrified porcine mature oocytes equilibrated for 60 or 80 min exhibited the highest viability and similar metaphase plate characteristics to fresh controls. Furthermore, supplementation with melatonin during in vitro maturation had no effect on these parameters.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Biochemistry & Molecular Biology
Lucia Olexikova, Linda Dujickova, Alexander V. Makarevich, Jiri Bezdicek, Jana Sekaninova, Andrea Nesvadbova, Peter Chrenek
Summary: This study aimed to investigate whether adding the antioxidant glutathione to devitrified oocytes can improve further embryo development. The results showed that adding 5 mmol L-1 of glutathione significantly increased the total antioxidant capacity of warmed oocytes and improved blastocyst development. These findings suggest that treating devitrified oocytes with antioxidants can enhance their development.
Article
Agriculture, Dairy & Animal Science
Min-Jee Park, Seung-Eun Lee, Jae-Wook Yoon, Hyo-Jin Park, So-Hee Kim, Seung-Hwan Oh, Do-Geon Lee, Da-Bin Pyeon, Eun-Young Kim, Se-Pill Park
Summary: This study found that the addition of HPC to the vitrification solution can improve the survival rate of VT bovine oocytes and the development capacity of embryos. This has significant implications for successful cryopreservation in research and commercial applications.
Article
Biochemistry & Molecular Biology
Xi Xu, Baigao Yang, Hang Zhang, Xiaoyi Feng, Haisheng Hao, Weihua Du, Huabin Zhu, Adnan Khan, Muhammad Zahoor Khan, Peipei Zhang, Xueming Zhao
Summary: This study found that the use of 1 μM NMN can effectively reduce the lipid droplet content in bovine oocytes and decrease the expression levels of genes related to lipid synthesis. Vitrified bovine oocytes treated with 1 μM NMN had a higher survival rate and better development ability compared to other treatment groups. Additionally, 1 μM NMN, 2.5 μM BER, and 1 μM COR also decreased the levels of ROS and apoptosis, reduced the mRNA expression levels of genes involved in ER stress and mitochondrial fission, and increased the mRNA expression levels of genes associated with mitochondrial fusion in vitrified bovine oocytes. The results suggest that 1 μM NMN, 2.5 μM BER, and 1 μM COR effectively reduce lipid droplet content and enhance the development ability of vitrified bovine oocytes by regulating ROS levels, ER stress, mitochondrial function, and apoptosis. Furthermore, 1 μM NMN was more effective than 2.5 μM BER and 1 μM COR.
Article
Reproductive Biology
Emily Girka, Lauren Gatenby, Emilio J. Gutierrez, Kenneth R. Bondioli
Summary: This study aimed to improve meiotic spindle recovery in vitrified bovine oocytes by investigating the effects of treatment with a microtubule stabilizing agent or a microtubule recovery agent. The results showed that treatment with paclitaxel at a certain concentration significantly increased the proportion of oocytes with normal microtubule distribution and chromosome arrangement after warming. Additionally, inhibiting rho-associated coiled-coil kinase activity and accumulation of cysteine and cysteamine also improved meiotic spindle recovery in vitrified oocytes.
Article
Anatomy & Morphology
Xiangning Xu, Xiuying Jiang, Ke Song, Jiaqi Zhang, Ying Tian, Ye Chen, Jing Weng, Yuanjing Liang, Wei Ma
Summary: In this study, we investigated the expression and function of Odf2 in oocyte meiosis. We found that Odf2 is stably expressed in mouse oocytes during meiotic progression and is located at multiple sites including microtubule organizing centers, chromosome centromeres, and vesicles. Furthermore, Odf2 co-localizes with the mitochondrial sheath in the sperm tail and remains on vesicles in early embryos. These findings suggest that Odf2 may play a role in regulating oocyte spindle assembly and positioning, as well as sperm motility and early embryo development.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2023)
Article
Biochemistry & Molecular Biology
Xi Xu, Tong Hao, Emma Komba, Baigao Yang, Haisheng Hao, Weihua Du, Huabin Zhu, Hang Zhang, Xueming Zhao
Summary: This study aimed to investigate the effect of vitrification on the abundance and post-translational modifications of JUNO protein in bovine oocytes. The results showed that vitrification did not alter the amino acid sequence of JUNO protein, but induced changes in post-translational modifications and protein abundance. Furthermore, combination treatment of JUNO mRNA microinjection and CLC/M beta CD improved the fertilization and development ability of vitrified bovine oocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Agriculture, Dairy & Animal Science
Shinichi Hochi
Summary: This article provides an up-to-date review of cryodevices suitable for the minimum volume cooling vitrification method of bovine oocytes, and discusses some optimization measures.
ANIMAL SCIENCE JOURNAL
(2022)
Article
Reproductive Biology
Tamas Somfai, Yuji Hirao
Summary: An optimized vitrification protocol using protein-free media was established for the cryopreservation of immature bovine oocytes, which resulted in high blastocyst quality without noticeable toxic effects. Both NCSU-37 and TCM-199 were equally effective as base media for vitrification.
Article
Reproductive Biology
Emilio Gutierrez-Castillo, Fabian A. Diaz, Sydney A. Talbot, Kenneth R. Bondioli
Summary: The present study aimed to investigate the effects of post-warming culture time on the meiotic spindle and mitochondrial function of vitrified bovine oocytes. The results showed that extended culture promoted the recovery of the meiotic spindle, while the recovery of mitochondrial function varied.
Article
Biochemistry & Molecular Biology
Neda Abedpour, Hamed Shoorei, Farzad Rajaei
Summary: This study aimed to evaluate the effects of vitrification on fertilization rate and expression of integrin genes in mice oocytes. The results showed that vitrified oocytes had a lower fertilization rate and altered expression of integrin genes compared to non-vitrified oocytes.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biology
Yuji Endo, Yoshitaka Fujii, Hiroaki Motoyama
Summary: This study compared the efficiency of two non-biological devices (Cryotop and Cell Sleeper) in cryopreserving small numbers of human spermatozoa. The results showed that the Cryotop group had higher post-warmed sperm motility and fertilization rates after intra-cytoplasmic sperm injection. Both devices have clinical advantages in terms of easy use and safety, and can be used more efficiently with devices of different properties.
Review
Endocrinology & Metabolism
Xiao Fu, Yiting Zhang, Shuli Gao, Shuzhe Gao, Meng Zhang, Shanshan Gao, Jinlong Ma, Zi-Jiang Chen
Summary: Different indications for oocyte vitrification do not affect clinical pregnancy rates. The clinical pregnancy rate of vitrified oocytes for different indications is correlated with age and number of thawed oocytes. For women with an abundant number of retrieved oocytes, cryopreserving a small number of oocytes is a valuable option that may benefit them in the future. Additional studies with large-scale, variable-controlled methodologies and extended follow-up are needed to further validate and clarify these findings.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Thatawat Yodrug, Rangsun Parnpai, Yuji Hirao, Tamas Somfai
Summary: Vitrification of bovine oocytes at different meiotic stages affects blastocyst development after IVF, with GV-stage vitrification showing higher developmental success than MII-stage. DNA methylation alterations were observed in blastocysts, but not in oocytes. Histone acetylation and methylation were not influenced by oocyte vitrification.
ANIMAL SCIENCE JOURNAL
(2021)
Article
Reproductive Biology
Maria Florencia Suqueli Garcia, Nadia Gabbanelli, Glenda Laura Rios, Jorgelina Buschiazzo
Summary: This study found that progesterone (P4) can induce acrosome exocytosis in sperm without compromising sperm viability or activating sperm caspases. Furthermore, using lower concentrations of exogenous P4 during in vitro fertilization (IVF) can enhance the developmental competence of partially cumulus-denuded oocytes.
Article
Genetics & Heredity
Yasuhiro Kazuki, Feng J. Gao, Miho Yamakawa, Masumi Hirabayashi, Kanako Kazuki, Naoyo Kajitani, Sachiko Miyagawa-Tomita, Satoshi Abe, Makoto Sanbo, Hiromasa Hara, Hiroshi Kuniishi, Satoshi Ichisaka, Yoshio Hata, Moeka Koshima, Haruka Takayama, Shoko Takehara, Yuji Nakayama, Masaharu Hiratsuka, Yuichi Iida, Satoko Matsukura, Naohiro Noda, Yicong Li, Anna J. Moyer, Bei Cheng, Nandini Singh, Joan T. Richtsmeier, Mitsuo Oshimura, Roger H. Reeves
Summary: A transchromosomic rat model of Down syndrome (DS) was developed, showing DS-related characteristics such as learning and memory deficits, increased anxiety and hyperactivity, and reproducing well-characterized brain morphology, cerebellar size reduction, and anomalies in craniofacial morphology. This robust DS animal model can facilitate DS research and preclinical validation for drug development.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Review
Agriculture, Dairy & Animal Science
Shinichi Hochi
Summary: This article provides an up-to-date review of cryodevices suitable for the minimum volume cooling vitrification method of bovine oocytes, and discusses some optimization measures.
ANIMAL SCIENCE JOURNAL
(2022)
Article
Endocrinology & Metabolism
Jing Chen, Shiori Minabe, Arisa Munetomo, Fumie Magata, Marimo Sato, Sho Nakamura, Masumi Hirabayashi, Yasuhiro Ishihara, Takeshi Yamazaki, Yoshihisa Uenoyama, Hiroko Tsukamura, Fuko Matsuda
Summary: The postnatal increase in testosterone in male rats is mainly induced by central kisspeptin-dependent stimulation of GnRH and consequent LH release.
Article
Biochemistry & Molecular Biology
Mami Oikawa, Mayuko Nagae, Naoaki Mizuno, Kenyu Iwatsuki, Fumika Yoshida, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura, Hiromitsu Nakauchi, Masumi Hirabayashi, Toshihiro Kobayashi
Summary: In this study, various gene targeting strategies were compared to generate a novel knock-in reporter rat line. By visualizing the expression pattern of the transcription factor Tfap2c, this rat line can be used to identify and purify cells expressing Tfap2c in rats, making it a valuable resource.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Teppei Goto, Kyoko Yogo, Shinichi Hochi, Masumi Hirabayashi
Summary: This study compared the efficiency of generating specific gene mutations in embryos through pronuclear injection of Cas9 in the form of protein, mRNA or plasmid DNA. Results showed that Cas9 protein in the pronuclear injection could efficiently produce homozygous mutant blastocysts. Therefore, Cas9 protein form has significant application value in gene editing.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Jinsoo Ahn, In-Sul Hwang, Mi-Ryung Park, Seongsoo Hwang, Kichoon Lee
Summary: Our comparative study revealed the imprinted expression of the KBTBD6 gene in domesticated mammals, but not in humans, non-human primates, and mice, implicating the species-specific evolution of genomic imprinting.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Wen-Jie Jiang, Song-Hee Lee, Geun Heo, Hak Jae Chung, Eun Seok Cho, Soo Jin Sa, Shinichi Hochi, Xiang-Shun Cui
Summary: YBX1 plays important roles in the development of porcine preimplantation embryos, and its knockdown leads to decreased mitochondrial function, increased ER stress, and enhanced autophagy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemical Research Methods
Kenyu Iwatsuki, Mami Oikawa, Hisato Kobayashi, Christopher A. Penfold, Makoto Sanbo, Takuya Yamamoto, Shinichi Hochi, Kazuki Kurimoto, Masumi Hirabayashi, Toshihiro Kobayashi
Summary: This article investigates the role of pluripotent stem cells (PSCs) in lineage specification and demonstrates the ability to reset rat EpiSCs and their competency for germ cell development, uncovering principles underlying pluripotency and germline competency.
CELL REPORTS METHODS
(2023)
Article
Agriculture, Dairy & Animal Science
Yong-Min Kim, Sung-Woo Park, Mi-Jin Lee, Da-Yeon Jeon, Su-Jin Sa, Yong-Dae Jeong, Ha-Seung Seong, Jung -Woo Choi, Shinichi Hochi, Eun-Seok Cho, Hak-Jae Chung
Summary: In this study, the iTRAQ technique and LC-MS/MS analysis were used to identify differentially expressed CM10-fractionated proteins in fresh and frozen-thawed boar semen. The results showed that 9 proteins were upregulated and 67 proteins were downregulated in frozen-thawed samples. These proteins are involved in oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mi-Ryung Park, Jin Seop Ahn, Min Gook Lee, Bo Ram Lee, Sun A. Ock, Sung June Byun, In-Sul Hwang
Summary: This study identifies the characteristics of enlarged tongues in cloned piglets through histological and transcriptomic analysis. The analysis reveals isolated and broken muscle bundles with wide spaces in enlarged tongues, as well as differential gene expression related to the muscle system process and glycolytic regulation. These findings provide insights into the abnormal development and disease diagnosis in cloned pigs.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kang Won Park, Hyeon Yang, Min Gook Lee, Sun A. Ock, Hayeon Wi, Poongyeon Lee, In -Sul Hwang, Jae Gyu Yoo, Choon-Keun Park, Bo Ram Lee
Summary: This study successfully established intestinal organoids derived from growing cattle and generated apical-out intestinal organoids. These organoids exhibited key functionality, such as superior permeability. They could serve as valuable tools and potential alternatives for studying host-pathogen interactions.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Shinichi Hochi, Misuzu Ide, Sayaka Ueno, Masumi Hirabayashi
Summary: This study compared the efficiency of producing bovine blastocysts by ICSI and IVF from vitrified-warmed and fresh oocytes. It found that ICSI had a higher blastocyst yield in vitrified-warmed oocytes compared to IVF.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Endocrinology & Metabolism
Jing Chen, Shiori Minabe, Arisa Munetomo, Fumie Magata, Marimo Sato, Sho Nakamura, Masumi Hirabayashi, Yasuhiro Ishihara, Takeshi Yamazaki, Yoshihisa Uenoyama, Hiroko Tsukamura, Fuko Matsuda
Summary: This study aimed to investigate the role of Kiss1/kisspeptin in generating perinatal plasma LH and the consequent testosterone increase in male rats. The results showed that Kiss1 knockout male rats had significantly lower postnatal plasma testosterone and LH levels compared to wild-type male rats, while the levels during the prenatal period were similar between the genotypes. Exogenous kisspeptin stimulation increased postnatal plasma testosterone and LH levels in both Kiss1 knockout and wild-type male rats, indicating that the postnatal testosterone increase in male rats is mainly regulated by central kisspeptin.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
