Article
Agriculture, Dairy & Animal Science
Xiaona Chen, Xinyu Duan, Qingqing Chong, Chunqing Li, Heng Xiao, Shanyuan Chen
Summary: This study analyzed the differences in DNA methylation between zebu and taurine cattle and identified pathways and candidate genes associated with disease, disease resistance, and economic traits. These findings provide a foundation for further screening of characteristic epigenetic molecular markers in zebu and support genetic improvement of domestic cattle.
Article
Agriculture, Dairy & Animal Science
Maulana M. Naji, Yuri T. Utsunomiya, Johann Solkner, Benjamin D. Rosen, Gabor Meszaros
Summary: This study compared the alignment of sequence data from different breeds of cattle to two different reference genomes, revealing that aligning to UOA_Brahman_1 detected more SNVs, especially in cross-subspecies alignments. Unexpectedly low SNV counts in certain regions suggest the presence of taurine DNA in the UOA_Brahman_1 reference genome. Analysis of taurine-introgressed loci using principal component and admixture analyses confirmed the presence of these loci.
GENETICS SELECTION EVOLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Jisung Jang, Kwondo Kim, Young Ho Lee, Heebal Kim
Summary: This study called and compared autosome-wide CNVs in Eurasian taurine, Asian indicine, African taurine, and African humped cattle using NGS data, revealing significant population differentiated CNVRs and genes that may be under selection, including those related to various traits. Additionally, breed- and individual-level comparisons of CNVR presence or copy number showed important CNVs potentially influencing cattle adaptation to different environments, based on the largest dataset of 336 individuals and 39 breeds analyzed with short Illumina reads.
Article
Agriculture, Dairy & Animal Science
Y. T. Utsunomiya, A. A. A. D. Fortunato, M. Milanesi, B. B. Trigo, N. F. Alves, T. S. Sonstegard, J. F. Garcia
Summary: Brazil is the largest exporter of beef in the world, with most of its beef derived from Nellore cattle. The history of Nellore cattle in Brazil involves importation of bulls from India, leading to genetic influences from both zebu and taurine breeds. Despite the small average taurine ancestry of approximately 1%, some taurine variants significantly impact traits such as body size and horn characteristics in Nellore cattle.
Article
Biology
Bosco Jose, Hari Abdul Samad, Jaya Bharati, V. Tejaswi, Pranay Konda, Khan Sharun, Manoj K. Tripathi, Sai Kumar, Meeti Punetha, Divya Mohan, V. Verma, Vikrant Singh Chouhan, V. P. Maurya, G. Taru Sharma, Puneet Kumar, Mihir Sarkar, Gyanendra Singh
Summary: Global warming has made livestock more susceptible to heat stress, threatening the sustainability of marginal farmers. This study compared the thermo-adaptability of Tharparkar calves, an indigenous breed, and crossbred calves during heat stress. The results showed that Tharparkar calves exhibited better thermo-adaptability than crossbred calves in different heat stress conditions.
JOURNAL OF THERMAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Juliana Ranches, Rhaiza Alves, Marcelo Vedovatto, Elizabeth A. Palmer, Philipe Moriel, John D. Arthington
Summary: This study observed significant differences in the metabolism of copper and selenium between Angus and Brahman cattle, including concentration in the liver and variations at different stages.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agronomy
Qianqian Zhang, Anna Amanda Schonherz, Mogens Sando Lund, Bernt Guldbrandtsen
Summary: This study demonstrates the combined effects of positive selection and adaptive introgression on the modern bovine genome through in-depth assessment of sequence variation on a whole-genome scale. The observed hotspots of runs of homozygosity (ROH) haplotypes were confirmed to be results of positive selection and were associated with productive traits in modern Bos taurus cattle breeds. The study also identifies the introgression events of haplotypes from Bos indicus to Bos taurus, contributing to the formation of the current bovine genome.
Article
Agriculture, Dairy & Animal Science
Rebeka Sinha, Beena Sinha, Ragini Kumari, M. R. Vineeth, Nisha Sharma, Archana Verma, Ishwar Dayal Gupta
Summary: This study measured udder traits in different breeds of cows and investigated the association between these traits and genetic polymorphisms. The results showed that specific genotypes were associated with udder characteristics in different breeds of cows.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Narian Romanello, Andrea do Nascimento Barreto, Marco Antonio Paula de Sousa, Julio Cesar de Carvalho Balieiro, Felipe Zandonadi Brandao, Felipe Tonato, Alberto Carlos de Campos Bernardi, Jose Ricardo Macedo Pezzopane, Jose Alberto Bastos Portugal, Alexandre Rossetto Garcia
Summary: This study evaluated the microclimate and thermoregulatory and endocrine responses of zebu and composite bulls in a non-shaded pasture system (NS) and an integrated crop-livestock-forestry system (ICLF). The results showed that the ICLF system can reduce air temperature, Black Globe Temperature and Humidity Index (BGHI), and Radiant Thermal Load, providing better thermal comfort and homeothermy for the cattle.
AGRICULTURAL SYSTEMS
(2023)
Article
Biology
Mehrnush Forutan, Elizabeth Ross, Amanda J. Chamberlain, Loan Nguyen, Brett Mason, Stephen Moore, Josie B. Garner, Ruidong Xiang, Ben J. Hayes
Summary: By profiling genome-wide transcriptional start sites (TSSs) in two subspecies of cattle, researchers identified evolutionary and developmental differences in TSSs, with some SNPs located in differentially used TSS clusters showing significant shifts in allele frequency. Additionally, higher relative TSS expression of heat shock protein genes was observed in Bos indicus compared to Bos taurus, which may contribute to the tropical adaptation of Bos indicus. This study provides insights into cattle adaptation and highlights the rapid evolution of TSS between recently diverged subspecies.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Evgenia Solodneva, Gulnara Svishcheva, Rodion Smolnikov, Sergey Bazhenov, Evgenii Konorov, Vera Mukhina, Yurii Stolpovsky
Summary: Every week, 1-2 breeds of farm animals, including local cattle, disappear in the world. As the keepers of rare allelic variants, native breeds potentially expand the range of genetic solutions to possible problems of the future, which means that the study of the genetic structure of these breeds is an urgent task. Providing nomadic herders with valuable resources necessary for life, domestic yaks have also become an important object of study.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Gustavo Andre Bernado Moura, Cintia Carol de Melo Costa, Vinicius de Franca Carvalho Fonseca, Gene Wijffels, Patric Andre Castro, Marcos Chiquitelli Neto, Alex Sandro Campos Maia
Summary: In moderate thermal conditions, crossbred cattle showed higher levels of heat production, thermal storage, and cutaneous evaporation compared to purebred Nellore cattle. Both genotypes were able to maintain their thermal balance with relatively low energy demand for thermoregulation.
Review
Cell Biology
Arumugam Kumaresan, Kamaraj Elango, Tirtha Kumar Datta, Jane M. Morrell
Summary: Crossbreeding of indigenous cattle with improved breeds has led to higher milk production, but the resulting crossbred offspring face significant reproductive problems. Research efforts have focused on identifying the reasons behind infertility in crossbred bulls through various molecular analyses, but a clear understanding of the underlying causes is still lacking. Thorough studies are needed to address these challenges and pave the way for future research on improving crossbred male fertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Larissa Zamparone Bergamo, Denis Vinicius Bonato, Camila Bizarro-Silva, Francieli Gesleine Capote Bonato, Suellen Miguez Gonzalez, Ana Carolina Rossaneis, Waldiceu A. Verri Jr, Fabio Morotti, Marcelo Marcondes Seneda
Summary: This study found that the addition of alpha-lipoic acid in the in vitro culture medium can promote follicular development and maintain morphological integrity in preantral ovarian follicles from Bos taurus indicus females. It can also help maintain stable levels of reactive oxygen species.
Article
Agriculture, Dairy & Animal Science
Devesh Kumar Yadav, Zile S. S. Malik, Spandan Shashwat Dash, Yogesh C. Pushpa, Yogesh C. Bangar, Ankit Magotra
Summary: The aim of this study was to analyze the risk and genetic effects on calf survival in Hardhenu cattle using data records of 2593 calves born to 102 sires and 790 dams over 25 years. Bayesian analysis was employed to estimate direct and maternal effects on animal survival. The results showed higher mortality rates for male animals and calves born during the rainy season. The inclusion of survival traits in selection criteria may improve calf survival and economic gain in the dairy herd.
JOURNAL OF ANIMAL BREEDING AND GENETICS
(2023)
Article
Reproductive Biology
D. V. Bonato, E. B. Ferreira, D. N. Gomes, F. G. C. Bonato, R. G. Droher, F. Morotti, M. M. Seneda
Summary: Two experiments were conducted in high milk-producing Holstein cows to evaluate the repeatability of antral follicle count (AFC) during ovulation synchronization protocol and investigate the follicular dynamics and Doppler ultrasonography. The results showed that AFC has high repeatability and cows with low AFC exhibited larger preovulatory follicles, greater perfusion in corpus luteum, and higher progesterone levels.
Article
Veterinary Sciences
S. B. Delchiaro, D. Bonato, P. V. L. F. Oliveira, R. J. Paulossi Junior, F. G. C. Bonato, M. M. Seneda, F. Morotti
Summary: This study investigated the prepubertal characteristics of Nelore and crossbred Nelore × Aberdeen Angus heifers. The results showed that regardless of hormone induction, the count of antral follicles was highly repeatable in both breeds during puberty induction. The crossbred heifers exhibited higher body condition score, average weight gain, and diameter of dominant follicles, indicating greater precocity compared to Nelore heifers.
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2022)
Article
Veterinary Sciences
J. R. Silva, C. F. Medeiros, M. B. Souza-Caceres, J. M. Gheller, W. A. L. Silva, J. V. Santos, M. G. C. R. Ferreira, A. L. J. Ferraz, F. A. Melo-Sterza
Summary: This study evaluated the relationship between AFC and ovarian morphology in Bos indicus cows of different age groups. The results showed that AFC was most closely correlated with the weight of small follicles, and there was no relationship between AFC and the weight and diameter of dominant follicles and corpus luteum. The study concludes that AFC can be performed on a single ovary regardless of the presence of dominant follicles and corpus luteum.
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2022)
Article
Agriculture, Multidisciplinary
Emanuel Binotto Ferreira, Gustavo Martins Gomes dos Santos, Sofia Botsaris Delchiaro, Tamires Korchovei Sanches, Fabio Morotti, Marcelo Marcondes Seneda
Summary: This study investigated the effects of a homeopathic product on estrus manifestation, ovulation, and blood flow in Nelore x Aberdeen Angus heifers, and found no significant difference compared to the control group.
SEMINA-CIENCIAS AGRARIAS
(2022)
Article
Cell Biology
Suellen Miguez Gonzalez, Marcela Bortoletto Cerezetti, Larissa Zamparone Bergamo, Fabio Morotti, Marcelo Marcondes Seneda
Summary: The aim of this study was to investigate the distribution of preantral follicles in bovine ovaries. The ovaries from Nelore breed heifers were evaluated in two different regions. The region close to the ovarian pedicle (OP) showed a higher number of preantral follicles compared to the region of the greater curvature of the ovary (GCO). These findings indicate that the distribution of preantral follicles in bovine ovaries is heterogeneous, with a higher concentration near the OP region.
Review
Agriculture, Dairy & Animal Science
Camila Bortoliero Costa, Trudee Fair, Marcelo M. Seneda
Summary: The oocyte is crucial for the development of life and plays a significant role in embryonic survival and successful pregnancy. This article focuses on key aspects of oocyte and follicle development in cattle, as well as recent advancements in reproductive biotechnologies centered around the oocyte and follicle.
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anne Kemmer Souza, Amanda Fonseca Zangirolamo, Ricardo Guella Droher, Francieli Gesleine Capote Bonato, Amauri A. Alfieri, Marcio Carvalho da Costa, Marcelo Marcondes Seneda
Summary: This study characterized the bovine vaginal microbiota using long-read sequencing (PacBio) and compared it with short-read sequencing (Illumina). PacBio sequencing detected more unique and rare species, while Illumina sequencing detected more observed species. The composition of the vaginal microbiota varies according to the sequencing method used.
Article
Agriculture, Dairy & Animal Science
Wilian Aparecido Leite da Silva, Eliane Vianna da Costa e Silva, Mariane Gabriely Cesar Ribeiro Ferreira, Janaina Menegazzo Gheller, Ralf Poehland, Fabiana de Andrade Melo-Sterza
Summary: This study evaluated the physiological and reproductive parameters of Nelore cows under different microclimatic conditions. The results showed that cows with shade had lower body temperatures but it did not affect conception rate and pregnancy loss.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Agriculture, Multidisciplinary
Janaina Menegazzo Gheller, Geancarlos Carraro da Silva, Wilian Aparecido Leite da Silva, Aldair Felix da Silva, Luiz Carlos Cesar Costa Filho, Eriklis Nogueira, Fabiana de Andrade Melo-Sterza
Summary: This study evaluated the ovarian structure, estrus intensity, ultrasound carcass measurements, and pregnancy rate of Nelore breed heifers and cows based on antral follicle counts (AFCs). The results showed that Nelore heifers and cows with low AFC had a high probability of pregnancy during the entire breeding season, and AFC can be used as a tool to select heifers with increased fertility without affecting carcass traits.
SEMINA-CIENCIAS AGRARIAS
(2023)
Article
Agriculture, Dairy & Animal Science
Suellen Miguez Gonzalez, Marcela Bortoletto Cerezetti, Larissa Zamparone Bergamo, Camila Rodrigues Ferraz, Waldiceu Aparecido Verri Jr, Marcelo Marcondes Seneda
Summary: The aim of this study was to evaluate oxidative stress in ovaries and corpus luteum (CL) of Bos taurus indicus females and the oxidant effect of CL in ovarian tissues. The results showed that luteal tissues had higher antioxidant activity than ovarian tissues, and there was no difference in antioxidant activity and oxidative stress between ovarian fragments obtained from different regions (with or without CL). The presence of CL in the ovaries did not influence oxidative stress or antioxidant potential.
ANIMAL REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
Aricia Gomes Miranda, Marcelo Marcondes Seneda, Luciana Rocha Faustino
Summary: This study reviewed the etiology of polycystic ovary syndrome (PCOS) and the influence of DNA methylation on the main genes involved in PCOS. It found that INSR, LHCGR, and RAB5B genes may undergo epigenetic alterations in DNA, with the first two genes being hypomethylated and the last one being hypermethylated.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Agriculture, Multidisciplinary
Luiz Francisco Machado Pfeifer, Jair Sabio de Oliveira Junior, Marcelo Marcondes Seneda, Higor Souza de Camargo, Fabio Morotti, Evelyn Rabelo Andrade, Elizangela Mirian Moreira, Jessica Souza Andrade, George Moreira da Silva, Marlos Oliveira Porto, Jucilene Cavali
Summary: The objective of this study was to investigate the effects of puberty and parturition on antral follicle count (AFC) in Nelore beef cattle. The results showed that AFC and follicle concentrations did not differ between the prepubertal and pubertal periods, but the ovarian area increased after puberty. Additionally, AFC and ovarian area were greater in the postpartum period compared to the prepubertal period.
PESQUISA AGROPECUARIA BRASILEIRA
(2023)
Article
Agriculture, Dairy & Animal Science
Fabio Lucas Zito de Moraes, Denis Vinicius Bonato, Ana Clara Canto Souza, Fabio Morotti, Marcelo Marcondes Seneda
Summary: This study evaluated the relationship between AFC and oestrus expression in beef cows receiving TAI, and found that AFC and oestrus expression classification influenced the conception rate of Nelore cows in the TAI programme. Especially, cows with low AFC and high-intensity oestrus expression had the highest conception rate.
ANIMAL PRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Fabio Morotti, Suellen Miguez-Gonzalez, Marcela Bortoletto Cerezetti, Marcelo Marcondes Seneda
Summary: This study evaluated different classification methods for antral follicle count (AFC) in cows and found that regardless of the method used, cows with low AFC had higher conception rates and larger dominant follicles during timed artificial insemination (TAI).
ANIMAL REPRODUCTION
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
