Article
Veterinary Sciences
Kate E. Hartzler, Chiara McCartney, Nucharin Songsasen, Jennifer B. Nagashima
Summary: Techniques for the preservation and use of gonadal tissues are crucial for genetic management of the endangered African painted dog. This study evaluated two cryopreservation techniques for ovarian tissue and found needle immersed vitrification to be more effective in maintaining the density of morphologically normal follicles compared to slow freezing protocols. Future research should focus on developing improved methods for ovarian tissue culture to assess the efficacy of cryopreservation techniques and produce viable oocytes from banked ovarian tissue in this endangered species.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Eric M. Gese, Patricia A. Terletzky, Cole A. Bleke, Erika T. Stevenson, Susannah S. French
Summary: This study examined the stress levels of captive coyotes during breeding and pup production using fecal sampling. The results showed that producing pups did not significantly impact the stress levels in coyotes.
Article
Veterinary Sciences
Laurie Marker, Meredith Honig, Lauren Pfeiffer, Monique Kuypers, Kathy Gervais
Summary: African wild dogs are endangered due to habitat fragmentation and human activities, highlighting the importance of conservation efforts. The successful rescue and rearing of orphaned pups by the Namibian Ministry of Environment demonstrates the potential for rehabilitation and eventual release back into the wild, providing valuable insights into the care and growth of these vulnerable animals.
Article
Reproductive Biology
Cristina Gobello
Summary: Canine pseudocyesis, also known as false pregnancy, is a common syndrome observed in non-pregnant female dogs, characterized by mammary gland enlargement, maternal behavior, and lactation. Further education is necessary to ensure optimal diagnosis and treatment strategies for this physiological event.
Article
Agriculture, Dairy & Animal Science
Leila Siciliano-Martina, Jason P. Martina, Emma Dohnalik, Grace Vielleux
Summary: Maintaining reproductive seasonality is crucial for wild animals, but in captivity, certain species may reproduce throughout the year and produce multiple litters annually. We studied the reproductive variability of captive-born fennec foxes and found that litters were born during all months of the year, with almost half born outside of the expected months. Many foxes produced multiple litters in a year, and factors such as habituation to the birth location, age, previous litter number, and birth month of the dam influenced multi-litter years. Although there were anecdotal claims linking multi-litter years to the loss of previous litters, we did not find any evidence supporting this. These findings suggest that captive fennec fox populations may experience disrupted reproductive patterns, leading to the production of litters outside of the typical reproduction window. Understanding their reproduction can improve captive management and future breeding success.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Ekaterina Semenova, Mariusz P. Grudniak, Katarzyna Bocian, Magdalena Chroscinska-Krawczyk, Marzena Trochonowicz, Igor M. Stepaniec, Magdalena Murzyn, Ilona Szablowska-Gadomska, Dariusz Boruczkowski, Tomasz Oldak, Eugeniusz K. Machaj
Summary: The processing of MSCs involves in vitro expansion and freezing storage, with an emphasis on optimizing the freezing process to retain physiological properties. Research shows that using an appropriate DMSO-based cryoprotectant mixture can effectively preserve the physiological properties of AT-MSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Lachlan G. Howell, Stephen D. Johnston, Justine K. O'Brien, Richard Frankham, John C. Rodger, Shelby A. Ryan, Chad T. Beranek, John Clulow, Donald S. Hudson, Ryan R. Witt
Summary: Managed wildlife breeding faces challenges related to high costs and genetic diversity. Biobanking and assisted reproductive technologies can help address these issues by enhancing genetic diversity and reducing program costs. Integrating biobanking into Australia's zoo and wildlife hospital network provides a feasible model for koala conservation.
Article
Multidisciplinary Sciences
Emily J. Howells, Mary Hagedorn, Madeleine J. H. Van Oppen, John A. Burt
Summary: Reef-building corals, living near their upper thermal limits, are endangered by global warming. Cross-breeding with heat-adapted populations can potentially increase the thermal limits of sensitive corals, but the regional variation in their reproductive cycles poses limitations. This study overcame this barrier by using cryopreservation technology to cross-breed conspecific coral populations across ocean basins for the first time. The results highlighted the challenges and potential approaches for breeding corals with enhanced thermal tolerance.
Article
Microbiology
Wenjing Jiao, Lina Liu, Zhiliao Zeng, Linmiao Li, Jinping Chen
Summary: Intestinal microorganisms are crucial for the health of pangolins, with adult Sunda pangolins possessing more diversity and proportion of microbial species to resist environmental pressure.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Reproductive Biology
Leonardo L. Lyrio, Monique A. Lazaro, Raphael Sonegheti, Luiza Moulin, Lara Coslop, Cibely Galvani Sarto, Barbara Loureiro, Mauricio G. Favoreto
Summary: This study aimed to evaluate the effects of heat stress on French Bulldogs' semen quality and testicular artery characteristics. The results showed that heat stress negatively impacted sperm quality, but the effects were temporary.
Article
Biodiversity Conservation
Lachlan G. Howell, Richard Frankham, John C. Rodger, Ryan R. Witt, Simon Clulow, Rose M. O. Upton, John Clulow
Summary: Captive breeding is an important part of global conservation efforts, but integrating biobanking of founder sperm can greatly reduce costs and achieve long-term genetic diversity retention targets. By using frozen sperm for backcrossing, the study showed significant reductions in expenditure and the potential for producing more animals of higher genetic quality through assisted breeding. This innovation could enhance credibility and support for captive breeding as a conservation strategy.
CONSERVATION LETTERS
(2021)
Article
Veterinary Sciences
Mohamed A. A. Mahdy, Walid Fathy Mohamed
Summary: This study compared the craniometric measurements of Egyptian red fox and Baladi dog skulls and found significant differences in size and shape between the two species. Certain measurements also showed that the domestic dog had superior values compared to the red fox, providing valuable information for ecological studies, comparative anatomy, and veterinary sciences.
BMC VETERINARY RESEARCH
(2022)
Review
Agriculture, Dairy & Animal Science
Feriel Yasmine Mahiddine, Min-Jung Kim
Summary: The article reviews the antioxidants, egg yolk alternatives, and MSCs used in canine sperm cryopreservation. Sperm cryopreservation is a widely used assisted reproductive technology for dogs, effective for long-distance breeding and disease prevention in the reproductive tract.
Article
Marine & Freshwater Biology
Jonathan Daly, Rebecca J. Hobbs, Nikolas Zuchowicz, Justine K. O'Brien, Jessica Bouwmeester, Line Bay, Kate Quigley, Mary Hagedorn
Summary: Maintaining genetic diversity and biodiversity is crucial for the health and adaptability of ecosystems. Assisted Gene Flow (AGF) is a management approach that involves transferring adaptive genes between populations to restore declining and at-risk habitats. This study demonstrates that cryopreserved sperm can be used for intrapopulation and interpopulation crosses to support AGF. The concentration of motile sperm in post-thaw samples is an important factor for successful fertilization. Coral reefs with high species and genetic diversity and coral cover are suitable for preserving biodiversity through cryopreservation.
Article
Developmental Biology
Lachlan G. Howell, Richard Frankham, John C. Rodger, Ryan R. Witt, Simon Clulow, Rose M. O. Upton, John Clulow, Peter R. Mawson, Natalie E. Calatayud
Summary: Captive breeding is crucial for amphibian conservation, and integrating biobanking and assisted reproductive technologies can help overcome the negative impacts of sustained captivity. Modelled captive populations of threatened Australian frogs demonstrate the effectiveness of this integration in minimizing genetic risks and reducing program costs.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.