Article
Endocrinology & Metabolism
Stine Gry Kristensen, Ajay Kumar, Linn Salto Mamsen, Bhanu Kalra, Susanne Elisabeth Pors, Jane Alro Botkjaer, Kirsten Tryde Macklon, Jens Fedder, Erik Ernst, Kate Hardy, Stephen Franks, Claus Yding Andersen
Summary: This study investigated the differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in follicle fluids from women with and without polycystic ovaries (PCO). The results showed that GDF9 levels were significantly higher in women with PCO, while BMP15 levels were significantly lower, resulting in an increased GDF9/BMP15 ratio. This aberrant endocrine environment may serve as a new biomarker for PCO.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Obstetrics & Gynecology
Bruce S. Shapiro, Melody A. Rasouli, Kajal Verma, Ankita Raman, Forest C. Garner, Martha Aguirre, Leah Kaye, Carrie Bedient
Summary: This study aimed to examine the relationship between the size of punctured ovarian follicles and embryology outcomes. The study found that punctures of follicles smaller than 12.5 mm rarely resulted in good-quality blastocysts, while punctures of follicles in the 19-24.5 mm range were associated with higher yields of good-quality blastocysts than the average. Other groups did not show significant differences from the average, and there was no correlation between follicle size and the ploidy of biopsied blastocysts.
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
Aiat Shamsa, Robert B. Gilchrist, David M. Robertson, Rachael J. Rodgers, Mark W. Donoghoe, William L. Ledger, Jason A. Abbott, Angelique H. Riepsamen
Summary: This study investigated the impact of endometriosis on the serum concentrations of oocyte-secreted factors, GDF9 and BMP15. The results of a case-control study on 60 women showed that endometriosis does not affect the levels of GDF9 and BMP15 in the serum.
REPRODUCTIVE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Jesus Cadenas, Susanne Elisabeth Pors, Ajay Kumar, Bhanu Kalra, Stine Gry Kristensen, Claus Yding Andersen, Linn Salto Mamsen
Summary: This study found that native pro-mature GDF9 and BMP15 proteins exist in human oocytes. The concentrations of GDF9 and BMP15 change during in vitro maturation, with GDF9 being approximately 10 times higher in concentration than BMP15. The role of the GDF9/BMP15 heterodimer in oocyte development is still uncertain.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Mishuk Shaha, Gous Miah, Arjuman Lima, Omar Faruk Miazi, Mukta Das, Ashutosh Das
Summary: The associations between polymorphisms in the GDF9 and BMP15 genes and litter size were investigated in Jamunapari and crossbred goats in Bangladesh. Four SNPs in GDF9 and three SNPs in BMP15 were identified. The G1330T locus was found to have a significant difference in litter size in Jamunapari goats. This finding suggests that the G1330T locus could be used as a marker for litter size traits in goats in Bangladesh.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2022)
Article
Reproductive Biology
Bethany K. Redel, Lee D. Spate, Ye Yuan, Clifton N. Murphy, R. Michael Roberts, Randall S. Prather
Summary: During in-vitro maturation of immature porcine oocytes, gonadotropins are not necessary when FGF2, LIF, and IGF1 are present. FLI directly impacts oocyte competence without the need for gonadotropins, and in the presence of gonadotropins, it enhances cumulus cell expansion.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Aftab Ullah, Rajwali Khan, Syed Muhammad Suhail, Ijaz Ahmad, Farhan Anwar Khan, Muhammad Subhan Qureshi, Nazir Ahmad Khan, Amel Ayari-Akkari, Dalia Abd El Moneim Ahmed
Summary: This study investigated the GDF9 gene in Damani goats and identified three novel SNPs in the promoter region that significantly impacted litter size, milk production, and body measurement traits. These SNPs also caused the loss and gain of transcription factor binding sites.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Risu Na, Yan Zeng, Yanguo Han, Chengli Liu, Baigao Yang, Yongmeng He
Summary: The mechanism of multiple ovulations in prolific goats remains unclear, but this study found that nine miRNAs were differently expressed between ovulatory and subordinate follicles in Dazu black goats. Chi-miR-130b-5p and chi-miR-214-3p were predicted to regulate genes involved in gonadotropin hormone signaling and oocyte-derived growth factors. Further studies are needed to confirm these findings.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Jingwei Yang, Jing Gao, Yuting Wang, Hongya Liu, Xuemei Lian
Summary: This study investigated the impact of follicle sizes on oocyte and embryo quality in young and advanced-age patients, finding that triggering when there is a high proportion of large or medium follicles leads to better oocyte quality for young patients, while triggering when the proportion of medium follicles is no less than that of small follicles is more beneficial for older patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Luke Currin, Hernan Baldassarre, Mariana Priotto de Macedo, Werner Giehl Glanzner, Karina Gutierrez, Katerina Lazaris, Vanessa Guay, Maria Elena Carrillo Herrera, Zigomar da Silva, Caitlin Brown, Erin Joron, Ron Herron, Vilceu Bordignon
Summary: The study revealed limited impacts of season and age on the production of water buffalo embryos, but significant variations between individual donors and sires. Additionally, factors like the number of embryos and the preservation method did not significantly affect pregnancy rates after transfer.
Article
Reproductive Biology
Jing Xu, Mary B. Zelinski
Summary: In vitro follicle development (IVFD) is a valuable model for understanding folliculogenesis, screening ovarian toxicity, and preserving fertility and species. This review discusses the culture systems used in IVFD across various species and the improvements made in follicle growth and oocyte maturation through optimization of culture conditions. The evaluation of oocyte quality following IVFD, including epigenetic and genetic profile, maturation, embryonic development, and pregnancy outcomes, is summarized. The review also highlights the limitations and knowledge gaps in IVFD for proper oocyte development. This information contributes to the goal of producing competent oocytes with genetic integrity for successful embryonic development and healthy offspring.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Krishna Chaitanya Pavani, Tim Meese, Osvaldo Bogado Pascottini, Xuefeng Guan, Xiaoyuan Lin, Luc Peelman, Joachim Hamacher, Filip Van Nieuwerburgh, Dieter Deforce, Annekatrien Boel, Bjorn Heindryckx, Kelly Tilleman, Ann Van Soom, Bart M. Gadella, An Hendrix, Katrien Smits
Summary: The miRNA bta-miR-378a-3p in extracellular vesicles plays a crucial role in blastocyst hatching in bovine embryos, improving blastocyst quality and regulating embryo hatching.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Tianzeng Song, Yacheng Liu, Renqing Cuomu, Yao Tan, Cuoji A. Wang, Ji De, Xiaohan Cao, Xianyin Zeng
Summary: In this study, four polymorphic loci were found in specific amplification fragments of BMP15 and GDF9 genes in Tibetan cashmere goats. The mutations G732A and C805G were discovered in the BMP15 gene, while C719T and G1189A mutations were detected in the GDF9 gene. These findings provide a basis for future research on mutations in BMP15, GDF9, and BMPR1B genes in goats.
Article
Obstetrics & Gynecology
Isao Tamura, Mai Kawamoto-Jozaki, Taishi Fujimura, Yumiko Doi-Tanaka, Haruka Takagi, Yuichiro Shirafuta, Yumiko Mihara, Toshiaki Taketani, Hiroshi Tamura, Norihiro Sugino
Summary: In Japanese women, fertilization rates are significantly lower in oocytes from small follicles compared to those from large follicles, but if fertilized successfully, the developmental potential of oocytes from small follicles is comparable to those from large follicles. In ICSI, the developmental potential of mature oocytes is not related to follicular size.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2021)
Article
Environmental Sciences
Si-Cheng Zhao, Zhi-Ran Xu, Chang-Long Xu, Quan-Kuo He, Guang-Ming Yang, Yan-Ping Li, Yu-Shen Luo, Hai-Long Wang, Zhong-Quan Qi, Yu Liu
Summary: Exposure to nickel sulfate negatively affects ovarian homeostasis and oocyte quality, triggering ovarian fibrosis and inflammation, and causing structural and functional disorders of mitochondria.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biology
Irene Menendez-Blanco, Sandra Soto-Heras, Maria G. Catala, Anna-Rita Piras, Dolors Izquierdo, Maria-Teresa Paramio
Article
Reproductive Biology
Anna Rita Piras, Federica Ariu, Laura Falchi, Maria Teresa Zedda, Salvatore Pau, Eleonora Schianchi, MariaTeresa Paramio, Luisa Bogliolo
Article
Reproductive Biology
Irene Menendez-Blanco, Sandra Soto-Heras, Maria G. Catala, Montserrat Roura, Dolors Izquierdo, Maria-Teresa Paramio
Article
Agriculture, Dairy & Animal Science
I. Contreras-Solis, M. Catala, S. Soto-Heras, M. Roura, M. T. Paramio, D. Izquierdo
Summary: The study showed that oocytes from follicles >= 3 mm had higher concentrations of E-2 and P-4, ATP levels, and blastocyst development rate compared to those from <3 mm follicles. The results suggest that the E-2:P-4 ratio in follicles could be used as a reference for designing IVM medium and improving in vitro embryo production in lambs.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Tania Garcia-Martinez, Iris Martinez-Rodero, Joan Roncero-Carol, Meritxell Vendrell-Flotats, Jaume Gardela, Alfonso Gutierrez-Adan, Priscila Ramos-Ibeas, Adam Z. Higgins, Teresa Mogas
Summary: This study aimed to investigate the permeability of bovine oocyte plasma membrane to water and cryoprotectants, with a focus on the role of aquaporin 7 (AQP7). The results showed that cryoprotectants stimulated the expression of AQP3 and AQP7, with dimethyl sulfoxide upregulating AQP3 expression and ethylene glycol upregulating AQP7 expression. Additionally, overexpression of AQP7 increased the permeability of oocytes to water. These findings provide valuable insights for improving the cryopreservation protocols of bovine oocytes.
Article
Biochemistry & Molecular Biology
Dolors Izquierdo, Montserrat Roura, Miriam Perez-Trujillo, Sandra Soto-Heras, Maria-Teresa Paramio
Summary: The size of follicles in goats affects the competence of embryo oocytes, regardless of the age of the females. This study analyzed the composition of fatty acids and metabolites in follicular fluid recovered from small and large follicles of prepubertal goats and follicles of adult goats. Significant differences were found in the composition of fatty acids and metabolites between adult and prepubertal follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Erika Alina Ordonez-Leon, Iris Martinez-Rodero, Tania Garcia-Martinez, Manel Lopez-Bejar, Marc Yeste, Elena Mercade, Teresa Mogas
Summary: This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. The results suggest that adding 100 μg/mL EPS ID1 to the vitrification media can improve the re-expansion and hatching ability of embryos and modulate apoptosis positively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Iris Martinez-Rodero, Albert Salas-Huetos, Alina Ordonez-Leon, Carlos Olegario Hidalgo, Marc Yeste, Elena Mercade, Teresa Mogas
Summary: This study aimed to optimize the vitrification/in-straw warming protocol of in vitro-produced blastocysts by adding EPS ID1 to the vitrification media. The results showed that the addition of EPS ID1 not only promoted blastocyst survival and hatching rates, but also modified the expression of genes associated with better embryo quality.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Reproductive Biology
Nuria Gonzalez-Rodriguez, Iris Martinez-Rodero, Jakob Scherzer, Simone Jung, Myriam Reichenbach, Yury Zablotski, Christiane Otzdorff, Holm Zerbe, Teresa Mogas
Summary: In the cattle industry, using genotyping and cryopreservation technologies combined with in vivo or in vitro embryo production allows the selection and preservation of embryos with desirable traits. This study assessed the efficiency of a vitrification method for in-straw warming of biopsied bovine embryos. Results showed that both vitrification methods produced similar outcomes for intact and biopsied embryos, and vitrification did not affect the viability of biopsied embryos to develop into pregnancies.
Article
Biochemistry & Molecular Biology
Na-Young Rho, Teresa Mogas, W. Allan King, Laura A. Favetta
Summary: This study investigates the role of the TSPY gene in early male embryo development. The copy number, mRNA, and protein levels of TSPY were analyzed in embryos at different developmental stages. The results suggest that the TSPY gene plays a crucial role in male embryo development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Joanna M. G. Souza-Fabjan, Ribrio I. T. P. Batista, Lucas F. L. Correia, Maria Teresa Paramio, Jeferson F. Fonseca, Vicente J. F. Freitas, Pascal Mermillod
Summary: The market for small ruminant invitro-produced embryos is currently almost non-existent, possibly due to the lower added value of small ruminants, lower commercial demand, and fewer qualified professionals interested in the area. Additionally, there are fewer research groups working on small ruminant IVEP compared to those working with cattle and pigs. Despite the complexity of logistics in oocyte collection from live small ruminant females compared to cows, the overall outcomes of IVEP, in terms of blastocyst production, are similar.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.