Journal
THERIOGENOLOGY
Volume 73, Issue 7, Pages 893-899Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2009.11.013
Keywords
Follicular fluid; In vitro fertilization; In vitro maturation; Porcine oocyte; Rotating culture
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The objective was to investigate the effects of oxygen tension and follicle cells (FCs) during in vitro maturation of porcine oocytes in only porcine (Sus scrofa domesticus) follicular fluid (pFF), using static and non-static (rotating) culture systems, on the nuclear maturation and subsequent in vitro fertilization of the oocytes. In the first experiment, cumulus-oocyte complexes (COCs) were matured for 48 h in pFF supplemented with (+) or without (-) FCs (5.2 x 10(6) cells/mL), using the static (S) and rotating (R) culture systems (+FC/S, FC/S, +FC/R, and FC/R) under 5% or 20% O-2. Co-culture with FCs in the static culture system (+FC/S) had a detrimental effect on the meiotic competence of oocytes, whereas co-culture with FCs in the rotating culture system (+FC/R) increased maturation rates. In both culture systems, oxygen tension had no apparent effects on meiotic competence of oocytes, irrespective of culture system and FC addition. In the second experiment, COCs were matured under 5% or 20% O-2 using the FC/S or +FC/R culture systems and then fertilized. Oxygen tension had no significant effects on fertilization parameters, irrespective of the culture system. The rotating culture system increased rates of sperm penetration and male pronuclear formation and decreased polyspermic fertilization compared with the static culture system (P < 0.05). In conclusion, both FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the FC/S culture system for promoting fertilization. (C) 2010 Elsevier Inc. All rights reserved.
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