Article
Biochemical Research Methods
Yaqi Zhao, Akihiro Isozaki, Maik Herbig, Mika Hayashi, Kotaro Hiramatsu, Sota Yamazaki, Naoko Kondo, Shinsuke Ohnuki, Yoshikazu Ohya, Nao Nitta, Keisuke Goda
Summary: Intelligent image-activated cell sorting (iIACS) utilizes artificial intelligence (AI) algorithms for high-throughput image-based sorting of cells, solving the issue of flow speed fluctuation by predicting the arrival time of cells at the sort point and activating sorting accordingly. The accuracy of sort-timing prediction is improved through machine learning techniques.
Article
Cell Biology
Amandine Bonifay, Stephane Robert, Belinda Champagne, Paul-Remi Petit, Aude Eugene, Corinne Chareyre, Anne-Claire Duchez, Melanie Velier, Shirley Fritz, Loris Vallier, Romaric Lacroix, Francoise Dignat-George
Summary: This study establishes a method for detecting the surface antigenic characteristics of NDEVs by FCM and improves the sensitivity and accuracy of detection through the development of strategies. This study provides the possibility of measuring NDEVs as a potential biomarker in clinical practice more valuable.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Engineering, Environmental
Jonghun Lim, Yuchan Ahn, Hyungtae Cho, Junghwan Kim
Summary: This study proposes an optimal strategy for sorting plastic waste to improve recycling efficiency and reduce costs. Using a developed model, the optimal sorting strategy is identified and sensitivity analysis is conducted. The results show that the optimal strategy increases sorting efficiency by 4% and significantly reduces overall costs.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2022)
Article
Engineering, Environmental
Andr C. Pereira, Ana Tenreiro, Rogerio Tenreiro, Monica V. Cunha
Summary: A new innovative single-cell workflow combining multiple techniques was developed to detect, quantify, and sort M. bovis cells in environmental matrices. The method showed high efficiency and accuracy in assessing cell viability and molecular characteristics of M. bovis, providing new insights into tuberculosis research.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Veterinary Sciences
Patricia Pinto-Pinho, Ana F. Ferreira, Rosario Pinto-Leite, Margarida Fardilha, Bruno Colaco, Inmaculada Parrilla Riera, Athina Basioura, Junwei Li
Summary: This review article provides an overview of current and future developments in rabbit sperm sexing technologies, as well as the implications and challenges of implementing these methods in the cuniculture industry.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Ferran Garriga, Marc Llavanera, Estel Vinolas-Verges, Sandra Recuero, Carolina Tamargo, Ariadna Delgado-Bermudez, Marc Yeste
Summary: The presence of GSTM3 in bovine sperm was confirmed in this study, and high levels of GSTM3 may be an in vivo subfertility biomarker in cattle sperm, indicative of defective spermatogenesis and/or epididymal maturation.
Article
Microbiology
Samuel Bellais, Melanie Nehlich, Maryne Ania, Aurore Duquenoy, Wilfrid Mazier, Ger van den Engh, Jan Baijer, Nicole Simone Treichel, Thomas Clavel, Ilia Belotserkovsky, Vincent Thomas
Summary: Flow cytometry and cell sorting under anaerobic conditions can be used to detect, separate, and cultivate gut commensal bacteria. This study successfully isolated the extremely oxygen sensitive species Faecalibacterium prausnitzii and the under-represented keystone species Christensenella minuta, demonstrating the specificity of the antibodies and the effectiveness of the sorting strategies.
Article
Biochemical Research Methods
Sabrina Gacem, Maria Castello-Ruiz, Carlos O. Hidalgo, Carolina Tamargo, Pilar Santolaria, Carles Soler, Jesus L. Yaniz, Miguel A. Silvestre
Summary: The prediction of male or semen fertility potential remains a challenge. This study analyzed the in vitro parameters and proteome of spermatozoa in high- and low-fertility bulls. The results showed that high-fertility bulls had higher sperm motility and differential protein abundance related to energy production and motility, while low-fertility bulls had differential protein abundance related to protein processing and folding.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Agronomy
Shan Yang, Petr Capal, Jaroslav Dolezel, Xueting Li, Wang Qian, Zhiqiang Wang, Kai Zeng, Peiting Li, Hongkai Zhou, Rui Xia, Muqing Zhang, Zuhu Deng
Summary: This study reported on the flow cytometric sorting and sequencing of a single chromosome of a wild relative of sugarcane. The analysis provided insights into the genome composition and phylogenetic relationship of the chromosome. These findings will support molecular improvement and genetic breeding of sugarcane.
Article
Agriculture, Dairy & Animal Science
Anna Dziekonska, Marek Lecewicz, Agnieszka Partyka, Wojciech Nizanski
Summary: Flow cytometry (FC) is a recommended technique for assessing sperm quality, while fluorescence microscopy (FM) is often used as an alternative when FC is not accessible. In this study, FC and FM were used to evaluate the functionality of various organelles in European red deer epididymal spermatozoa stored in liquid nitrogen. The results showed that refrigerated storage of the epididymides for 12 hours had no significant effect on sperm quality before cryopreservation, but significantly influenced the percentage of early necrotic sperm after thawing. Despite differences in results, both FM and FC revealed significant correlations between examined variables, except for mitochondrial activity. The study concluded that European red deer spermatozoa stored in the epididymides at 2-4 degrees C for 12 hours can be successfully cryopreserved, with FM being better suited for evaluating mitochondrial activity and FC being more useful for assessing DNA fragmentation.
Article
Reproductive Biology
Camile Sanches Silva, Eliane Vianna da Costa-e-Silva, Margot Alves Nunes Dode, Andrielle Thainar Mendes Cunha, Wagner Rodrigues Garcia, Breno Fernandes Barreto Sampaio, Juliana Correa Borges Silva, Francisco Eduardo Machado Vaz, Karl Kerns, Peter Sutovsky, Eriklis Nogueira
Summary: Despite low usage in Brazilian bovine herds, artificial insemination is the most important form of biotechnology in animal reproduction. This study developed a novel composite model using qualitative attributes of semen to improve the prediction of bull fertility.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Environmental Sciences
Juliana Moreira Mendonca Gomes, Ives Charlie-Silva, Anderson Kenedy Santos, Rodrigo Ribeiro Resende, Juliana A. S. Gomes, Andrea Teixeira de Carvalho, Jose Dias Correa Junior
Summary: This study proposes flow cytometry as a method to detect changes in blood cell populations in living fish without euthanizing animals or using cell markers. By combining cell sorting and light microscopy techniques, populations of erythrocytes and lymphocytes were detected, revealing systemic deleterious effects in fish blood analysis.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Di Tian, Caiyan Wang, Yunfei Liu, Yueyue Zhang, Adriano Caliari, Hui Lu, Yang Xia, Boying Xu, Jian Xu, Tetsuya Yomo
Summary: Cell morphology is an important trait for tracking adaptation and evolution. Advances in quantitative analytical techniques based on optical properties allow for easy determination and tracking of morphology. Using fluorescence-activated cell sorting (FACS) in experimental evolution, stable mutants with distinct morphologies can be obtained.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Behavioral Sciences
Baptiste Martinet, Kimberly Przybyla, Camille Michielsens, Serge Aron
Summary: The transition from diploid-female egg production to haploid-male egg production during the decline phase of bumblebee colonies is associated with a decrease in sperm quantity and quality stored in the queen spermatheca over time. This study provides important insights into the reproductive mechanism of social Hymenoptera.
BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY
(2023)
Article
Chemistry, Analytical
John C. Sharpe, John S. Mitchell, Ling Lin, Hernanya Sedoglavich, Richard J. Blaikie
ANALYTICAL CHEMISTRY
(2008)
Article
Chemistry, Analytical
Donald Wlodkowic, Khashayar Khoshmanesh, John C. Sharpe, Zbigniew Darzynkiewicz, Jonathan M. Cooper
ANALYTICAL CHEMISTRY
(2011)
Article
Physics, Applied
N. Sedoglavich, R. Kuennemeyer, J. C. Sharpe
APPLIED PHYSICS LETTERS
(2009)
Article
Materials Science, Multidisciplinary
N. Sedoglavich, R. Kuennemeyer, S. R. Talele, J. C. Sharpe
CURRENT APPLIED PHYSICS
(2008)
Article
Cell & Tissue Engineering
Ruud Hulspas, Lydia Villa-Komaroff, Erin Koksal, Kenol Etienne, Patricia Rogers, Matt Tuttle, Olle Korsgren, John C. Sharpe, David Berglund
Article
Optics
Nemanya Sedoglavich, John C. Sharpe, Rainer Kunnemeyer, Sergey Rubanov
Article
Biochemical Research Methods
K. Mutafopulos, P. Spink, C. D. Lofstrom, P. J. Lu, H. Lu, J. C. Sharpe, T. Franke, D. A. Weitz
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.