Article
Pharmacology & Pharmacy
Shehreen Amjad, Md Saidur Rahman, Won-Ki Pang, Do-Yeal Ryu, Elikanah Olusayo Adegoke, Yoo-Jin Park, Myung-Geol Pang
Summary: In this study, exposure to a phthalate mixture resulted in decreased sperm motility and hyperactivity, compromised sperm ability to generate ATP, induced abnormal capacitation and acrosome reaction, upregulated protein tyrosine phosphorylation via a protein kinase-A-dependent pathway, and significantly affected fertilization and early embryonic development. The findings suggest that the phthalate mixtures adversely affected sperm processes and ultimately led to poor fertilization rates and repressed embryonic development, with the lowest observed adverse effect dose being <1 µg/mL in vitro.
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Gabriela Carrasquel Martinez, Andres Aldana, Jorge Carneiro, Claudia Lydia Trevino, Alberto Darszon
Summary: Mammalian sperm capacitation, involving biochemical and physiological modifications, is a crucial step for successful fertilization. This study showed that the acrosome pH gradually increases during human sperm capacitation, and the V-ATPase plays a key role in regulating the acrosome acidity. These findings provide important insights into the factors influencing sperm fusion and fertilization.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Cell Biology
Mina Pencheva, Donka Keskinova, Pavel Rashev, Yvetta Koeva, Nina Atanassova
Summary: Testicular angiotensin converting enzyme (ACE) plays a crucial role in male reproduction and fertility. Research shows that the proper distribution of tACE in the neck and mid-piece of sperm is essential for normal sperm motility. Different patterns of tACE expression were observed in pathological semen samples compared to normospermia.
Article
Reproductive Biology
Z. Bowker, S. Goldstein, H. Breitbart
Summary: Sperm cells must undergo the acrosome reaction in order to penetrate the egg. Capacitation, a series of biochemical changes, is necessary for this process to occur. This study discusses the role of protein acetylation in protecting bovine sperm from premature acrosome reaction. The results suggest a PKA-independent and EPAC-CaMKII dependent mechanism involving hyperacetylation to prevent acrosome reaction.
Article
Environmental Sciences
Anandha Rao Ravula, Suresh Yenugu
Summary: Studies on unintentional intake of pyrethroid pesticides similar to actual human exposure settings are rare. Exposure to low and high doses of pyrethroid mixture for 15 months resulted in abnormal lipid profile, liver function, and antioxidant enzyme activities, as well as damage to anatomical architecture in various tissues. This exposure also led to decreased sperm count, enzyme activities, impaired capacitation and acrosome reaction, and perturbations in gene expression related to male gamete production.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemical Research Methods
Renzheng Zhang, Xian Guo, Chunnian Liang, Jie Pei, Pengjia Bao, Mancai Yin, Fude Wu, Min Chu, Ping Yan
Summary: This study used a quantitative proteomic profiling approach to analyze the protein level changes in frozen-thawed Ashidan yak spermatozoa during fertilization. The results revealed differential protein abundances during sperm capacitation and acrosome reaction induction, with enrichment in different signaling pathways. Superoxide dismutase 1 and heat shock protein 90 alpha were identified as regulators of sperm capacitation and acrosome reaction.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wang, Hongjie Qiao, Pengxiang Wang, Yuan Wang, Danian Qin
Summary: Zdhhc19 knockout mice did not show disruption in spermatogenesis but exhibited male infertility with defects in sperm morphology and functions, highlighting the essential role of Zdhhc19 in sperm production and fertility in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Lijun Shan, Yuhao Chai, Tian Gao, Kun Li, Jianmin Yu, Fei Liang, Ya Ni, Peibei Sun
Summary: PFOS and PFOA, persistent organic pollutants, have harmful effects on human health, especially on male reproduction. They inhibit sperm capacitation and fertilization by affecting hormone levels, spermatogenesis, and sperm quality. The exact mechanisms are still unclear.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Developmental Biology
Melanie Balbach, Lubna Ghanem, Thomas Rossetti, Navpreet Kaur, Carla Ritagliati, Jacob Ferreira, Dario Krapf, Lis C. Puga Molina, Celia Maria Santi, Jan Niklas Hansen, Dagmar Wachten, Makoto Fushimi, Peter T. Meinke, Jochen Buck, Lonny R. Levin
Summary: sAC, essential for male fertility, is required for sperm capacitation and motility. A new sAC inhibitor, TDI-10229, disrupts capacitation-induced changes in mouse and human sperm, showing potential as non-hormonal contraceptives for women.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biology
Jesus L. Yaniz, Inmaculada Palacin, Miguel A. Silvestre, Carlos Olegario Hidalgo, Carolina Tamargo, Pilar Santolaria
Summary: The study found that assessing sperm quality with the ISAS3Fun method can more accurately distinguish between high- and low-fertility bulls, compared to conventional in vitro sperm quality tests.
Review
Biochemistry & Molecular Biology
Cameron C. Gardner, Paul F. James
Summary: Na+/H+ exchangers (NHEs) are important regulators of pH in eukaryotic cells and play crucial roles in sperm function and male fertility. Mammalian sperm express multiple NHE isoforms that contribute to various aspects of sperm physiology and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Steven Serafini, Cristian O'Flaherty
Summary: Spermatozoa undergo capacitation, a process involving biochemical and morphological changes to enable fertilization. Reactive oxygen species (ROS) play a dual role in capacitation, regulating cellular processes at physiological levels but causing damage at high concentrations. Understanding redox signaling and phosphorylation events in sperm is crucial for fertility and developing treatments for male infertility.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Physiology
Renzheng Zhang, Chunnian Liang, Xian Guo, Pengjia Bao, Jie Pei, Fude Wu, Mancai Yin, Min Chu, Ping Yan
Summary: This study analyzed the phosphorylation changes in frozen-thawed yak sperm during capacitation and acrosome reaction using phosphoproteomics. It identified proteins involved in sperm regulation, motility, energy metabolism, and flagella function, and revealed the role of phosphorylated proteins in the cAMP/PKA signaling pathway. The protein-protein interaction analysis showed the significance of differentially phosphorylated proteins in various aspects of sperm function.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Byeong-Mu Lee, Yoo-Jin Park, Won-Ki Pang, Do-Yeal Ryu, Md Saidur Rahman, Dae-Young Lee, Myung-Geol Pang
Summary: The activation of mitochondria in spermatozoa during capacitation leads to the changes in biological and morphological features of spermatozoa that are necessary for fertilization. This study focused on identifying the changes in mitochondrial proteins during sperm capacitation and their regulation of male fertility using boar spermatozoa. The expression of different mitochondrial proteins varied during sperm capacitation, depending on the fertility status of the boars.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.