Editorial Material
Biochemistry & Molecular Biology
Hau C. Kwaan
Summary: The fibrinolytic system is composed of plasmin, plasminogen, activators, and inhibitors. It plays essential roles in physiological processes and the pathogenesis of disorders. Recent advancements offer opportunities for drug development in treating these disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Alamelu G. Bharadwaj, Ryan W. Holloway, Victoria A. Miller, David M. Waisman
Summary: This review discusses in detail how tumor cells use the plasminogen-activation system to attack surrounding tissues, focusing on the role of the plasminogen receptor p11. It highlights the importance of the tumor microenvironment in cancer progression and emphasizes the key role of the plasminogen activation system components in modulating the TME.
Article
Agriculture, Dairy & Animal Science
James D. Gillis, Linda M. Penfold, Natalie D. Mylniczenko
Summary: This study explored the reproductive biology and sperm cryopreservation of southern stingrays, finding correlations between plasma testosterone levels and semen quality, as well as the potential benefits of a slow freezing method in improving post-thaw sperm survival.
Article
Agriculture, Dairy & Animal Science
Sang-Hee Lee, Seunghyung Lee
Summary: This study aimed to investigate the effects of melatonin and silymarin on the motility and viability of frozen-thawed boar semen, as well as the production of reactive oxygen species (ROS) and nitric oxide (NO). The results showed that melatonin and silymarin reduced ROS and NO production in frozen-thawed sperm and enhanced sperm viability. Therefore, melatonin and silymarin play an important role as antioxidants in semen cryopreservation.
Article
Reproductive Biology
Jagger Segura Ochoa, Romina Nunez Favre, Maria Florencia Garcia, Maria Cecilia Stornelli, Washington Carrasco Sangache, Ramiro Rearte, Luzbel de la Sota, Maria Alejandra Stornelli
Summary: This study aimed to assess the effect of a commercially-available GnRH vaccine for swine on suppressing sperm production in male cats. The results showed that the vaccine effectively reduced sperm quality, testicular volume, and serum testosterone concentration in treated cats. These findings are consistent with previous studies and suggest that the vaccine has potential for reproductive control in cats.
Review
Biochemistry & Molecular Biology
Adam Miszta, Dana Huskens, Demy Donkervoort, Molly J. M. Roberts, Alisa S. Wolberg, Bas de Laat
Summary: Fibrinolysis is a crucial process in wound healing, with plasmin playing a central role in dissolving clots. Recent advancements have enabled the quantification of plasmin generation (PG) and inhibition kinetics during fibrinolysis. Understanding plasmin kinetics aids in identifying fibrinolytic dysfunction and investigating the link between abnormal fibrin dissolution and disease pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Olga Mitjana, Raquel Ausejo, Noelia Mendoza, Joaquin Miguel, Maria Teresa Tejedor, Ana Maria Garrido, Maria Victoria Falceto
Summary: The study found that melatonin had no significant impact on sperm motility, a negative effect on acrosomal defects, apoptosis, and viability in the decreasing photoperiod, while it had beneficial effects on acrosomal defects and apoptosis in the increasing photoperiod, and increased viability in the short term.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Obstetrics & Gynecology
Lidia Minguez-Alarcon, Paige L. Williams, Irene Souter, Jennifer B. Ford, Ramy Abou Ghayda, Russ Hauser, Jorge E. Chavarro
Summary: Men working non-daytime/rotating shifts and those with physically demanding jobs have higher sperm concentration and total sperm count as well as higher estradiol and total testosterone concentrations.
HUMAN REPRODUCTION
(2023)
Article
Fisheries
Sen Yang, Zhilu Zeng, Xinghan Chen, Bin Fan, Zining Meng
Summary: This study aimed to assess the effect of melatonin addition on post-thaw sperm function of giant grouper. The results showed that melatonin improved the total motility, progressive motility, mitochondrial membrane potential, and velocity of frozen-thawed sperm. Melatonin also reduced apoptotic-like changes, increased sperm viability, and decreased DNA damage. Additionally, melatonin supplementation improved the fertility and hatching rate of cryopreserved giant grouper sperm, with a recommended concentration of 1-2 μM in the cryomedium.
AQUACULTURE REPORTS
(2023)
Article
Engineering, Environmental
Tingting Zhu, Bin Liu
Summary: In this study, peracetic acid (PAA), ultraviolet (UV)/PAA, and ultrasonic (US)/PAA pre-oxidation were used to improve the ultrafiltration (UF) performance for algae-laden water treatment. The results showed that PAA at a dosage of 10 mg/L achieved the best performance with a 76.26% reduction in membrane fouling resistance. Low dosage of UV/PAA effectively controlled fouling by enhancing the degradation of dissolved organics. The study also revealed that moderate oxidation, with high organic degradation and low cell rupture, was the key principle for algae-laden water treatment.
Article
Veterinary Sciences
Yan Lin, De Wu, Lianqiang Che, Zhengfeng Fang, Shengyu Xu, Bin Feng, Yong Zhuo, Jian Li, Caimei Wu, Junjie Zhang, Lujie Li
Summary: This study investigated the effects of fibre supplementation on testis development in growing boars and found that it increased semen production without affecting growth performance or testicular volume. Fibre supplementation resulted in lower serum cholesterol and LDL levels, but higher testicular cholesterol, triglyceride, and LDL levels. It also increased Leydig cell density and up-regulated the expression of genes related to hormone synthesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
A. S. El-Shalofy, A. M. Shahat, M. G. Hedia
Summary: This study investigated the influence of melatonin injection on testicular hemodynamics, echotexture, plasma testosterone concentration, and sperm quality characteristics in Ossimi rams during the non-breeding season. The results showed that melatonin significantly improved testicular vascular irrigation, testosterone production, and sperm quality parameters, including motility, viability, and normal morphology. These findings suggest that melatonin administration can enhance testicular function in Ossimi rams.
Article
Reproductive Biology
Minghui Yang, Shengyu Guan, Jingli Tao, Kuanfeng Zhu, Dongying Lv, Jing Wang, Guangdong Li, Yuefeng Gao, Hao Wu, Jinghao Liu, Lin Cao, Yao Fu, Pengyun Ji, Zhengxing Lian, Lu Zhang, Guoshi Liu
Summary: The study found that endogenous melatonin can regulate male reproductive function by inhibiting Leydig cell apoptosis, increasing testosterone production, and improving sperm quality. Melatonin exerts these effects by targeting the mitochondrial apoptotic pathway and upregulating testosterone synthesis-related genes in Leydig cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Alexandra Alevra, Athanasios Exadactylos, Eleni Mente, Serafeim Papadopoulos
Summary: Cryopreservation of fish sperm is a rapidly evolving technique with potential benefits in genetic reproduction programs and conservation efforts. The use of cryoprotectants and antioxidants, such as melotonin, is crucial to minimize damage to the sperm during the freezing and warming processes. Further research is needed to explore the positive effects of melatonin as a sperm cryoprotectant in fish species and establish improvement protocols for cryopreservation.
Article
Agriculture, Dairy & Animal Science
Osman Ahmed, Khoboso Lehloenya, Masindi Mphaphathi, Abubeker Hassen
Summary: The study evaluated the supplementation effects of Acacia mearnsii tannin extract (TE) and encapsulated tannin extract (ETE) on the reproductive performance and oxidative status of South African Mutton Merino rams. It was found that encapsulation of TE provided the maximum benefit on sperm quality, and the seasonal effect had a significant impact on testicular measurements.
Article
Nutrition & Dietetics
Ioannis A. Kourtzidis, Andreas T. Stoupas, Ioannis S. Gioris, Aristidis S. Veskoukis, Nikos V. Margaritelis, Maria Tsantarliotou, Ioannis Taitzoglou, Ioannis S. Vrabas, Vassilis Paschalis, Antonios Kyparos, Michalis G. Nikolaidis
JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION
(2016)
Article
Physiology
I. A. Kourtzidis, C. F. Dolopikou, A. N. Tsiftsis, N. V. Margaritelis, A. A. Theodorou, I. A. Zervos, M. P. Tsantarliotou, A. S. Veskoukis, I. S. Vrabas, V. Paschalis, A. Kyparos, M. G. Nikolaidis
EXPERIMENTAL PHYSIOLOGY
(2018)
Article
Food Science & Technology
M. P. Tsantarliotou, S. N. Lavrentiadou, D. A. Psalla, I. E. Margaritis, M. G. Kritsepi, I. A. Zervos, M. I. Latsari, V. G. Sapanidou, I. A. Taitzoglou, Z. M. Sinakos
FOOD AND CHEMICAL TOXICOLOGY
(2019)
Article
Food Science & Technology
Alexandros Priftis, Vasiliki Soursou, Anthi-Styliani Makiou, Fotis Tekos, Aristidis S. Veskoukis, Maria P. Tsantarliotou, Ioannis A. Taitzoglou, Dimitrios Kouretas
FOOD AND CHEMICAL TOXICOLOGY
(2019)
Article
Biology
Ioannis Margaritis, Katerina Angelopoulou, Sophia Lavrentiadou, Ilias C. Mavrovouniotis, Maria Tsantarliotou, Ioannis Taitzoglou, Alexandros Theodoridis, Aristidis Veskoukis, Efthalia Kerasioti, Dimitrios Kouretas, Ioannis Zervos
JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI
(2020)
Article
Pharmacology & Pharmacy
Christina Karavasili, Ioannis I. Andreadis, Maria P. Tsantarliotou, Ioannis A. Taitzoglou, Paschalina Chatzopoulou, Dimitrios Katsantonis, Constantinos K. Zacharis, Catherine Markopoulou, Dimitrios G. Fatouros
Article
Oncology
Elena E. Tzekaki, George Geromichalos, Sophia N. Lavrentiadou, Maria P. Tsantarliotou, Anastasia A. Pantazaki, Angelos Papaspyropoulos
Summary: Elevated levels of PAI-1 are inversely correlated with ER and PR expressions in estrogen/progesterone-responsive human malignancies. In silico molecular docking analysis identified oleuropein as a potent PAI-1-binding molecule with increased affinity compared to other olive oil constituents. Oleuropein acts as a natural PAI-1 inhibitor by destabilizing PAI-1 levels selectively in ER-/PR- breast cancer cells, leading to downstream caspase activation and cell growth inhibition.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Ilias Michos, Maria Tsantarliotou, Constantin M. Boscos, Georgios Tsousis, Athina Basioura, Eleni D. Tzika, Panagiotis D. Tassis, Aristotelis G. Lymberopoulos, Ioannis A. Tsakmakidis
Summary: The study evaluated the relationship between boar sperm characteristics and sperm/seminal plasma proteins with main parameters of field fertility in swine. It found that immotile sperm and sperm with biochemically active plasma membranes influenced the number of live-born piglets and litter size of >= 12 piglets. Specific proteins like osteopontin 70 and glutathione peroxidase 5 were also found to affect farrowing rate and fertility when combined with certain sperm characteristics. The evaluation of semen quality variables combined with specific sperm or seminal plasma proteins could provide useful information on in vivo fertilizing capacity of semen doses.
Article
Agriculture, Dairy & Animal Science
Vasiliki Sapanidou, Sophia N. Lavrentiadou, Michela Errico, Ioannis Panagiotidis, Dimitrios Fletouris, Ioannis Efraimidis, Ioannis Zervos, Ioannis Taitzoglou, Bianca Gasparrini, Maria Tsantarliotou
Summary: The study evaluated the potential of crocin in bovine sperm cryopreservation and found that it improved sperm quality during the freeze/thaw process, particularly in terms of sperm viability and motility parameters.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Review
Agriculture, Dairy & Animal Science
Vasiliki Sapanidou, Maria P. Tsantarliotou, Sophia N. Lavrentiadou
Summary: This review discusses the role of reactive oxygen species (ROS) and oxidative stress (OS) in bovine sperm physiology, highlighting their association with male infertility and low fertility rates in Assisted Reproductive Techniques (ART). Antioxidant supplementation is considered as a potential solution to overcome OS-related infertility and improve assisted reproduction outcomes. The article summarizes different studies that have investigated the potential sources of ROS in ART settings and the impact of antioxidant supplementation on semen quality and pregnancy outcome, particularly in farm animals. However, there is limited evidence to support the effectiveness of antioxidant supplementation on fertilization and embryo development in these animals.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Vasiliki Sapanidou, Maria Tsantarliotou, Sophia Lavrentiadou, Elena Tzekaki, Ioannis Efraimidis, Theodoros Lialiaris, Byron Asimakopoulos
Summary: The present study investigated the effect of pterostilbene, a natural phenolic compound, on bovine sperm quality. The results demonstrated that the addition of pterostilbene improved motility, viability, intracellular superoxide anion concentration, and acrosomal status of spermatozoa. This suggests that pterostilbene has potential benefits on sperm quality.
Article
Toxicology
Paraskevi Kouka, Fotios Tekos, Zoi Papoutsaki, Panagiotis Stathopoulos, Maria Halabalaki, Maria Tsantarliotou, Ioannis Zervos, Charitini Nepka, Jyrki Liesivuori, Valerii N. Rakitskii, Aristidis Tsatsakis, Aristidis S. Veskoukis, Demetrios Kouretas
TOXICOLOGY REPORTS
(2020)
Article
Veterinary Sciences
I. A. Michos, I. A. Tsakmakidis, M. Tsantarliotou, G. Tsousis, P. Hadweh, C. M. Boscos
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
(2019)
Article
Andrology
A. N. Kokoli, S. N. Lavrentiadou, I. A. Zervos, M. P. Tsantarliotou, M. P. Georgiadis, E. A. Nikolaidis, N. Botsoglou, C. M. Boscos, I. A. Taitzoglou
Article
Veterinary Sciences
I. A. Tsakmakidis, M. Tsantarliotou, I. A. Michos, A. G. Basioura, G. Tsousis, C. M. Boscos
POLISH JOURNAL OF VETERINARY SCIENCES
(2017)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.