Article
Obstetrics & Gynecology
Robert B. Hood, Donghai Liang, Youran Tan, Jennifer B. Ford, Irene Souter, Jorge E. Chavarro, Dean P. Jones, Russ Hauser, Audrey J. Gaskins
Summary: This study identified the metabolic pathways and metabolites in the serum and follicular fluid that are associated with peak estradiol levels and the number of mature oocytes. The serum metabolome mainly involves fatty acid and amino acid pathways, while the follicular fluid metabolome mainly involves lipid, vitamin, and hormone pathways. These findings provide insights into the biological mechanisms underlying follicular and oocyte development.
HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Di Zhang, Jia Lv, Rongxin Tang, Ying Feng, Yongfeng Zhao, Xiaoyang Fei, Richeng Chian, Qigui Xie
Summary: The study investigated exosomal microRNAs in human ovarian follicular fluid to explore their potential association with oocyte quality. Analysis revealed that several exosomal miRNAs were critical for maintaining oocyte quality, suggesting they may be potential biomarkers for evaluating oocyte quality, which is important in clinical settings.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Florentin-Daniel Staicu, Analuce Canha-Gouveia, Cristina Soriano-Ubeda, Juan Carlos Martinez-Soto, Evdochia Adoamnei, Jorge E. Chavarro, Carmen Matas
Summary: The levels of nitrite and nitrate in follicular fluid were found to be related to the yield of MII oocytes, and nitrate levels showed a suggestive inverse correlation with embryos with high potential of implantation. This suggests that nitrite and nitrate concentrations in follicular fluid may be useful in predicting ovarian response and embryo implantation potential.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anne-Sophie Neyroud, Regina Maria Chiechio, Gregory Moulin, Solene Ducarre, Claire Heichette, Aurelien Dupont, Mathieu Budzynski, Pascale Even-Hernandez, Maria Jose Lo Faro, Marina Yefimova, Valerie Marchi, Celia Ravel
Summary: The morphology and quantification of extracellular vesicles (EVs) in follicular fluid (FF) of healthy individuals were studied in this research. EVs play important roles in the development of female gametes, but limited knowledge exists regarding their morphology. The findings of this study provide a comprehensive morphological description and quantification of FF-derived EVs, serving as a reference for further research on EVs in ovarian diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medical Laboratory Technology
Hamid Reza Nejabati, Leila Roshangar, Mohammad Nouri
Summary: The decrease in reproductive potential with aging is attributed to the gradual decline in quantity and quality of ovarian reserve, which increases the risk of miscarriage, pregnancy loss, low ovarian stimulation, and oocyte abnormalities. Changes in cellular composition of follicles have been shown to contribute to the reduced fertility potential of older women. Moreover, extracellular vesicles (EVs), especially non-coding RNAs like miRNAs, have been found to play a role in cellular communication and their composition has been documented to alter with age, suggesting their potential as predictors of oocyte quality.
CLINICA CHIMICA ACTA
(2023)
Article
Endocrinology & Metabolism
Li Yu, Miao Liu, Shiji Xu, Zhenxin Wang, Te Liu, Jiaye Zhou, Doudou Zhang, Xi Dong, Baishen Pan, Beili Wang, Suying Liu, Wei Guo
Summary: This study evaluated the role of hormones and mitochondrial function in embryo development during in vitro fertilization cycles. The results showed that LH and FSH levels in follicular fluid could predict the formation of high-quality embryos, and embryo development was associated with mitochondrial function in the follicular fluid.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.
Article
Reproductive Biology
Bin Liu, Yongjie Liu, Shuman Li, Pingping Chen, Jun Zhang, Liping Feng
Summary: This study investigated the reproductive outcomes of BDNF treatment and potential mechanisms in aged mice. The results showed that BDNF treatment could improve ovarian function and fertility in aged mice by promoting ovarian cell proliferation and activating the TrkB and cyclin D1-creb signaling pathways. Therefore, targeting the BDNF-TrkB signaling pathway may be a potential novel therapeutic strategy to reverse ovarian aging.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Biology
Tomoko Kawai, JoAnne S. Richards, Masayuki Shimada
Summary: Research has shown that through modulating processes such as DNA methylation levels and histone modifications, equine chorionic gonadotropin can promote the proliferation and differentiation of granulosa cells, and the timing and sequence of these epigenetic events are crucial for the progression of granulosa cell differentiation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Chong Wang, Xiaoyang Fei, Hongyan Zhang, Wenjing Zhou, Zhaojun Cheng, Ying Feng
Summary: This study identified differentially expressed proteins in mature and immature oocytes using iTRAQ labelling and 2D LC-MS/MS. ACPP and CD5L were found to be differentially expressed between MII and Gv oocytes. Further measurements revealed significantly lower level of ACPP in the ovarian follicular fluid and cumulus cells of DOR patients, indicating poor oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Uzbekova, Ana-Paula Teixeira-Gomes, Aurelie Marestaing, Peggy Jarrier-Gaillard, Pascal Papillier, Ekaterina N. Shedova, Galina N. Singina, Rustem Uzbekov, Valerie Labas
Summary: In this study, 84 palmitoylated proteins were identified in bovine ovarian follicle cells, among which variations in abundance of these proteins during oocyte maturation were observed. The involvement of palmitoyl-proteins in oxidation-reduction processes, energy metabolism, protein localization, vesicle transport, stress response, and signaling pathways suggests that protein palmitoylation may play important roles in oocyte maturation and follicle communications mediated by ffEVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Berna Aslan Cetin, Pelin Ocal, Tulay Irez, Ezel Uslu, Kubra Irmak, Suat Karatas
Summary: The study found a correlation between sialic acid levels in follicular fluid and oocyte quality during in vitro fertilization (IVF), but not with clinical parameters. Sialic acid levels in the follicular fluid may play a role in normal oocyte maturation or act as a compensatory mechanism against reactive oxygen species during the maturation process.
REPRODUCTIVE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jianan Li, Zhourui Zhang, Yiqiu Wei, Pujia Zhu, Tailang Yin, Qiongqiong Wan
Summary: Ovarian reserve is crucial for female reproductive potential. Diminished ovarian reserve (DOR) leads to reduced number and quality of oocytes, and even with IVF-ET, the pregnancy and live birth rates are low. Metabolic analysis of follicular fluid revealed unique metabolic characteristics in DOR patients, providing insights into the pathogenesis and prediction of ovarian reserve function.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Euihyun Kim, Kihae Ra, Myung-Shin Lee, Geon A. Kim
Summary: Exosomes derived from porcine follicular fluid can improve the morphology and functionality of pig oocytes when added to the maturation medium. Therefore, it is suggested to use FF-derived exosomes in controlled experiments in embryology to enhance the reliability of experimental data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Eva Bongaerts, Katariina Mamia, Ilmatar Rooda, Richelle D. Bjorvang, Kiriaki Papaikonomou, Sebastian B. Gidlof, Jan I. Olofsson, Marcel Ameloot, Ernesto Alfaro-Moreno, Tim S. Nawrot, Pauliina Damdimopoulou
Summary: There is evidence indicating a link between exposure to ambient air pollution and decreased female fertility. This study found that black carbon particles from air pollution can reach the ovaries and follicular fluid, potentially impacting fertility.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.