Article
Plant Sciences
Hongwei Xu, Yingbo Li, Runhong Gao, Rugen Xu, Guimei Guo, Ruiju Lu, Nigel G. Halford, Zhiwei Chen, Chenghong Liu
Summary: The study improved the nitrogen use efficiency (NUE) of barley line DH45 through F-1 microspore embryogenesis and hydroponic field experiments, and investigated the molecular mechanisms behind its superior NUE compared to its parents using RNA-seq analysis. The results revealed enrichment of genes involved in nitrogen compound metabolism in DH45 under low nitrogen conditions.
Article
Plant Sciences
Anyong Zhang, Ting Zhao, Xue Hu, Yu Zhou, Yue An, Haiyi Pei, Dongfa Sun, Genlou Sun, Chengdao Li, Xifeng Ren
Summary: This study evaluated stem-related traits in barley and identified significant phenotypic correlations between lodging index (LI) and other stem traits. Several stable and major QTLs were detected, along with candidate genes related to plant development and stress response. The findings contribute to the understanding of barley stem morphology and provide a foundation for further genetic mapping.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haixiao Hu, Yujie Meng, Wenxin Liu, Shaojiang Chen, Daniel. E. E. Runcie
Summary: Recent advances in maize DH technology enable the efficient development of large numbers of DH lines. Phenotyping of haploid progenitors can accelerate the selection of DH lines. Multi-trait models improve genomic prediction accuracy for DH lines, but incorporating information from haploid lines does not further improve the prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Iara Goncalves dos Santos, Anderson Luiz Verzegnazzi, Jode Edwards, Ursula K. Frei, Nicholas Boerman, Leandro Tonello Zuffo, Luiz P. M. Pires, Gerald de la Fuente, Thomas Lubberstedt
Summary: This study demonstrates that spontaneous haploid genome doubling does not affect the performance of inbred lines and that BS39 genes have the potential to add novel alleles to temperate elite germplasm.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Nutrition & Dietetics
M. Christine Schleif, Shelby L. Havel, Michael D. Griswold
Summary: Retinoic acid, an active metabolite of vitamin A, plays important roles in the developmental processes of mammals, especially in spermatogenesis. It is crucial for the formation of the blood-testis barrier and spermatogonial differentiation, spermiation, and assisting in meiotic completion in male reproduction. However, research results on female oogenesis have shown varying outcomes.
Article
Plant Sciences
Surbhi Grewal, Veronica Guwela, Claire Newell, Cai-yun Yang, Stephen Ashling, Duncan Scholefield, Stella Hubbart-Edwards, Amanda Burridge, Alex Stride, Ian P. King, Julie King
Summary: This study generated 86 stable inherited doubled haploid (DH) wheat-T. urartu introgression lines, which were characterized using genotyping arrays and KASP markers to identify unique introgressions in a bread wheat background. The DH lines have accelerated the breeding process and enabled the early release of homozygous wheat-T. urartu introgression lines, which can greatly advance the identification of beneficial alleles for wheat improvement.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Rabouan Oubaidou, Samira Hentour, Chafika Houasli, Rachid Aboutayeb, Youness El Goumi, Ezzouhra El Maaiden, Fatima Gaboun, Ouafa Lamsaouri, Malika Fakiri
Summary: Salt stress is a major environmental factor affecting cereal productivity, and selecting salt tolerant genotypes is an effective way to overcome yield losses. This study compared phenotypic variability among doubled haploid barley lines and their parent under different salinity conditions. Results showed that traits like plant height and number of grains per spike play significant roles in determining grain yield, with number of fertile spikes per plant and number of grains per spike being indicative of salt tolerance in barley.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Muhammad Massub Tehseen, Fatma Aykut Tonk, Muzaffer Tosun, Harpinder Singh Randhawa, Ezgi Kurtulus, Izzet Ozseven, Behyan Akin, Ozge Nur Zulfuagaoglu, Kumarse Nazari
Summary: This study identified multiple QTLs controlling stripe rust resistance in wheat using an elite line with adult plant resistance. SNP markers flanking the QTL regions can aid in marker-assisted selection.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Nicole Pretini, Leonardo S. Vanzetti, Ignacio I. Terrile, Guillermo Donaire, Fernanda G. Gonzalez
Summary: In this study, QTL for grain weight (GW), grains per spike (GN), and related spike fertility traits were mapped using two DH populations. A total of 305 QTL were identified for 14 traits, with 12 QTL identified in multiple environments explaining over 10% of phenotypic variation in at least one environment. Eight hotspot regions were detected across chromosomes 1A, 2B, 3A, 5A, 5B, 7A, and 7B, highlighting the potential for marker assisted selection to improve spike yield potential.
Article
Plant Sciences
Alejandro Ledesma, Fernando Augusto Sales Ribeiro, Alison Uberti, Jode Edwards, Sarah Hearne, Ursula Frei, Thomas Lubberstedt
Summary: The molecular characterization analysis confirmed the loss of genetic variability from C0 to C17 through the recurrent selection process in maize breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Jahad Soorni, Zahra-Sadat Shobbar, Danial Kahrizi, Federica Zanetti, Kaveh Sadeghi, Sara Rostampour, Peter Gergo Kovacs, Attila Kiss, Iman Mirmazloum
Summary: This study investigated the diverse agronomic traits of 136 doubled haploid (DH) camelina lines, including seed yield, fatty acid composition, and protein content. The results showed significant diversity among the lines for these traits. The positive relationships observed suggest the potential for developing higher-yielding camelina cultivars with high seed oil content. The study also identified promising genotypes for further breeding to increase seed yield and improve food/nonfood traits.
Article
Agronomy
Muhammad Fuad Anshori, Bambang Sapta Purwoko, Iswari Saraswati Dewi, Sintho Wahyuning Ardie, Willy Bayuardi Suwarno
Summary: By utilizing a multivariate analysis approach, this study established the indirect selection index of doubled haploid rice to select lines adaptive to salinity stress. Through field and greenhouse experiments, a total of 28 DH rice lines were identified as adaptive to salinity stress based on good agronomic traits and salinity tolerance. The effectiveness of the indirect adaptability selection index was validated through field experiments in a saline area.
Article
Plant Sciences
Luiz M. Cabral, Hana P. Masuda, Helkin F. Ballesteros, Janice de Almeida-Engler, Marcio Alves-Ferreira, Karen L. G. De Toni, Fernanda M. Bizotto, Paulo C. G. Ferreira, Adriana S. Hemerly
Summary: This study investigates the role of the plant-exclusive protein ABAP1 in plant reproduction, showing that it regulates cell division and differentiation processes in both male and female gametophytes by binding to specific transcription factors and repressing their target genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Muniyandi Samuel Jeberson, Harinder Kumar Chaudhary, Rakesh Kumar Chahota, Shabir Hussain Wani
Summary: The study explores the potential of improving bread wheat using the rye genome, with wheat-rye substitutions and translocations being commonly utilized for resistance breeding. Crosses of different generations were used to assess various haploid induction parameters, and molecular cytogenetic analysis revealed an IBL.1RS translocation and substitution of the 5R chromosome in doubled haploids from BC2F3.
Article
Biotechnology & Applied Microbiology
Begona Echavarri, Luis Cistue
PLANT CELL TISSUE AND ORGAN CULTURE
(2016)
Article
Biotechnology & Applied Microbiology
Dustin Herb, Tanya Filichkin, Scott Fisk, Laura Helgerson, Patrick Hayes, Brigid Meints, Rebecca Jennings, Robert Monsour, Sean Tynan, Kristi Vinkemeier, Ignacio Romagosa, Matthew Moscou, Daniel Carey, Randy Thiel, Luis Cistue, Christopher Martens, William Thomas
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2017)
Article
Biotechnology & Applied Microbiology
Dustin Herb, Tanya Filichkin, Scott Fisk, Laura Helgerson, Patrick Hayes, Amanda Benson, Veronica Vega, Daniel Carey, Randy Thiel, Luis Cistue, Rebecca Jennings, Robert Monsour, Sean Tynan, Kristi Vinkemeier, Yueshu Li, Andrew Nguygen, Aaron Onio, Brigid Meints, Matthew Moscou, Ignacio Romagosa, William Thomas
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2017)
Article
Plant Sciences
Hiroshi Hisano, Brigid Meints, Matthew J. Moscou, Luis Cistue, Begoa Echavarri, Kazuhiro Sato, Patrick M. Hayes
PLANT CELL REPORTS
(2017)
Article
Plant Sciences
Xin Jin, Chao Bai, Ludovic Bassie, Carmina Nogareda, Ignacio Romagosa, Richard M. Twyman, Paul Christou, Changfu Zhu
Article
Plant Sciences
Lucia Perez, Erika Soto, Gemma Farre, Julia Juanos, Gemma Villorbina, Ludovic Bassie, Vicente Medina, Antonio Jesus Serrato, Mariam Sahrawy, Jose Antonio Rojas, Ignacio Romagosa, Pilar Munoz, Changfu Zhu, Paul Christou
PLANT CELL REPORTS
(2019)
Article
Agronomy
Davide Cammarano, Salvatore Ceccarelli, Stefania Grando, Ignacio Romagosa, Abdelkader Benbelkacem, Tanek Akar, Adnan Al-Yassin, Nicola Pecchioni, Enrico Francia, Domenico Ronga
EUROPEAN JOURNAL OF AGRONOMY
(2019)
Article
Food Science & Technology
Mariona Martinez-Subira, M. Paz Romero, Eva Puig, Alba Macia, Ignacio Romagosa, Marian Moralejo
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Food Science & Technology
Mariona Martinez-Subira, Maria-Paz Romero, Alba Macia, Eva Puig, Ignacio Romagosa, Marian Moralejo
Summary: The study found that different barley genotypes contain rich bioactive compounds and antioxidant capacity after sequential pearling, with husks having the highest antioxidant content. Even though current regulations require the elimination of inedible husks from human foods, they should be considered as valuable materials for animal feeds due to their high content of beneficial substances.
Article
Agriculture, Multidisciplinary
Mariona Martinez-Subira, Maria-Paz Romero, Marian Moralejo, Alba Macia, Eva Puig, Roxana Savin, Ignacio Romagosa
Summary: The study investigated the effect of post-anthesis thermal stress on barley bioactive compounds and antioxidant capacity, finding that thermal stress altered grain weight and size, as well as the relative composition of bioactive compounds. Heat stress had a direct impact on some phenolic compounds, leading to an increase in antioxidant capacity of barley grains.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Plant Sciences
Davide Cammarano, Domenico Ronga, Enrico Francia, Taner Akar, Adnan Al-Yassin, Abdelkader Benbelkacem, Stefania Grando, Ignacio Romagosa, Antonio Michele Stanca, Nicola Pecchioni
Summary: Heading time in barley is a key developmental stage controlling adaptation to the environment, influenced by agronomy and genetics. The study highlights the importance of optimal genotype/sowing date combinations in escaping extreme weather events and maximizing grain yield in the Mediterranean basin. Breeding activities should focus on allelic combinations with recessive VRN-H2 and EPS2 genes to minimize heat stress risks and maintain high yields.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Mariona Martinez-Subira, Marian Moralejo, Eva Puig, Maria-Paz Romero, Roxana Savin, Ignacio Romagosa
Summary: This study investigated the deposition patterns of various compounds in barley grains under different temperature conditions. Genotypic differences played a significant role in the duration of deposition, with antioxidant capacity decreasing over time. Heat stress had varying effects on the accumulation of phenolic compounds and antioxidant capacity, supporting the cultivation of food barley in high-temperature stress-prone areas.
Article
Biotechnology & Applied Microbiology
Neus Prieto-Diez, Tomas Ramo, Ignacio Romagosa, Maria-Paz Romero
Summary: This experiment validated and optimized a simple alternative micro-malting mesh-bag-based method that is more representative of industrial quality. The results showed that the malt quality obtained through the new alternative method was closer to industrial sample quality compared to the standard micro-malting laboratory equipment. There were no significant statistical allelopathic interactions between alternative bagged genotypes and genotypes used in the commercial batches.
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2023)
Article
Horticulture
Oreto Fayos, Begona Echavarri, M. Pilar Valles, Cristina Mallor, Ana Garces-Claver, Ana M. Castillo
Summary: This study aimed to establish a simple, inexpensive, and easily reproducible protocol for medium-term onion pollen storage. The results showed that dehydration was essential for pollen preservation at low relative humidity and temperatures below zero.
SCIENTIA HORTICULTURAE
(2022)
Article
Humanities, Multidisciplinary
Paloma Melgarejo, Ignacio Romagosa, Nuria Duran
ARBOR-CIENCIA PENSAMIENTO Y CULTURA
(2014)