Article
Biotechnology & Applied Microbiology
Dong Hyun Kim, So Young Park, Kyoung Heon Kim
Summary: In this study, various beta-galactosidases belonging to different glycoside hydrolase families were compared for their substrate specificities and kinetic parameters. Bga42A, originating from Bifidobacterium longum ssp. infantis ATCC 15,697, showed the highest substrate specificity and was chosen for the two-step process of high-titer L-AHG production from agarose.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
L. Joji Reddy, P. Suresh Kumar, Santhi Latha Pandrangi, Rajashekar Chikati, C. Srinivasulu, Akbar John, Arifullah Mohammed, Ramachandra Reddy Pamuru
Summary: The majority of the Earth's ecosystem is cold and frozen, providing a suitable environment for various microbes to thrive. Cold-adapted enzymes have significant biotechnological value, and the enzyme market is expected to grow due to increasing consumer preference for packaged and processed foods. Companies are actively seeking innovative psychrophilic enzymes to create more efficient biochemical pathways.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Eva Benesova, Zoran Sucur, Miroslav Tesinsky, Vojtech Spiwok, Petra Lipovova
Summary: Experimental and in silico analysis revealed that β-D-galactosidases from bacteria belonging to Enterobacteriaceae show higher transglycosylation abilities, while other bacterial strains exhibit higher acceptor selectivity. Homology modeling and ligand-docking method can be used to predict and evaluate models of enzymes-transglycosylation products.
Article
Multidisciplinary Sciences
Cristina C. Clement, Angelo D'Alessandro, Sangeetha Thangaswamy, Samantha Chalmers, Raquel Furtado, Sheila Spada, Giada Mondanelli, Federica Ianni, Sarah Gehrke, Marco Gargaro, Giorgia Manni, Luisa Carlota Lopez Cara, Peter Runge, Wanxia Li Tsai, Sinem Karaman, Jorge Arasa, Ruben Fernandez-Rodriguez, Amanda Beck, Antonio Macchiarulo, Massimo Gadina, Cornelia Halin, Francesca Fallarino, Mihaela Skobe, Marc Veldhoen, Simone Moretti, Silvia Formenti, Sandra Demaria, Rajesh K. Soni, Roberta Galarini, Roccaldo Sardella, Gregoire Lauvau, Chaim Putterman, Kari Alitalo, Ursula Grohmann, Laura Santambrogio
Summary: 3-hydroxy-L-kynurenamine (3-HKA) is a metabolite deriving from a lateral pathway of tryptophan catabolism. The authors identify 3-HKA as a biogenic amine with anti-inflammatory properties, showing its protective effects against psoriasis and nephrotoxic nephritis in mice.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Zexi Shao, Na Liu, Wei Wang, Lizhong Zhu
Summary: This study revealed the metabolic and transcriptional responses of rice to organic pollutants, providing a new reference for comprehensive evaluation of their environmental risks.
ENVIRONMENTAL POLLUTION
(2022)
Review
Biotechnology & Applied Microbiology
Shan Wang, Hai Deng
Summary: The incorporation of beta-hydroxy-alpha-amino acids (beta HAAs) into organic molecules is important in various fields, but controlling the two chiral centers remains challenging. Biocatalytic approaches are needed to selectively introduce these chiral centers due to the limitations of current methods. Future developments may involve expanding enzyme substrate scopes and engineering enzymes for enhanced conversion and stereoselectivity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Jelena Bozunovic, Milica Milutinovic, Neda Anicic, Marijana Skoric, Dragana Matekalo, Suzana Zivkovic, Milan Dragicevic, Biljana Filipovic, Tijana Banjanac, Luka Petrovic, Danijela Misic
Summary: Secoiridoid glucosides (SGs) are bitter tasting monoterpenoids that play a key role in plant defense. This study found that SGs can undergo hydrolysis mediated by enzymes, leading to different metabolic pathways in plants. Transient overexpression of two beta-D-glucosidases can alter the metabolite profiles of SGs, potentially impacting the overall phytochemistry of the plant.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Physical
Wenlong Zheng, Haoran Yu, Sai Fang, Kaitong Chen, Zhe Wang, Xiuli Cheng, Gang Xu, Lirong Yang, Jianping Wu
Summary: The study utilized a CAST/ISM strategy to accelerate the directed evolution of LTA by focusing on amino acid residues within the substrate pocket. Significant progress was made in the synthesis of products, showing promise for industrial applications.
Article
Nutrition & Dietetics
Piotr Kaczka, Katarzyna Kubicka, Amit Batra, Marcin Maciejczyk, Edyta Kopera, Justyna Bira, Tomasz Zajac
Summary: This study aimed to investigate the effect of simultaneous supplementation of beta-hydroxy-beta-methylbutyrate and L-Arginine alpha-ketoglutarate on lower limb power and muscle damage in medium distance runners aged 15.3 years old. The results suggested that the supplement combination may help prevent a reduction in jump performance during training, but showed no influence on markers of muscle damage or mood.
Article
Chemistry, Applied
Yusuke Matsumoto, Yukiko Enomoto, Satoshi Kimura, Tadahisa Iwata
Summary: The study shows that 1,3-alpha-D-glucan and LMW-1,3-beta-D-glucan hydrogels can be compressed and recover their original shape, while HMW-1,3-beta-D-glucan hydrogels have better deformability and shape recovery.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Organic
Hyun Sun Jang, Sung-Gon Kim
Summary: An organocatalytic asymmetric [3 + 3]-cycloaddition has been developed for the enantioselective synthesis of functionalized morpholin-3-ones. The reaction is tolerant to various substrates, providing the desired products in moderate yields with good-to-excellent enantioselectivities.
TETRAHEDRON LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Cuicui Liu, Songling Yan, Jing Zhao, Miao Lin, Baiyun Duan, Zhenqing Zhang, Yao Yang, Zhonghua Liu, Sheng Yuan
Summary: An endo-beta-1,3(4)-glucanase AnENG16A from Aspergillus nidulans exhibits distinctive catalytic features, producing 3-O-beta cellobiosyl-D-glucose and 3-O-beta gentiobiosyl-D-glucose with high antioxidant activity. The linkage type at the non-reducing end of beta-glucotrioses may enhance antioxidant activity compared to beta-1,3-linkage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nutrition & Dietetics
Isabel Cornejo-Pareja, Maria Ramirez, Maria Camprubi-Robles, Ricardo Rueda, Isabel Maria Vegas-Aguilar, Jose Manuel Garcia-Almeida
Summary: The study demonstrates the positive impact of a high-calorie, high-protein oral nutritional supplement with HMB on improving nutritional status, increasing body weight, and muscle-related parameters, especially in enhancing muscle health.
Article
Spectroscopy
Fan Huang, Shulei Zhuang, Weiwei Liu, Lie Lin, Lu Sun
Summary: The study used dispersion corrected density functional theory (DFT-D) calculations to investigate the complex formed by beta-cyclodextrin and D/L-penicillamine. It was found that the interaction energy between L-Pen and beta-CD is lower than that between D-Pen and beta-CD. Vibration frequency analysis showed that the skeleton vibration and carboxyl group vibration were significant spectral standards for distinguishing the complexes. Topological analysis revealed the differences in hydrogen bonds and van der Waals interactions between beta-CD-D-Pen and beta-CD-L-Pen.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biotechnology & Applied Microbiology
Jie Ren, Wenzhu Tang, Caleb Don Barton, Owen M. Price, Mark Wayne Mortensen, Alexandra Phillips, Banner Wald, Simon Elgin Hulme, Logan Powell Stanley, Joan Hevel, Jixun Zhan
Summary: The study discovered a novel glycosyltransferase gene and demonstrated its ability to glycosylate various substrates through in vitro enzymatic reactions. The enzyme showed flexibility in different hosts and efficiently produced valuable glycosides, providing a promising method for biocatalysis and production of natural products.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Organic
Hong-Yao Wang, Atsushi Kato, Kyoko Kinami, Yi-Xian Li, George W. J. Fleet, Chu-Yi Yu
ORGANIC & BIOMOLECULAR CHEMISTRY
(2016)
Article
Chemistry, Organic
Zilei Liu, Akihide Yoshihara, Sarah F. Jenkinson, Mark R. Wormald, Ramon J. Estevez, George W. J. Fleet, Ken Izumori
Article
Chemistry, Inorganic & Nuclear
Akiko Kamori, Atsushi Kato, Shota Miyawaki, Junna Koyama, Robert J. Nash, George W. J. Fleet, Daisuke Miura, Fumihiro Ishikawa, Isao Adachi
TETRAHEDRON-ASYMMETRY
(2016)
Article
Chemistry, Organic
Atsushi Kato, Izumi Nakagome, Shinpei Nakagawa, Kyoko Kinami, Isao Adachi, Sarah F. Jenkinson, Jerome Desire, Yves Bleriot, Robert J. Nash, George W. J. Fleet, Shuichi Hirono
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Chemistry, Inorganic & Nuclear
Amber L. Thompson, Sarah F. Jenkinson, George W. J. Fleet
TETRAHEDRON-ASYMMETRY
(2017)
Review
Biochemistry & Molecular Biology
Kate Prichard, David Campkin, Nicholas O'Brien, Atsushi Kato, George W. J. Fleet, Michela I. Simone
CHEMICAL BIOLOGY & DRUG DESIGN
(2018)
Review
Chemistry, Organic
Adam Wood, Kate L. Prichard, Zane Clarke, Todd A. Houston, George W. J. Fleet, Michela I. Simone
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
R. Fernando Martinez, Sarah F. Jenkinson, Shinpei Nakagawa, Atsushi Kato, Mark R. Wormald, George W. J. Fleet, Jackie Hollinshead, Robert J. Nash
Article
Biochemistry & Molecular Biology
Atsushi Kato, Junna Koyama, Kenta Shinzawa, Shuki Imaeda, Isao Adachi, Robert J. Nash, George W. J. Fleet, Megumi Shintani, Chihiro Takeuchi, Fumihiro Ishikawa
BIOORGANIC & MEDICINAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Akihide Yoshihara, Hirofumi Sakoguchi, Tomoya Shintani, George W. J. Fleet, Ken Izumori, Masashi Sato
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Zilei Liu, Sarah F. Jenkinson, Akihide Yoshihara, Mark R. Wormald, Ken Izumori, George W. J. Fleet
Article
Biochemistry & Molecular Biology
Qing-Kun Wu, Kyoko Kinami, Atsushi Kato, Yi-Xian Li, George W. J. Fleet, Chu-Yi Yu, Yue-Mei Jia
Article
Chemistry, Organic
Zilei Liu, Akihide Yoshihara, Sarah F. Jenkinson, Mark R. Wormald, Ciaran Kelly, John T. Heap, Mikkel H. S. Marqvorsen, Ramon J. Estevez, George W. J. Fleet, Shinpei Nakagawa, Ken Izumori, Robert J. Nash, Atsushi Kato
Article
Biochemistry & Molecular Biology
Olumayokun A. Olajide, Victoria U. Iwuanyanwu, Owolabi W. Banjo, Atsushi Kato, Yana B. Penkova, George W. J. Fleet, Robert J. Nash
Summary: This study found that idoBR1, a cucumber-derived iminosugar amino acid, could dose-dependently reduce the inflammatory responses in LPS-activated microglia cells. These findings suggest that idoBR1 may have anti-inflammatory effects and could be further investigated for its potential in other neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Shota Miyawaki, Yuki Hirokami, Kyoko Kinami, Masako Hoshino, Daisuke Minehira, Daiki Miyamoto, Robert J. Nash, George W. J. Fleet, Isao Adachi, Naoki Toyooka, Atsushi Kato
BIOORGANIC & MEDICINAL CHEMISTRY
(2017)