Article
Chemistry, Multidisciplinary
Hiromasa Matsuda, Erina Yoshida, Takaaki Shinoda, Kazuki Sato, Rintaro Iwata Hara, Takeshi Wada
Summary: Recent advances in nucleic acid therapeutics have increased the demand for efficient methods to chemically synthesize oligodeoxyribonucleotides (ODNs). This study introduces a new approach using the H-phosphonate method and N-unprotected 5'-phosphite monomers to achieve the synthesis of ODNs, including TTT and tetramers with four different nucleobases.
Article
Multidisciplinary Sciences
Taiki Tsurusaki, Kazuki Sato, Hiroki Imai, Kunihiro Hirai, Daisuke Takahashi, Takeshi Wada
Summary: Phosphorodiamidate morpholino oligonucleotides (PMOs) are a promising type of antisense oligonucleotides, but their challenging synthesis makes them difficult to access. This research presents an efficient synthetic approach for PMOs using the H-phosphonate approach. The use of phosphonium-type condensing reagents significantly reduced coupling times compared with the current synthetic approach. This approach would facilitate the large-scale synthesis of PMOs and accelerate their popularity and accessibility as a next-generation therapy.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xinyue Wang, Qiang Wang, Ping Wang, Man Zhou, Bo Xu, Ying Liu, Yuanyuan Yu
Summary: Chitosan was modified with thioctic acid to prepare soft films with improved physical-mechanical, antibacterial, and thermal properties. The modified films showed higher flexibility, good antibacterial activity, and resistance to harsh conditions, making them suitable for flexible devices and packaging.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Alessandra Pacini, Daniele Tomassoni, Elena Trallori, Laura Micheli, Francesco Amenta, Carla Ghelardini, Lorenzo Di Cesare Mannelli, Enea Traini
Summary: Both animal studies and clinical trials have shown that the (+)-enantiomer of thioctic acid has better effects in relieving pain, protecting peripheral nerves, and reducing oxidative stress.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biophysics
Hui Liu, Zhiwei Deng, Tianhao Li, Jiaqi Bu, De Wang, Jiahui Wang, Meng Liu, Jiacheng Li, Yanjing Yang, Shian Zhong
Summary: A novel poly(lipoic acid)-poly(ethylene glycol) hydrogel with glutathione responsiveness was developed for drug delivery in tumor microenvironment, showing promising applications as an intelligent drug delivery system for cancer treatment.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Zachary J. Struzik, Shruti Biyani, Tim Grotzer, Judith Storch, David H. Thompson
Summary: In this study, a robust pathway to optically pure PG is presented, utilizing triethyl ammonium H-phosphonate salt, instead of the currently unavailable commercially available optically pure material. The challenges in purification during the process have been addressed.
Article
Chemistry, Multidisciplinary
Chen Chen, Xiao Yang, Shu-jing Li, Chao Zhang, Yu-ning Ma, Yu-xia Ma, Peng Gao, Shu-zhong Gao, Xiao-jun Huang
Summary: The TATA supramolecular hydrogel, synthesized through ring-opening polymerization, possesses self-healing and injectable properties, and has been successfully used for skin wound healing and burn wound infection treatment. XPS confirmed successful intermolecular crosslinking, showcasing its potential as a surgical antibacterial bioadhesive.
Article
Multidisciplinary Sciences
Yoshiaki Masaki, Yukiko Onishi, Kohji Seio
Summary: Substitutions, insertions, and deletions derived from synthetic oligonucleotides are challenges for the synthesis of long DNA. We quantified these synthetic errors and found that the quality of the final product depends on the conditions of solid phase chemical synthesis. Non-canonical nucleosides resistant to side reactions can be used to reduce the error rate of specific substitutions.
SCIENTIFIC REPORTS
(2022)
Article
Polymer Science
Jinwei Cui, Xuesu Su, Bining Jiao, Yueting Liao, Wei Xiang, Yangyang Fang
Summary: Thioctic acid (TA), a natural substance, is an inexpensive raw material for high-performance polymers due to its simple structure. Progress has been made in the research of TA-based materials, especially in the field of self-healing materials. Through reversible disulfide bonds, the obtained polymer can be easily and effectively recovered, indicating that TA-based polymers could potentially become low-cost, high-performance, and multi-functional sustainable materials in the future. Combining nanomaterials and computer simulation, TA-based materials present a fresh approach to future research.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Chemistry, Physical
Jotiram N. Malegaonkar, Mohammad Al Kobaisi, Prabhat K. Singh, Sidhanath V. Bhosale, Sheshanath V. Bhosale
Summary: The derivatisation of tetraphenylethylene is widely used to synthesize molecular sensor for detecting explosives and other analytes, but its effects have been poorly studied. In this study, we report the detection of various nitro phenols using fluorescence signals produced by TPPE. The fluorescence of TPPE at 411 nm and 432 nm is quenched in the presence of TNP through electron transfer mechanism. Furthermore, TPPE shows strong binding affinity and selectivity towards TNP over other nitroaromatic analytes, with a sensing detection limit of 4.58 x 10-9 M. Detailed molecular simulation experiments support the sensing mechanism and provide insight into the interaction between TPPE and TNP.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Agricultural Engineering
Penghao Sun, Yuqi Wang, Zhen Huang, Xinxin Yang, Fuhao Dong, Xu Xu, He Liu
Summary: The healable smart electronic sensor, made from a polymeric material, displayed long-term stability, moderate strain, high adhesive strength, and self-healing capacity. The material also exhibited conductivity sensitivity and antimicrobial activity. It showed great potential for detecting human movement.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Engineering, Biomedical
Chunxiao Chai, Pengfei Zhang, Lin Ma, Qi Fan, Zhicheng Liu, Xiang Cheng, Yunpeng Zhao, Weiwei Li, Jingcheng Hao
Summary: In this study, regenerative antibacterial hydrogels (RAH) containing in situ synthesized silver nanoparticles (AgNPs) were prepared using thioctic acid. RAH showed regenerative, self-healing, and injectable ability, and could be stored as powder. RAH exhibited antioxidant properties and extraordinary antimicrobial capacities against MDR bacteria, making it a promising therapy for chronic diabetic wounds.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Juan Canoura, Obtin Alkhamis, Yingzhu Liu, Connor Willis, Yi Xiao
Summary: This study presents a high-throughput method for quantifying the binding affinity, specificity, and cross-reactivity of aptamers using exonuclease digestion kinetics. It was used to identify new aptamers for fentanyl and its analogs and to validate the results. This approach greatly speeds up the characterization process of aptamers and streamlines sensor development.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Materials Science, Textiles
Xinyi Hu, Que Kong, Rong Li, Change Zhou, Zhiguang Li
Summary: In this study, a multifunctional hydrogel with excellent rheology, antibacterial and antioxidant properties was successfully prepared. The hydrogel is capable of slowly absorbing wound tissue fluid and maintaining wound moisture. By modifying the properties of the hydrogel, it could be used as an ideal wound dressing.
FIBERS AND POLYMERS
(2023)
Article
Materials Science, Textiles
Xinyi Hu, Que Kong, Rong Li, Change Zhou, Zhiguang Li
Summary: Developing an ideal wound dressing with desirable properties such as biocompatibility, adhesive property, antibacterial property, and simple production is of great significance. In this study, multifunctional tannic-thioctic acid (TATA) hydrogels with excellent rheological properties were prepared using polyphenol-sulfur radical crosslinking. The TATA hydrogel showed potential for various medical applications as a novel wound dressing.
FIBERS AND POLYMERS
(2023)
Article
Chemistry, Multidisciplinary
Richard H. Huang, Nazia Nayeem, Ye He, Jorge Morales, Duncan Graham, Rafal Klajn, Maria Contel, Stephen O'Brien, Rein Ulijn
Summary: The study demonstrates the use of zwitterionic tetrapeptide modalities to direct nanoparticle assembly under physiological conditions, allowing for the reduction of cell growth in cancer cells through enzyme-responsive self-assembly. Customizing the peptide sequence can program the enzyme responsiveness and cellular uptake pathway.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Matthew E. Berry, Samantha M. McCabe, Neil C. Shand, Duncan Graham, Karen Faulds
Summary: A model for predicting the depth of surface enhanced Raman scattering (SERS) active nanotags embedded within tissue is demonstrated using ratiometric analysis. This study utilized a handheld spatially offset Raman (SORS) instrument to detect SESORS signals from nanotags at depths down to 48 mm for the first time using a backscattering SORS geometry.
CHEMICAL COMMUNICATIONS
(2022)
Article
Spectroscopy
Hannah Carruthers, Don Clark, Fiona C. Clarke, Karen Faulds, Duncan Graham
Summary: Chemical mapping by Raman spectroscopy is commonly used in the pharmaceutical industry to analyze component distribution within tablets, but current methods are limited to examining the sample's exposed surface. Recent research has explored a combination of Raman spectroscopic mapping and serial sectioning as an alternative method to obtain depth profiles of samples, providing appropriate lateral and axial resolution through the use of independent sample sectioning alongside Raman mapping.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Avigail Baruch Leshem, Sian Sloan-Dennison, Tlalit Massarano, Shavit Ben-David, Duncan Graham, Karen Faulds, Hugo E. Gottlieb, Jordan H. Chill, Ayala Lampel
Summary: Inspired by the role of intracellular liquid-liquid phase separation (LLPS) in the formation of membraneless organelles, researchers have developed dynamic compartments formed by LLPS of intrinsically disordered proteins (IDPs) or short peptides. However, the molecular mechanisms underlying the formation of biomolecular condensates have not been fully understood. In this study, the authors demonstrate that the LLPS propensity, dynamics, and encapsulation efficiency of designed peptide condensates can be tuned by subtle changes to the peptide composition. This sequence-structure-function correlation could pave the way for the future development of compartments for various applications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Neville Murphy, William J. Tipping, Henry J. Braddick, Liam T. Wilson, Nicholas C. O. Tomkinson, Karen Faulds, Duncan Graham, Pau Farras
Summary: This study reports the application of spectral phasor analysis to unmix three bioorthogonal Raman probes in live cells. Triplex detection of a metallacarborane, a bis-alkyne, and a deuterated fatty acid can be achieved within the cell-silent region of the Raman spectrum. Additionally, nine regions of interest can be spectrally unmixed from the hyperspectral dataset, offering a new capability for multiplexed Raman imaging of live cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Samantha M. M. McCabe, Gregory Q. Q. Wallace, Sian Sloan-Dennison, William J. J. Tipping, Neil C. C. Shand, Duncan Graham, Marie Boyd, Karen Faulds
Summary: This study investigates the uptake and distribution of silica coated gold nanoparticles (AuNP-SHINs) in three-dimensional multicellular tumour spheroids derived from glioblastoma cells. The results show that the localization of nanoparticles within the spheroids is influenced by factors such as size, quiescence, and hypoxia.
Article
Chemistry, Multidisciplinary
Aristea Anna Leventi, Henry J. Braddick, Kharmen Billimoria, Gregory Q. Wallace, Heidi Goenaga-Infante, Nicholas C. O. Tomkinson, Karen Faulds, Duncan Graham
Summary: This article reports the synthesis of nanocarriers for delivering the antitumor drug cisplatin. Multimodal imaging techniques, including surface enhanced Raman scattering and laser ablation inductively coupled plasma time of flight mass spectrometry, were used to visualize the intracellular uptake of both the nanocarrier and drug.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Iona E. Hill, Marie Boyd, Kirsty Milligan, Cerys A. Jenkins, Annette Sorensen, Andrew Jirasek, Duncan Graham, Karen Faulds
Summary: Radiation therapy is widely used in cancer treatment, but there are large variations in treatment responses due to the heterogeneity of cancer. This study evaluated the potential of Raman spectroscopy for monitoring cellular response to radiation in specific cell cycle phases. The results showed that Raman spectroscopy combined with cell cycle and DNA damage analysis could discriminate the subtle cellular changes associated with radiation response.
Article
Chemistry, Analytical
Yichen Zhao, Nikesh Patel, Peihuan Sun, Karen Faulds, Duncan Graham, Juewen Liu
Summary: Due to their programmable structures, aptamers can retain their target binding function even when split into two halves. Comparisons between split aptamers and full-length aptamers showed that split aptamers with longer stem lengths had similar dissociation constants to the full-length aptamer, while a shorter stem construct had a much higher dissociation constant. Temperature-dependent fluorescence measurements and isothermal titration calorimetry confirmed the lower thermodynamic stability of split aptamers.
Article
Chemistry, Analytical
Yichen Zhao, Nikesh Patel, Peihuan Sun, Karen Faulds, Duncan Graham, Juewen Liu
Summary: Split aptamers, which can be divided into two halves while retaining their target binding function, have been widely used in biosensors. However, there is still a lack of fundamental studies on their binding. In this study, a new aptamer called OTC5 was used to compare different split aptamers with the full-length aptamer. It was found that split aptamers with longer stem lengths showed similar binding properties to the full-length aptamer, while a shorter stem construct had a significantly lower binding affinity. Temperature-dependent fluorescence measurements and isothermal titration calorimetry further confirmed the differences in stability and binding thermodynamics of split aptamers.
Article
Biochemistry & Molecular Biology
William J. Tipping, Andrew S. Merchant, Rebecca Fearon, Nicholas C. O. Tomkinson, Karen Faulds, Duncan Graham
Summary: Stimulated Raman scattering (SRS) microscopy is a powerful technique for visualizing the uptake and distribution of drugs and small molecules in live cells. It can directly detect bioactive molecules and has potential for preclinical drug development. Real-time detection of drug-cell interactions allows for the assessment of drug uptake and distribution, providing important insights for drug development.
RSC CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Alexandre Verdin, Sian Sloan-Dennison, Cedric Malherbe, Duncan Graham, Gauthier Eppe
Summary: In this study, we successfully achieved high specific targeting and measurement of folate receptor alpha (FRα) in cancer cells by building nanotags based on nanoparticles. The PERS response of the nanotags was positively correlated with the expression level of FRα, enabling the discrimination of different expression levels of cancer cells. Moreover, due to the biocompatibility of the nanotags, measurements could be performed on live cells, providing flexibility for experimental conditions.
Article
Chemistry, Analytical
Hannah Carruthers, Don Clark, Fiona C. Clarke, Karen Faulds, Duncan Graham
Summary: Vibrational spectroscopic chemical imaging is an important tool in the pharmaceutical industry for characterizing the spatial distribution of components in drug products. However, the long data acquisition times have limited its applicability. This study explores a laser direct infrared chemical imaging approach that provides high-definition images at a much faster rate compared to traditional techniques.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Kiatnida Treerattrakoon, Pimporn Roeksrungruang, Tararaj Dharakul, Deanpen Japrung, Karen Faulds, Duncan Graham, Suwussa Bamrungsap
Summary: This article describes the use of SERS tags and magnetic separation for the detection of miR-29a, a biomarker for cancers. The assay utilizes capture and detection probes, along with gold nanorods and magnetic nanoparticles, to facilitate hybridization and sandwich complex formation. The resultant complexes are then enriched and analyzed using SERS. The method demonstrates high sensitivity and selectivity for miR-29a detection.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Multidisciplinary
William J. Tipping, Liam T. Wilson, Connie An, Aristea A. Leventi, Alastair W. Wark, Corinna Wetherill, Nicholas C. O. Tomkinson, Karen Faulds, Duncan Graham
Summary: Statins have shown potential anti-tumour activity in breast cancer treatment, but understanding their mode of action and accurately assessing their efficacy is challenging. This study demonstrates the use of stimulated Raman scattering (SRS) microscopy for characterising breast cancer cells treated with statins, revealing different lipid accumulation phenotypes associated with cell viability.
Article
Chemistry, Organic
Geeta Devi Yadav, Pooja Chaudhary, Balaram Pani, Surendra Singh
Summary: Chiral transition metal complexes with privileged ligands are efficient catalysts for various asymmetric organic transformations. Transition metal complexes of C1-symmetric pyrrolidine-based ligands have been widely used in asymmetric organic reactions. However, a comprehensive review article on the transition metal complexes of chiral C1-symmetric pyrrolidine-based ligands derived from (L)-proline has not been published.
TETRAHEDRON LETTERS
(2024)
