Article
Chemistry, Organic
Harbansh Singla, Jyotirmoy Maity, Sandeep Kumar, Kavita Kavita, Riya Chaudhary, Ashok K. K. Prasad
Summary: A convergent route has been developed for the synthesis of a new class of bicyclic nucleosides. The synthesis of arabino-configured uracil and thymine bicyclic nucleosides was achieved in 24% and 27% overall yields, respectively, starting from 1,2,5,6-di-O-isopropylidene-α-D-glucofuranose. Important steps in this synthetic protocol include Vorbruggen base coupling and chemo-enzymatic regioselective acetylation using Lipozyme (R) TL IM, which was found to be recoverable and reusable without losing its selectivity.
SYNTHESIS-STUTTGART
(2023)
Article
Biochemistry & Molecular Biology
Harbansh Singla, Sandeep Kumar, Jyotirmoy Maity, Ashok K. Prasad
Summary: A greener chemo-enzymatic methodology has been developed for synthesizing conformationally restricted diastereomeric AZT analogues. The primary hydroxyl group of 3'-azido-3'-deoxy-beta-D-glucofuranosyl pyrimidines was acetylated using Novozyme (R) 435, and then converted to the desired bicyclic AZT analogue.
CARBOHYDRATE RESEARCH
(2023)
Article
Multidisciplinary Sciences
Tao Cai, Hongbing Sun, Jing Qiao, Leilei Zhu, Fan Zhang, Jie Zhang, Zijing Tang, Xinlei Wei, Jiangang Yang, Qianqian Yuan, Wangyin Wang, Xue Yang, Huanyu Chu, Qian Wang, Chun You, Hongwu Ma, Yuanxia Sun, Yin Li, Can Li, Huifeng Jiang, Qinhong Wang, Yanhe Ma
Summary: Researchers have developed an artificial starch anabolic pathway (ASAP) in a cell-free system for starch synthesis from CO2 and hydrogen, achieving a significantly higher rate than traditional methods. This approach paves the way for future chemo-biohybrid starch synthesis from CO2.
Article
Chemistry, Organic
Sandeep Kumar, Jyotirmoy Maity, Banty Kumar, Sumit Kumar, Ashok K. Prasad
Summary: Conformationally restricted diastereomeric homoarabinofuranosylpyrimidines (AZT analogue) were synthesized using chemoenzymatic and chemical routes. The chemoenzymatic method showed superiority in terms of synthetic steps and overall yield.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Tianzhen Li, Zijian Tan, Zijing Tang, Pi Liu, Haifeng Liu, Leilei Zhu, Yanhe Ma
Summary: An efficient method for synthesizing glycolic acid was reported in this study, utilizing a catalytic C-C coupling reaction of formaldehyde and selective oxidation, with an evolved enzyme showing improved activity at high formaldehyde concentrations.
Article
Biochemistry & Molecular Biology
Bing-Mei Su, Yi-Bing Shi, Wei Lin, Lian Xu, Xin-Qi Xu, Juan Lin
Summary: A novel chemo-enzymatic process was developed to produce highly purified DHEA, an important neurosteroid hormone, in the pharmaceutical industry.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wei He, Jiacheng Ni, Yu-Cai He, Jianren Ye
Summary: Efficient synthesis of furan-based chemicals from biomacromolecule using chemoenzymatic approaches has been developed in this study. Different biomass sources can produce varying concentrations of furfural, and the conversion of furfural to furfurylamine is inhibited by by-products and soluble sugars produced during the biomass conversion. The amination of furfural to furfurylamine can be achieved by using specific enzymes. This study successfully developed a chemoenzymatic strategy for the conversion of biomacromolecules into valuable furan-based products in an environmentally friendly system.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Amanda J. Reid, Katelyn M. Erickson, Joseph M. Hazel, Vinita Lukose, Jerry M. Troutman
Summary: Complex poly-and oligosaccharides on bacteria's surface can be used for the development of therapeutics or sensors that are selective for specific glycans. The addition of a chemically functional benzylazide tag to polyisoprenoids enables the chemoenzymatic preparation and immobilization of bacterial glycans. This method has been demonstrated to assemble Campylobacter jejuni N-linked heptasaccharide and immobilize it on magnetic beads, showing potential for the discovery of new glycan-interacting partners.
Article
Chemistry, Organic
Beata Zdun, Tamara Reiter, Wolfgang Kroutil, Pawel Borowiecki
Summary: We developed chemoenzymatic routes using low-cost starting materials and enzyme preparations to synthesize tenofovir. The key step involved stereoselective reduction or kinetic resolution using alcohol dehydrogenase or lipase as biocatalysts. By employing immobilized lipase in a mixture of vinyl acetate and toluene, the desired (R)-ester was obtained with high yield and optical purity. Alternatively, reduction of a ketone using lyophilized E. coli cells containing recombinant alcohol dehydrogenase resulted in excellent conversion and yield of the corresponding (R)-alcohol. The enzymatic strategy could be applied in the synthesis of tenofovir prodrug derivatives.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Alexander Rittner, Mirko Joppe, Jennifer J. Schmidt, Lara Maria Mayer, Simon Reiners, Elia Heid, Dietmar Herzberg, David H. Sherman, Martin Grininger
Summary: This study reports a strategy to introduce fluorine into complex polyketides during biosynthesis. By exchanging a domain of the polyketide synthase, the ability to incorporate fluorine during polyketide chain extension was achieved. The results demonstrate the potential application of this method in the chemoenzymatic synthesis of fluorinated compounds.
Article
Chemistry, Organic
David Leparfait, Feng Xiao, Delphine Coupri, Sabrina Gueulle, Florie Desriac, Aurelie Budin-Verneuil, Nicolas Verneuil, Axel Hartke, Emmanuel Pfund, Thierry Lequeux
Summary: Tri- and tetra-substituted alkenes, including fluoroalkenes, were obtained via selective ring-opening reaction of functionalized oxetanes. By introducing adenine nucleic base, alkenyl and fluoroalkenyl derivatives were synthesized as potential nucleoside mimics and evaluated for their activity against DltA enzyme.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Organic
Evan O. Romero, Jonathan C. Perkins, Jessica E. Burch, David A. Delgadillo, Hosea M. Nelson, Alison R. H. Narayan
Summary: The first asymmetric synthesis of the pentacyclic fungal marine natural product Xyloketal B is described using a chemoenzymatic strategy. The approach utilizes a biocatalytic benzylic hydroxylation and a [4 + 2] cycloaddition to access key intermediates enroute to (+)-xyloketal B. MicroED is used to confirm the relative configuration of an intermediate, and a reductive approach is employed to access a second key intermediate, culminating in the stereoselective synthesis of (+)-xyloketal B.
Article
Biochemistry & Molecular Biology
Sergio Jurado, Beatriz Dominguez-Perez, Ona Illa, Jan Balzarini, Felix Busque, Ramon Alibes
Summary: The enantioselective preparation of the two isomers of 4-hydroxy-2-cyclohexanone derivatives was achieved through asymmetric transfer hydrogenation reactions. A stereoselective route to a cytosine analogue was described, showing selectivity for herpes simplex virus TKs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Zewei Yao, Mao Li, Yao Sun, Chaozhan Wang, Yinmao Wei
Summary: This study developed a restricted-access boronate affinity mesoporous adsorbent with high binding capacities for small cis-diol-containing molecules. The adsorbent selectively extracts cis-diols from biosamples while excluding proteins completely. The adsorbent was successfully used in analyzing nucleosides and cis-diol drugs in human serum, simplifying the analysis process.
Article
Biochemistry & Molecular Biology
Mikhail S. Drenichev, Vladimir E. Oslovsky, Anastasia A. Zenchenko, Claudia V. Danilova, Mikhail A. Varga, Roman S. Esipov, Dmitry D. Lykoshin, Cyril S. Alexeev
Summary: A comparative analysis was conducted on the transglycosylation conditions catalyzed by E. coli nucleoside phosphorylases, leading to the formation of 2'-deoxynucleosides. The study demonstrated that maximal yields of modified 2'-deoxynucleosides can be achieved with a slight excess of carbohydrate residue donor nucleoside and a four-fold lack of phosphate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Jerome Wintzer-Wehekind, Eric Horlick, Reda Ibrahim, Asim N. Cheema, Marino Labinaz, Najaf Nadeem, Mark Osten, Melanie Cote, Josep Ramon Marsal, Donald Rivest, Alier Marrero, Christine Houde, Josep Rodes-Cabau
Summary: Adding clopidogrel to aspirin for 3 months after transcatheter atrial septal defect (ASD) closure reduces the incidence of new-onset migraine attacks. Following clopidogrel cessation at 3 months, the incidence and severity of migraine attacks progressively decrease over time, with most patients experiencing improvement or resolution within 6 to 12 months of follow-up.
Article
Cardiac & Cardiovascular Systems
Bruno Garcia del Blanco, Imanol Otaegui, Jose F. Rodriguez-Palomares, Antoni Bayes-Genis, Eduard Fernandez-Nofrerias, Victoria Vilalta del Olmo, Xavier Carrillo, Borja Ibanez, Fernando Worner, Juan Casanova, Eva Pueo, Jose R. Gonzalez-Juanatey, Javier Lopez-Pais, Alfredo Bardaji, Gil Bonet, Monica Fuertes, Antonio Rodriguez-Sinovas, Marisol Ruiz-Meana, Javier Inserte, Ignasi Barba, Sandra Gomez-Talavera, Gerard Marti, Bernat Serra, Neus Bellera, Manuel Ojeda-Ramos, Hug Cuellar, Filipa Valente, Maria Angeles Carmona, Elisabet Miro-Casas, Josep R. Marsal, Antonia Sambola, Rosa M. Lidon, Jordi Baneras, Jaime Elizaga, Ferran Padilla, Jose A. Barrabes, Derek J. Hausenloy, Ignacio Ferreira-Gonzalez, David Garcia-Dorado
Summary: In patients undergoing primary percutaneous coronary intervention (PPCI) for ST-segment elevation myocardial infarction (STEMI), neither remote ischemic conditioning (RIC) nor the GLP-1 analog exenatide, alone or in combination, were able to reduce infarct size (IS).
BASIC RESEARCH IN CARDIOLOGY
(2021)
Article
Medicine, General & Internal
Gloria Longinotti, Gabriel Ybarra, Susana Vighi, Claudia Perandones, Javier Montserrat, Juan Sebastian Yakisich, Mariano Grasselli, Martin Radrizzani
Summary: This article introduces a method using two synthetic molecules to standardize the detection of protein markers in histological sections. By combining aptamers and G-quadruplex, this method enables reproducible results in staining cancer markers in histological sections, offering a synthetic and standardized approach for prognostic marker detection in cancer.
Article
Cardiac & Cardiovascular Systems
Aida Ribera, Emmanuel Gimenez, Gerard Oristrell, Dimelza Osorio, Josep Ramon Marsal, Lidia Garcia-Perez, Monica Ballesteros, Eduard Rodenas, Yassin Belahnech, Roxana Escalona, Nuria Rivas, Ivo Roca-Luque, Ignacio Ferreira-Gonzalez, Mireia Espallargues
Summary: This study evaluated the cost-effectiveness of implantable cardioverter-defibrillators (ICD) for the primary prevention of cardiac arrhythmias. The results showed that ICD systems are cost-effective for patients with ischemic and nonischemic heart disease younger than 68 years old. However, for older nonischemic patients, the cost-effectiveness ratio is higher.
REVISTA ESPANOLA DE CARDIOLOGIA
(2022)
Article
Chemistry, Applied
Edward Valera-Vera, Chantal Reigada, Melisa Saye, Fabio A. Digirolamo, Facundo Galceran, Mariana R. Miranda, Claudio A. Pereira
Summary: The study showed that the anthocyanidin delphinidin inhibits the activity of Trypanosoma cruzi arginine kinase with a non-competitive mechanism. Delphinidin was found to exert trypanocidal activity with low toxicity, making it a potential candidate for the development of drugs for Chagas disease treatment.
NATURAL PRODUCT RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Aida Ribera, Josep R. Marsal, Maria T. Faixedas, Alba Rosas, Helena Tizon-Marcos, Sergio Rojas, Carlos Labata, Merida Cardenas, Silvia Homs, Carlos Tomas-Querol, Joan Garcia-Picart, Gerard Roura, Monica Masotti, Josepa Mauri, Jose Ignacio Pijoan, Jose A. Barrabes, Ignacio Ferreira-Gonzalez
Summary: This study assessed the clinical practice trends and potential impact on 2-year outcomes of drug-eluting stents (DES), ticagrelor, prasugrel, and prolonged dual antiplatelet therapy (DAPT) in patients with ST-segment elevation myocardial infarction (STEMI) who underwent primary percutaneous intervention. Despite the increased use and duration of these treatments between 2010 and 2017, there was no substantial reduction in major cardiovascular outcomes.
REVISTA ESPANOLA DE CARDIOLOGIA
(2022)
Article
Primary Health Care
C. Fuertes Abardia, P. Ballesta Juan, I Cruz Esteve, G. Galindo Ortego, J. R. Marsal Mora, X. Gomez-Arbones
Summary: The study aims to measure the prevalence of potentially inappropriate prescribing (PIP) among the elderly population in Catalonia and evaluate its association with factors such as polypharmacy, gender, age, and sociodemographic conditions. The overall prevalence of PIP is found to be 89.6%, with certain drugs and patient conditions significantly associated with PIP.
MEDICINA DE FAMILIA-SEMERGEN
(2022)
Article
Environmental Sciences
Giselle Berenstein, Enrique A. Hughes, Anita Zalts, Silvana Basack, Sergio M. Bonesi, Javier M. Montserrat
Summary: Phthalic acid esters (PAEs), commonly found in polyethylene covers used in horticultural production units, were found to migrate to soil and atmosphere. The migration of PAEs was influenced by temperature, film thickness, and plastic aging. In addition, PAEs were shown to have an impact on soil biota, reducing cocoon viability and the number of juveniles. Bioindicators exposed to DBP also exhibited changes in enzyme activities.
Article
Environmental Sciences
Soledad Nasello, Erica Beiguel, Gretel Fito-Friedrichs, Carmen Irala, Giselle Berenstein, Silvana Basack, Javier M. Montserrat
Summary: The objective of this study was to evaluate the potential impact of thermal paper tickets used in Argentina as a source of bisphenol A (BPA) on humans and the environment. The study found that thermal paper contains BPA, which can potentially impact human health through contact and ingestion. BPA from thermal paper can also migrate into water and soil, posing a potential environmental contamination.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Claudia Alvarez-Martin, Aida Ribera, Josep Ramon Marsal, Albert Ariza-Sole, Santiago Perez-Hoyos, Gerard Oristrell, Toni Soriano-Colome, Rafael Romaguera, Jose Ignacio Pijoan, Rosa M. Lidon, Josepa Mauri, Ignacio Ferreira-Gonzalez
Summary: During the COVID-19 pandemic, there was a significant decline in urgent cardiovascular hospital admissions, which was attenuated during the second wave. The decline and attenuation of the effect were similar in all subgroups regardless of age, sex, or socioeconomic status.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Medicine, Research & Experimental
Josep Ramon Marsal, Iratxe Urreta-Barallobre, Marimar Ubeda-Carrillo, Dimelza Osorio, Blanca Lumbreras, David Lora, Borja M. Fernandez-Felix, Gerard Oristrell, Eduard Rodenas-Alesina, Lorena Herrador, Monica Ballesteros, Javier Zamora, Jose Pijoan, Aida Ribera, Ignacio Ferreira-Gonzalez
Summary: This study investigates the impact of the degree of association between death and AMI on the sample size requirement of trials including MACE. The results show that variations in the degree of association can lead to significant changes in the sample size requirement of the trial.
Article
Medicine, General & Internal
Silvia Sola-Munoz, Jorge Morales, Xavier Jimenez-Fabrega, Sonia Jimenez-Delgado, Youcef Azeli, J. Ramon Marsal, Sara Jordan, Josepa Mauri, Javier Jacob
Summary: The objective of this study was to develop and validate a risk scale (MARIACHI) to identify patients at an increased risk of mortality in non-ST-segment elevation acute coronary syndrome (NSTEACS) in a prehospital setting. The model, including age, systolic blood pressure, heart rate, Killip-Kimball classification, and ST depression, showed good discrimination and calibration in predicting high-risk NSTEACS patients. This scale could assist in treatment decisions and referrals at the prehospital level.
INTERNAL AND EMERGENCY MEDICINE
(2023)
Article
Medicine, General & Internal
Marta Leston Vazquez, Carles Vilaplana-Carnerero, Ainhoa Gomez-Lumbreras, Oriol Prat-Vallverdu, Josep Ramon Marsal, Cristina Vedia Urgell, Maria Giner-Soriano, Rosa Morros
Summary: This study developed an algorithm to identify pregnancy episodes in Catalonia, Spain and described the drugs used during gestation. The findings showed that out of 327,865 pregnancy episodes, 83.4% were exposed to at least one drug, with supplements, analgesics, NSAIDs, and antibiotics being the most commonly used ones.
