Journal
TALANTA
Volume 110, Issue -, Pages 8-14Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2013.03.022
Keywords
Acetylcholine-selective electrode; Enzymatic assay; Acetylcholinesterase inhibitors; Galantamine determination; Anti-Alzheimer's drugs
Categories
Funding
- Ministerio de Ciencia y Tecnologia, Spain [CTQ2011-27049]
- University of Murcia
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A new kinetic-potentiometric method for the characterization and analytical determination of competitive reversible enzyme inhibitors was developed. The method is based on a mathematical approach, assuming that the reaction proceeds at the steady state, which permits calculation of a tentative substrate concentration to be used to determine low inhibitor concentrations and to obtain the value of inhibition constant corresponding to the inhibitor. The mathematical approach predicts a linear relationship between the inverse of the relative inhibition and the inverse of the inhibitor concentration. The method developed is applied to the acetylcholinesterase inhibitor galantamine, using an acetylcholine-selective electrode. A linear relationship for galantamine concentration from 2x10(-8) to 1 x 10(-6) M and a limit of detection of 5.4 x 10(-9) M was found. A value for K-l(Gal) of 2.0 x 10(-7)+/- 0.1 x 10(-7) M was obtained. The effect of several other drugs and of the main galantamine metabolite excreted in urine was studied. The method was satisfactorily applied to the determination of galantamine in pharmaceuticals and human urine. (C) 2013 Elsevier B.V. All rights reserved.
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