Journal
TALANTA
Volume 116, Issue -, Pages 508-513Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2013.07.032
Keywords
Fluorescent probe; Mercapto group detection; Maleimide; Protein detection
Categories
Funding
- Ministry of Science and Technology [2013CB933501]
- National Natural Science Foundation [91227202, 21074004]
- Ministry of Education of China [NCET-08-0005]
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Two water soluble fluorescent probes with quaternary ammonium salts as solubilization groups were designed and synthesized to detect proteins with close mercapto groups. The unique structure of V-shape oligo(o-phenylene-ethynylene) backbone connecting two maleimide groups as mercapto group recognition sites enables the probes to distinguish a single mercapto group from two close mercapto groups in proteins. The probe had no fluorescence response to proteins with only one mercapto group, but had strong fluorescence response to proteins with mercapto group combination at the concentration of 10(-6) mol/L. The probe worked under a wide range of pH, from 4 to 8. This work also demonstrates the concept of functional group combination detection. (C) 2013 Elsevier B.V. All rights reserved.
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