Article
Biology
Kirin D. Gada, Jordie M. Kamuene, Takeharu Kawano, Leigh D. Plant
Summary: The cell surfaceome plays a crucial role in biology, but the identification and regulation of proteins on the cell membrane remains challenging. TIRFM is a precise and effective technique that helps to locate proteins on the cell membrane by limiting image depth and improving signal-to-noise ratio.
Article
Biochemistry & Molecular Biology
Paras Gaur, Fletcher E. Bain, Masayoshi Honda, Sophie L. Granger, Maria Spies
Summary: As many as 700,000 unique sequences in the human genome can fold into G-quadruplexes (G4s), which play important roles in various cellular processes. A small protein called G4P has been synthesized and shown to bind G4 structures with better selectivity than previous methods. By studying its interaction with different G4s, the researchers found that increasing the number of recognition motifs in G4P enhances its affinity for certain G4s and its ability to interact with sequences containing multiple G4s.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Ling Li, Xin Shu, Litao Zhang, Ao Xu, Juan Yang, Yisha Jing, Hui Wang, Zhenlu Zhang
Summary: Cardiac troponin is a key biomarker for diagnosing acute coronary syndrome. This study evaluated the analytical and clinical diagnostic performance of a high-sensitivity cardiac troponin I (hs-cTnI) assay and compared it with another established assay. The results showed that Mindray's hs-cTnI (CLIA) assay is a precise and sensitive method for diagnosing myocardial infarction, with clinical diagnostic performance comparable to the established assay.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Joerg Nikolaus, Kasey Hancock, Maria Tsemperouli, David Baddeley, Erdem Karatekin
Summary: Researchers used a polarization-controlled total internal reflection fluorescence (pTIRF) microscope to monitor the fusion of proteoliposomes with planar lipid bilayers, investigating membrane fusion pore dynamics. By varying excitation polarization angle, they identified a dye-dependent optimal polarization for maximal fluorescence increase upon fusion, aiding event detection and lipid transfer kinetics analysis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Teliang Zhang, Xueliang Wang, Youjun Zeng, Songfeng Huang, Xiaoqi Dai, Weifu Kong, Qian Liu, Jiajie Chen, Junle Qu, Yonghong Shao
Summary: Surface plasmon resonance microscopy (SPRM) is widely used in biological fields for its high spatial resolution and label-free detection. In this study, a home-built SPRM system based on total internal reflection (TIR) was utilized to study the imaging of a single nanoparticle. By using a ring filter and deconvolution algorithm, the parabolic tail of the nanoparticle image was removed, achieving a spatial resolution of 248 nm. Additionally, specific binding between human IgG antigen and goat anti-human IgG antibody was successfully measured using TIR-based SPRM, demonstrating its capability in imaging sparse nanoparticles and monitoring biomolecular interactions.
Article
Chemistry, Physical
Jing Zeng, Wen Fan, Kangyu Jia, Shuhua Tu, Limin Wu
Summary: A novel retroreflective structural color film (RSCF) based on interference and total internal reflection (TIR) was reported. The film consists of a microscale polymer hemisphere array, displaying different colors under coaxial and noncoaxial illumination and observation. Additionally, an elastic polyurethane-based RSCF can exhibit dynamically changing colors during uniaxial tensile/compressive deformation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Ying Fu, Tunan Chen, Ligang Chen, Yuansen Guo, Zhongbo Yang, Ning Mu, Hua Feng, Mingkun Zhang, Huabin Wang
Summary: In this study, we fabricate a polydimethylsiloxane microfluidic chip suitable for measuring aqueous samples and investigate the effects of its configuration on terahertz spectra. The experimental results show that the Fresnel formulae of the two-interface model should be used when the chip depth is smaller than 210 μm, and the Fresnel formula of the one-interface model can be applied when the depth is no less than 210 μm. This conclusion is further validated by measuring physiological solution and protein solution. This work can promote the application of terahertz attenuated total reflection spectroscopy in studying aqueous biological samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Ernesta Buzavaite-Verteliene, Vincentas Maciulis, Justina Anulyte, Tomas Tolenis, Algirdas Baskys, Ieva Plikusiene, Zigmas Balevicius
Summary: In this study, a one-dimensional photonic crystal with an additional TiO2 layer, supporting Bloch surface waves (BSW), was used to enhance the signal sensitivity for protein interaction detection. The results showed that the BSW component of the photonic crystal sample with an additional TiO2 layer had higher sensitivity.
Article
Chemistry, Multidisciplinary
Nathaniel E. Sturniolo, Krista Hirsch, Caleb H. Meredith, Beau C. Beshires, Shawn Khanna, Malak S. Rayes, Michael A. Gallegos, Shannon McGee, Bryan Kaehr, Lauren D. Zarzar
Summary: This article presents an experimental investigation and optical modeling of structural coloration produced from total internal reflection interference within 3D microstructures. The iridescence generated for various microgeometries is modeled, examined, and rationalized using ray-tracing simulations, color visualization, and spectral analysis techniques. The observed iridescence is deconstructed into elementary components and linked to ray trajectories, which emanate from the illuminated microstructures. The findings provide a conceptual framework for understanding and tailoring the optical and iridescent properties of microstructured surfaces.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Chongqi Zhou, Yang Xu, Xiaonan Zhang, Zhangyan Li, Tian Guan, Yanhong Ji, Yanhong Ji
Summary: A modified weak measurement scheme, rotatory biased weak measurement, is proposed to significantly improve the sensitivity and resolution of the total internal reflection sensor. The method introduces an additional phase in the post-selected procedure and generates an extinction point in the spectrum distribution. The performance of the sensor is analyzed, and an optimal refractive index resolution of 4 x 10(-7) RIU is demonstrated.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Acoustics
Hongfei Zhu, Timothy F. Walsh, Bradley H. Jared, Fabio Semperlotti
Summary: This study discusses the design and application of a nonlocal elastic metasurface for achieving broadband passive vibration isolation in a complex structural assembly. The experimental results confirm the significant vibration isolation capabilities of the nonlocal metasurface, even under non-ideal incidence conditions, extending the validity of the results. Additionally, the study demonstrates that a planar metasurface can be morphed into a curved interface while maintaining full wave control capabilities.
JOURNAL OF SOUND AND VIBRATION
(2022)
Article
Optics
Shijie Sun, Yuanhua Che, Qidong Yu, Yuhang Xie, Tianhang Lian, Xueqing Sun, Xibin Wang, Daming Zhang
Summary: A broadband mode-independent thermo-optic switch based on the total-internal-reflection effect is proposed and experimentally demonstrated on a polymer waveguide platform. By optimizing the geometric parameters of the switch, a mode-independent TO switching function with a large bandwidth and extinction ratio can be realized for different modes. The designed structure can also be cascaded to form a 1 x N switch network for mode-division multiplexing (MDM) systems, improving network flexibility.
Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yazgan Tuna, Amer Al-Hiyasat, Jonathon Howard
Summary: This paper presents a protocol for combining IRM with a commercial TIRF microscope for simultaneous imaging of MAPs and dynamic microtubules. This setup improves existing TIRF setups by eliminating the need for microtubule labeling and additional optical components, allowing for high-speed observation of MAPs interacting with dynamic microtubules.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Polymer Science
J. Alejandro Rivera-Moran, Peter R. Lang
Summary: This paper investigates the influence of noise effects, particle size, and sampling time on total internal reflection microscopy (TIRM) measurement results. It finds that detector shot noise and prolonged sampling times may cause erroneous results in steep parts of the interaction potential, while background noise has negligible effects. Noise does not significantly affect dynamic data, but lengthy sampling times and small probe particle radii can cause issues. Additionally, the study observes that dynamic results may differ from standard hydrodynamic predictions due to partial slip.
Article
Biotechnology & Applied Microbiology
Tae Hwan Shin, Abdurazak Aman Ketebo, Da Yeon Lee, Seungah Lee, Seong Ho Kang, Shaherin Basith, Balachandran Manavalan, Do Hyeon Kwon, Sungsu Park, Gwang Lee
Summary: Overdose of MNPs@SiO2(RITC) induced lipid peroxidation and decreased membrane fluidity in HEK293 cells. The aspect ratio of cells decreased, traction force increased, and cell movement velocity decreased. Metabotranscriptomics network analysis revealed interplay among lipid peroxidation, focal adhesion, and cell movement.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Suresh Kumar Chakkarapani, Seungah Lee, Seong Ho Kang
Summary: An ultrasensitive capsaicin sensor based on endogenous single-molecule fluorophore enhancement and quenching interface on gold nanoislands (GNIs) was developed using correlative fluorescence fluctuation optical spectroscopy. This method showed excellent detection limits for capsaicin and dihydrocapsaicin, making it a promising tool for the study of label-free single-molecule interactions.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Huong Thi Thuy Nguyen, Seungah Lee, Junghwa Lee, Ji-Hyoung Ha, Seong Ho Kang
Summary: A novel enhanced multiple nanoarray BA sensor was developed using a competitive reaction in an evanescent field, showing improved detection sensitivity and a wide dynamic range for simultaneous detection of three BA molecules. The sensor was validated through high-performance liquid chromatography and enzyme-linked immunosorbent assay, demonstrating excellent enhancement of detection sensitivity through combining competitive reaction and simultaneous detection of BA-conjugated quantum dots on the multiple nanoarray sensor.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Suresh Kumar Chakkarapani, Tae Hwan Shin, Seungah Lee, Kyung-Soo Park, Gwang Lee, Seong Ho Kang
Summary: This study quantified the intracellular trafficking of silica-coated magnetic nanoparticles in different cells using dSTORM, revealing differences in internalization efficiency and the impact on cell nuclei. The findings highlight the importance of understanding cellular localization and uptake efficiency of nanoparticles for biomedical applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Seungah Lee, Junghwa Lee, Indra Batjikh, Hyunung Yu, Seong Ho Kang
Summary: In this study, a highly sensitive hypoxia sensor based on quantum dots and gold nanodiscs was developed for the detection of HIF-1alpha in tumor cells. The sensor showed high accuracy and precision and can independently predict tumor progression and early cancer onset.
Article
Chemistry, Analytical
Seungah Lee, Junghwa Lee, Seong Ho Kang
Summary: We have developed a highly sensitive fluorescence sandwich immunoassay for detecting small single molecules. Our platform utilizes gold nanoplate-based biotin antibodies and streptavidin-fluorophores instead of streptavidin-horseradish peroxidase in a microplate-based competitive ELISA. The detection limit of our assay is 5 zeptomolar (5 x 10(-21) M), which is 5.4 x 10(10) times more sensitive than the traditional turn-off format ELISA.
Article
Chemistry, Analytical
Yingying Cao, Seungah Lee, Kyungsoo Kim, Seong Ho Kang
Summary: In this study, a four-dimensional cuboid multiangle illumination-based light-sheet super-resolution (4D CMLS) imaging system was developed to overcome optical illusions in cells. The system integrated total internal reflection fluorescence and a differential interference contrast microscope, and used a rotatable cuboid prism to change the direction of light and select the optimal illumination angle. The 4D CMLS imaging method enables in-depth 4D observation of samples, making it suitable for single-molecule and subcellular organelle observations in single cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Junghwa Lee, Seungah Lee, Gwang Lee, Seong Ho Kang
Summary: A single-molecule fourplex nanoimmunosensor has been developed for accurate and simultaneous detection of TSH, T3, and T4. This nanoimmunosensor shows a 1015-fold higher detection sensitivity compared to the conventional enzyme-linked immunosorbent assay, making it suitable for early diagnosis and prognosis monitoring of various thyroid diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yingying Cao, Seungah Lee, Kyungsoo Kim, Jong-Young Kwak, Seong Ho Kang
Summary: Researchers have developed an integrated multifunctional light-sheet nanoscopy technique that allows for simultaneous six-dimensional tracking of anisotropic nanoparticles in single living cells. The technique utilizes wavelength-dependent scattering of light by gold nanorods to trigger signals and achieve real-time photo-switching. This technique has the potential to be used for real-time monitoring of intracellular transport in specific regions of single living cells, as well as for non-fluorescence super-resolution imaging and tracking.
Review
Chemistry, Analytical
Seungah Lee, Seong Ho Kang
Summary: Fluorescence can be enhanced or quenched depending on the distance between the metal nanoparticle surface and the fluorophore molecule. Metal-enhanced fluorescence (MEF) has promising potential in fluorescence-based biological sensing. MEF-based biosensor systems can be categorized into two platforms: two/three-dimensional scaffolds or colloidal suspensions. This review summarizes the applications of wavelength-dependent carbon dots (UV-VIS), noble metals (VIS), and upconversion nanoparticles (NIR to VIS), which contribute to the enhancement of fluorescence through resonance energy transfer modulation, and provides a perspective on this topic.
Review
Chemistry, Analytical
Yucheng Sun, Zengnan Wu, Yuting Shang, Seong Ho Kang, Jin-Ming Lin
Summary: This article provides a brief overview of the technical development, applications, challenges, and future directions in single-cell analysis using single-molecule detection-based super-resolution microscopy (SMDSRM), aiming to guide researchers in promoting the methodological development and exploring the potential of SMDSRM for single-cell analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Business
Hyewon Park, Won-Moo Hur, Seongho Kang
Summary: This study explored the effects of outcome-based control (OBCS) and behavior-based control (BBCS) on creative selling, and examined the mediation of work engagement in this relationship. The findings showed that both OBCS and BBCS were positively related to creative selling. Work engagement mediated the relationship between OBCS and creative selling but not between BBCS and creative selling. The joint effect of OBCS and BBCS on work engagement was found to be hierarchical, with high OBCS and low BBCS leading to higher work engagement.
JOURNAL OF RETAILING AND CONSUMER SERVICES
(2023)
Article
Chemistry, Analytical
Yucheng Sun, Seungah Lee, Seong Ho Kang
Summary: The study utilized a cubic spline algorithm-based depth-dependent fluorescence-free three-dimensional light-sheet super-resolution microscopy with dual-wavelength illumination sources to investigate the distance of Mito-ER contacts in various live cells. The method showed improved localization precision in lateral and axial directions compared to previously used algorithms.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
