Enhanced fluorescence sensing of melamine based on thioglycolic acid-capped CdS quantum dots

A sensitive and simple method for the determination of melamine (MA) was developed based on the fluorescence enhancement effect of MA for thioglycolic acid-capped (TGA-capped) CdS quantum dots (QDs). Under optimum conditions, a good linear relationship was obtained from 2.0 x 10(-9) to 5.0 x 10(-5) M. The detection limit was 1.0 x 10(-9) M. which was much lower than the safety limit (2.5 ppm in USA and the UK; 1 ppm for infant formula in China). The solution pH, the adding sequence of the buffer solution and MA and surface modifiers of CdS QDs greatly influenced the enhancement extent of MA for CdS QDs. The fluorescence enhancement was attributed to the surface passivation of the surface states of QDs by amine group of MA. The method was applied to detect MA in raw milk with satisfactory results. The proposed method manifested several advantages such as high sensitivity, short analysis time, low cost and ease of operation. (C) 2012 Elsevier B.V. All rights reserved.