Journal
TALANTA
Volume 92, Issue -, Pages 58-64Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.01.041
Keywords
L-Arginine determination; Conductometric biosensor; Arginase; Urease; Quality-control analysis
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Funding
- European Commission [PIRSES-GA-2008-230802]
- National Academy of Sciences of Ukraine
- NATO [CBP.NUKR.CLG 984221]
- Rhone-Alpes Region for MIRA
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A highly sensitive conductometric biosensor for L-arginine determination was developed by exploiting the unique biorecognition capacities of two enzymes of urea cycle - arginase (E.C. 3.5.3.1) and urease (E.C. 3.5.1.5). The enzymes were co-immobilized in a single bioselective membrane on the working sensor, while a lysine rich bovine serum albumin (BSA) membrane was immobilized on the reference sensor, allowing differential measurements. The optimum percentage ratio of arginase and urease within the bioselective membrane was determined when the biosensor sensitivity to L-arginine and urea was optimum. Analytical characteristics of the conductometric biosensor for L-arginine determination were compared for two types of enzyme immobilization (cross-linking with glutaraldehyde (GA) and entrapment in the polymeric membrane). The optimum features in terms of the sensitivity, the linear range, and the detection limit (4.2 mu S/mM, 0.01-4 mM, and 5.0 x 10(-7) M, respectively) were found for L-arginine biosensor based on enzyme cross-linking with GA. A quantitative determination of L-arginine in the real sample (a drinkable solution Arginine Veyron) gave a satisfactory result compared to the data provided by the producer (a relative error was 4.6%). The developed biosensor showed high operational and storage stability. (C) 2012 Elsevier B.V. All rights reserved.
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