Journal
TALANTA
Volume 99, Issue -, Pages 637-642Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2012.06.054
Keywords
Quantum dots-coated silica nanospheres (QDs/Si); Screen-printed gold electrodes (SPGE); Thrombin; Electrochemical aptasensor
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Funding
- NSFC, China Postdoctoral Science Foundation [21005090]
- Postdoctoral Fund Project of Central South University
- Hunan Provincial Natural Science Foundation for Distinguished Young Scholars [09JJ1002]
- Open Foundation of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University
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A novel electrochemical aptasensor involving quantum dots-coated silica nanospheres (QDs/Si) and the screen-printed gold electrodes (SPGE) was developed for the detection of thrombin. The screen-printed electrodes with several advantages, including low cost, versatility, miniaturization, and mechanical regeneration after each measurement cycle, were employed. On the other hand, the gold nanoparticles (AuNPs) were electrodeposited on the surface of SPGE to obtain AuNPs/SPGE. And this sandwich format (Apt/thrombin/Apt-QDs/Si) was fixed on the AuNPs/SPGE to fabricate the electrochemical aptasensor. The bound CdTe QDs were dissolved in an acid-dissolution step and were detected by electrochemical stripping analysis. The proposed aptasensor has excellent performance such as high sensitivity, good selectivity and analytical application in real samples. The combination of nanoparticles with the screen-printed electrode is favorable for amplifying electrochemical signals, and useful for large-scale fabrication of the electrochemical aptasensors, which would lay a potential foundation for the development of the electrochemical aptasensor. (C) 2012 Elsevier B.V. All rights reserved.
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