Article
Chemistry, Analytical
Ning Zhao, Jiaci Shi, Ming Li, Pengcheng Xu, Xuefeng Wang, Xinxin Li
Summary: We developed an electrochemical sensor based on three-dimensional graphene networks (3DGNs) for efficient detection of alkaline phosphatase (ALP) activity. The sensor showed a quick response, cost-effectiveness, and non-destructiveness, making it promising for monitoring living adherent osteoblast cell activity and quantifying ALP in other biological systems and clinical samples.
Article
Chemistry, Physical
Nuo Chen, Ying-Xia Du, Geng Zhang, Wang-Ting Lu, Fei-Fei Cao
Summary: The study synthesized amorphous Ni sulfoselenide on the surface of Ni(OH)2 nanoplate supported by nickel foam, demonstrating high activity and durability for hydrogen evolution reaction and urea oxidation reaction in alkaline media.
Review
Chemistry, Analytical
Changdong Chen, Ming La, Xinyao Yi, Mengjie Huang, Ning Xia, Yanbiao Zhou
Summary: Electrochemical immunosensors with alkaline phosphatase (ALP) as the signal reporter have shown great potential in various fields. This work summarizes the achievements of these immunosensors, focusing on detection principles, signal amplification strategies, and challenges for improving the performance of ALP-based immunoassays.
Article
Biochemistry & Molecular Biology
Xiaofei Li, Jing Lu, Zutian Li, Haoyuan Yang, Weiming Li, Yanju Liu, Mingsan Miao
Summary: An electrochemical method for detecting ALP activity through ARGET ATRP was developed in this study, showing good analytical performance and promising applications.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Xin Zhu, Wenbin Wang, Jing Lu, Lulu Hao, Huaixia Yang, Yanju Liu, Fuchun Si, Jinming Kong
Summary: A novel electrochemical sensor based on signal amplification strategy through ring-opening polymerization (ROP) has been developed for the assay of alkaline phosphatase (ALP) activity. The sensor showed satisfactory linear dependence, low detection limit, and good selectivity in human serum samples, with a relative error of less than 5% compared to clinical data.
ANALYTICA CHIMICA ACTA
(2021)
Article
Electrochemistry
P. K. Neena, K. Reshma, Aarathi Pradeep, Bipin G. Nair, T. G. Satheesh Babu, Punathil Vasu Suneesh
Summary: This paper presents a novel electrochemical biosensor for estimating haemoglobin levels in alkaline conditions. The sensor shows high selectivity, linear response range, and sensitivity. It is validated by testing with whole blood samples.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Zhen-Zhen Mi, Hao-Cheng Hu, Jian-Jun Sun, Shao-Hua Wu
Summary: An ultrasensitive electrochemical biosensor for detecting p53 gene was fabricated based on heated gold disk electrode coupling with Nt.BstNBI-assisted target recycle amplification and ALP-based electrocatalytic signal amplification. The importance of this study is rated 8 out of 10.
ANALYTICA CHIMICA ACTA
(2023)
Article
Electrochemistry
Sina S. Jamali, Douglas Mills
Summary: Electrochemical noise measurement is commonly used for assessing organic coatings, and a stable reference electrode is essential for collecting valid data. The area of the electrode affects noise characteristics, with smaller electrodes generally producing less current noise but also having higher impedance, requiring optimization.
ELECTROCHIMICA ACTA
(2021)
Review
Materials Science, Multidisciplinary
Sonali Verma, Sandeep Arya, Vinay Gupta, Sarika Mahajan, Hidemitsu Furukawa, Ajit Khosla
Summary: The increasing environmental problems and energy demands have stimulated research into safe and efficient energy sources. Supercapacitors have attracted great interest due to their high energy storage capacity.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Biochemical Research Methods
Yaping Zhang, Peipei Li, Jing Lu, Daoxiang Li, Huaixia Yang, Xiaofei Li, Yanju Liu
Summary: In this study, a novel electrochemical sensor was developed for sensitive determination of alkaline phosphatase (ALP) activity using amifostine as the substrate and activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) as a signal amplification strategy. The proposed sensor showed high efficiency, simplicity of operation, and cost-effectiveness, and demonstrated potential application for ALP activity detection in human serum samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Energy & Fuels
Mehmet Erman Mert
Summary: The study investigated the efficiency of hydrogen production by doping nickel foam precursors with nickel, cobalt particles, and decorating with silver nano-domes. The catalyst NF/NiCo-2/Ag showed the best performance in terms of lower hydrogen overpotential, higher HPE, stability, and response during the electrolysis process in 1 M KOH.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
Article
Chemistry, Analytical
Cuiling Shang, Yansong Li, Qi Zhang, Song Tang, Xiaoqian Tang, Honglin Ren, Pan Hu, Shiying Lu, Peiwu Li, Yu Zhou
Summary: A dual-signal immunoassay utilizing alkaline phosphatase was developed to detect Zearalenone (ZEN) in cornmeal. The method combines colorimetric and electrochemical detection, generating Prussian blue nanoparticles and multicolor changes. The results demonstrate a high sensitivity for ZEN detection in corn samples through UV-vis spectrometry and differential pulse voltammetry.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Wenbin Wang, Jing Lu, Lulu Hao, Huaixia Yang, Xuejie Song, Fuchun Si
Summary: A novel electrochemical method utilizing aminoferrocene as an electroactive probe for alkaline phosphatase (ALP) activity detection was described in this study. The approach demonstrated good linear relationship and sensitivity in detecting ALP activity, with high selectivity and applicability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fuchun Si, Yaping Zhang, Jing Lu, Mengyuan Hou, Huaixia Yang, Yanju Liu
Summary: Alkaline phosphatase (ALP) is an important enzyme with significant implications in biomedical research and clinical diagnosis. A novel electrochemical biosensor based on photoinduced atom transfer radical polymerisation (photoATRP) has been developed for the analysis of ALP. This biosensor demonstrates a wide linear range, low detection limits, and excellent selectivity and interference resistance in human serum samples.
Article
Chemistry, Physical
Jacob Matthew Shellhamer, Paul Alexis Chando, Shreyas Pathreeker, Xinlu Wang, Ian Dean Hosein
Summary: With the increasing interest in postlithium technologies like calcium batteries, the scarcity of suitable reference electrodes poses a significant challenge. This study introduces a silver-(I) sulfide reference electrode for calcium-ion battery research, showing promise based on comprehensive structural characterization and electrochemical analysis. The standard reduction potential of Ag2S relative to the standard hydrogen electrode and the voltage drift over time have been determined. Ag2S is a potential reference electrode material for future calcium-ion battery research.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Review
Chemistry, Analytical
Siti Masturah Binti Fakhruddin, Kosuke Ino, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku
Summary: Bipolar electrode-based electrochromic devices have gained attention for analytical applications such as health monitoring, diagnostics, and chemical sensing. The review summarizes available electrochromic materials, discusses different device types, and reviews biological and chemical analytical demonstrations. The future developments of BPE-based electrochromic devices in analytical applications are then highlighted.
Article
Engineering, Biomedical
Yuji Nashimoto, Minori Abe, Ryota Fujii, Noriko Taira, Hiroki Ida, Yasufumi Takahashi, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku
Summary: This study evaluates the role of scanning probe microscopy (SPM) as an analytical tool for microphysiological systems (MPS), providing a new solution for real-time high spatial resolution imaging. A simple vascular model was used for permeability and topographical analysis, paving the way for the application of SPM technology in MPS.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Analytical
Rise Akasaka, Masashi Ozawa, Yuji Nashimoto, Kosuke Ino, Hitoshi Shiku
Summary: A novel methodology based on ion conductance was proposed to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. By analyzing the ion current changes, the perfusability of vessels could be determined, serving as an important tool for drug screening using organs-on-a-chip.
Article
Chemistry, Multidisciplinary
Kosuke Ino, Hao-Jen Pai, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku
Summary: A novel electrochemical imaging method was introduced to evaluate endothelial permeability by monitoring the diffusion of tracers through a monolayer of HUVECs. The method demonstrated its potential in studying the effects of histamine on endothelial monolayers and evaluating the metastasis of cancer spheroids. The developed device provides spatial information and allows for monitoring the interaction between monolayers and spheroids, showing promise for organs-on-a-chip and in vitro drug screening applications.
Article
Electrochemistry
Kosuke Ino, Keika Komatsu, Kaoru Hiramoto, Yoshinobu Utagawa, Yuji Nashimoto, Hitoshi Shiku
Summary: This study presents a method for electrochemiluminescence (ECL) imaging to visualize cellular adhesion on hydrogel scaffolds. By culturing human umbilical vein endothelial cells on a Matrigel-coated indium tin oxide (ITO) electrode and using an ECL solution and specific voltage, ECL signals were generated, allowing for imaging of cellular adhesion.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku
Summary: A novel electrochemical strategy for gluing hydrogel fibers is presented in this study. The method involves using microelectrodes to generate chemicals for crosslinking the fibers. The results show that the electrochemical glue is non-toxic to cells, making it a promising method for tissue engineering and organ-on-a-chip development.
Editorial Material
Chemistry, Analytical
Kosuke Ino
Article
Chemistry, Analytical
Kaoru Hiramoto, Kazuyuki Iwase, Yoshinobu Utagawa, Yuji Nashimoto, Itaru Honma, Kosuke Ino, Hitoshi Shiku
Summary: Endothelial cells are commonly used for vascular biology studies. Recent advancements in tissue engineering have enabled the development of three-dimensional culture systems for vascular endothelial cells, which can serve as physiologically relevant models. To facilitate research, an electrochemical device has been developed for detecting nitric oxide (NO), a crucial molecule in the vasculature, to evaluate endothelial cells cultured in a 3D environment.
ANALYTICAL SCIENCES
(2022)
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku
Summary: A highly sensitive immunoassay for human immunoglobulin G (IgG) using signal amplification of the coagulation cascade is reported. The reaction product, pNA, was quantified using square wave voltammetry with a glassy carbon electrode. Thrombin efficiently reacted with the substrate FPK-pNA and factor XIa was not inactivated during labeling. The coagulation cascade-based immunoassay showed high selectivity and sensitivity for human IgG.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku
Summary: In this study, a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode was developed. The dual immunoassay showed high sensitivity for detecting two types of analytes, and the signal amplification by the cascade reactions and the use of redox cycling in a nanoscale gap contributed to the high sensitivity. The cross reaction between the LAL and coagulation cascade reactions was minimized by bisecting the beads after the immunocomplex formation. The dual immunoassay demonstrated its potential for clinical diagnosis requiring the detection of multiple analytes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku
Summary: In this study, we developed an electrochemical endotoxin sensor using an interdigitated array electrode (IDAE) device. The IDAE device showed high redox cycling efficiency and signal amplification rate. The sensor successfully detected p-aminophenol (pAP) released from Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as a product of an endotoxin-induced LAL reaction. The sensor could detect as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these values were within the cut-off range for ultrapure dialysis fluid. Therefore, the highly sensitive endotoxin sensor is useful for ensuring medical safety.
Article
Biochemistry & Molecular Biology
Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Hitoshi Shiku
Summary: Hydrogels are widely used in cell culture applications, and specific structures are necessary for fabricating tissues and organs. Existing techniques for producing multiple-branched hydrogels are inefficient and time-consuming. To address this issue, a simple and rapid fabrication method based on viscous fingering is proposed, which only requires two plates. High-viscosity solution is introduced into the space between the plates and one plate is peeled off, resulting in the formation of multi-branched structures. This approach allows for the successful fabrication of multi-branched hydrogels within 1 minute.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Chemistry, Physical
An Konno, Kosuke Ino, Yoshinobu Utagawa, Hitoshi Shiku
Summary: Microphysiological systems (MPSs) are cell culture systems that use mammalian cells to construct organs and tissues in vitro. Electrochemical sensors have become popular in MPSs due to their simplicity and sensitivity. This review summarizes recent advances in electrochemical imaging of MPSs and provides insightful perspectives.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Engineering, Biomedical
Yoshinobu Utagawa, Kosuke Ino, Masahiro Takinoue, Hitoshi Shiku
Summary: This study presents a simple and rapid method for fabricating core-shell hydrogel fibers for cell culture applications. The core fibers are prepared using interfacial polyelectrolyte complexation with chitosan and DNA, and then coated with calcium alginate shell hydrogels. This method has wide applications in tissue engineering and cell analysis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Analytical
Yoshinobu Utagawa, Kosuke Ino, Kaoru Hiramoto, Kazuyuki Iwase, Yuji Nashimoto, Itaru Honma, Hitoshi Shiku
Summary: In this study, a novel microfluidic device with a porous membrane electrode was developed for the in situ monitoring of nitric oxide (NO) released by endothelial cells in real time. This device successfully measured NO released by human umbilical vein endothelial cells and recorded the changes in NO release with culture time. This research contributes to the development of organ-on-a-chip technology for real-time in situ cell analyses.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
