Review
Chemistry, Analytical
Muhammad Sajid
Summary: Since 2006, dispersive liquid-liquid microextraction (DLLME) has undergone several improvements in various aspects, including extraction device design, extractant dispersion strategies, solvent selection, and phase separation approaches. It has also been combined with other extraction techniques for wider applications. This review critically evaluates the advancements in simultaneous derivatization, automation, and instrumental coupling.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Kawin Khachornsakkul, Ruben Del-Rio-Ruiz, Lita Chheang, Sameer Sonkusale
Summary: This study reports the use of distance-based thread analytical devices (dTADs) combined with dispersive liquid-liquid microextraction (DLLME) method for monitoring trace melatonin levels in human samples and dietary supplements. The proposed method is simple, inexpensive, sensitive, and exhibits remarkable reproducibility and selectivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ahmed Mostafa, Heba Shaaban, Abdulmalik M. Alqarni, Meshal Alghamdi, Semat Alsultan, Jenan Saleh Al-Saeed, Sahar Alsaba, Amal AlMoslem, Yasir Alshehry, Rizwan Ahmad
Summary: This study explores the use of natural deep eutectic solvents (NDESs) for the extraction and trace analysis of sulfonamides. The results show that using a thymol/acetic acid mixture as the extracting solvent yields the best extraction recovery. The proposed method is found to be superior to other methods based on ecological assessments and other metrics. Overall, this method can serve as a valid eco-friendly alternative for the routine analysis of sulfonamides in different water samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Marina Pereira-Coelho, Isabel Cristina da Silva Haas, Cassia Katrin Reinke, Jocinei Dognini, Renata Dias de Mello Castanho Amboni, Luciano Vitali, Luiz Augusto dos Santos Madureira
Summary: A simple sample preparation approach employing the dispersive pipette extraction (DPX) technique is proposed to determine twelve polyphenols in wines. The extraction parameters were optimized using univariate and multivariate designs. The analytical performance was satisfactory with high determination coefficients and good precision.
Article
Chemistry, Applied
Malek Hassan, Furkan Uzcan, Usama Alshana, Mustafa Soylak
Summary: A novel method combining switchable-hydrophilicity liquid-liquid microextraction and magnetic nanoparticle-based dispersive solid-phase microextraction was proposed for the determination of erythrosine using UV/Vis spectrophotometry. The method showed excellent analytical performance with high sensitivity, low detection limits, and good reproducibility, making it suitable for the extraction and determination of erythrosine in food and consumer products.
Article
Chemistry, Analytical
Valfredo Azevedo Lemos, Jeferson Alves Barreto, Luana Bastos Santos, Rosivan dos Santos de Assis, Cleber Galvao Novaes, Ricardo J. Cassella
Summary: Dispersive liquid-liquid microextraction (DLLME) is widely used for the separation and preconcentration of various chemical species, with some systems using a syringe as an extraction environment. This review discusses methods utilizing this approach, critical aspects like dispersion promotion, analytical characteristics, and the pros and cons of the methods, as well as future trends in in-syringe microextraction systems.
Article
Chemistry, Multidisciplinary
Qian Liu, Zhiqiang Li, Liulin Wei, Xiaomei Chen, Yidong Xu, Jingchan Zhao
Summary: In this study, a thermo-switchable deep eutectic solvent was used to improve the extraction of organophosphorus pesticides and pyrethroids in water samples. The method showed fast extraction, high precision, and low detection limit.
ENVIRONMENTAL CHEMISTRY LETTERS
(2022)
Article
Biochemical Research Methods
Laura Carbonell-Rozas, Romina Canales, Francisco J. Lara, Ana M. Garcia-Campana, Maria Fernanda Silva
Summary: By utilizing natural deep eutectic solvents (NADES) as extracting solvents, combined with dispersive liquid-liquid microextraction (DLLME-SFO) method, pesticide residues in environmental water and white wine samples were successfully extracted and preconcentrated, achieving high recoveries and low detection limits. This method demonstrates a new application for NADES in sample preparation and highlights its greenness compared to other reported approaches.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Giulia Brick Grecco, Kathleen Fioramonte Albini, Luiz Sidney Longo Jr, Marcio Adriano Andreo, Bruno Lemos Batista, Felipe Rebello Lourenco, Leandro Augusto Calixto
Summary: Arnicas Solidago microglossa DC. and Arnica montana L. are widely used natural medicines in Brazil, but they can easily be contaminated by harmful elemental impurities. This study optimized the use of dispersive liquid-liquid microextraction (DLLME) for the determination of metallic impurities in arnica infusions. By replacing toxic solvents with green chemistry solvents, the DLLME method proved to be an effective and more accessible alternative to expensive techniques like ICP.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Julia Martinelli Magalhaes Kahl, Kelly Francisco da Cunha, Leonardo Costalonga Rodrigues, Kaue de Oliveira Chinaglia, Karina Diniz Oliveira, Jose Luiz Costa
Summary: The study developed an analytical method using DLLME combined with LC-MS/MS to quantify amphetamine and derivatives in oral fluid samples. The method reduces solvent usage in samples preparation, lowers the amount of solvents and reagents used in analytical-instrumental stage, and requires a minimal sample volume.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Malek Hassan, Furkan Uzcan, Syed Nasrullah Shah, Usama Alshana, Mustafa Soylak
Summary: The combination of switchable-hydrophilicity solvent-liquid-liquid microextraction (SHS-LLME) and dispersive solid-phase microextraction (DSPME) showed efficient preconcentration and determination of quercetin, with high recovery rates and good repeatability. The proposed method is superior in terms of greenness, rapidness, simplicity, and low capital cost, making it a promising technique for sample cleanup and analysis.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Environmental Sciences
A. Bayati-Comitaki, M. M. Zahedi
Summary: This research introduces an easy, inexpensive, and fast spectrophotometric method for measuring boron in water samples. By optimizing the reaction conditions and using a flow-based analysis, the study achieved accurate measurement of boron content.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Review
Chemistry, Analytical
Jose Grau, Cristian Azorin, Juan L. Benede, Alberto Chisvert, Amparo Salvador
Summary: Dispersive liquid-liquid microextraction is a widely used technique in analytical chemistry field due to its simplicity and rapidity. The operational mode has evolved over time, with changes mainly related to the nature of solvents used. Low-density solvents, ionic liquids, and other alternatives have been proposed as greener options for this method.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Multidisciplinary Sciences
Weidong Li, Jianping Qiu, Leila Baharinikoo, T. Ch Anil Kumar, Basim Al-qargholi, Shafik S. Shafik, Reathab Abbass, Shelesh Krishna Saraswat
Summary: In this study, a low-cost, fast, and easy-to-use ultrasound-assisted dispersive-magnetic nanocomposites-solid-phase microextraction (UA-DMNSPME) method was developed for the preconcentration and determination of trace amounts of Auramine O (AO) and methylene blue (MB) dyes in environmental water samples. The method utilized magnesium oxide nanoparticles (MgO-NPs) as adsorbents and showed high preconcentration factor, low limits of detection, and short extraction time. The proposed method can be considered efficient for the extraction and preconcentration of AO and MB from water and wastewater samples.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Mehrnoosh Gholami, Mohammad Faraji, Shahryar Jafarinejad
Summary: A hydrophobic deep eutectic solvent (DES) was synthesized for dispersive liquid-liquid microextraction (DLLME) of Pb(II) in water samples, followed by determination using UV-Vis spectrophotometry. The DES was prepared by mixing thymol and benzyltriethylammonium chloride as the hydrogen bond donor and acceptor, respectively. The method exhibited excellent sensitivity and precision, with a linear calibration curve in the concentration range of 1.0-200 ng mL(-1) and a recovery rate above 90% in real water samples.
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
(2023)
Review
Chemistry, Analytical
Carlos Bendicho, Isela Lavilla, Francisco Pena-Pereira, Inmaculada de la Calle, Vanesa Romero
Summary: The development of disposable sensors adaptable to various analytical problems is a hot topic in science and technology. Despite limitations, such sensors offer advantages such as low cost, portability, low sample consumption, and fast response. The advancement of paper-based analytical devices has made on-site detection of toxic metal ions and pollutants more accessible.
Article
Biochemical Research Methods
Marta Aranaz, Marta Costas-Rodriguez, Lara Lobo, Montserrat Garcia, Hector Gonzalez-Iglesias, Rosario Pereiro, Frank Vanhaecke
Summary: The study aimed to investigate local alterations in glaucomatous patients, focusing on total antioxidant capacity and mineral element concentrations. Results showed higher total antioxidant capacity in glaucoma patients, as well as significant differences in magnesium and phosphorus levels compared to the control group.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Francisco Pena-Pereira, Vanesa Romero, Inmaculada de la Calle, Isela Lavilla, Carlos Bendicho
Summary: Graphene and graphene oxide have been extensively studied as sorbents for various applications, with efforts focused on enhancing their properties through functionalization, magnetization, and synthesis of novel nanocomposites. The latest innovations in utilizing graphene and GO platforms for sorption, including introducing new functionalities and hybridizing with other nanomaterials, are reviewed in this article, showcasing their preparation methods, analytical characteristics, applications, and future prospects.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Rosa Grigoryan, Marta Costas-Rodriguez, Elien Van Wonterghem, Roosmarijn E. Vandenbroucke, Frank Vanhaecke
Summary: Injection of LPS in mice induces endotoxemia, leading to (neuro)inflammation and sepsis. The isotopic composition of Mg in blood plasma and liver was significantly affected, with aged mice showing additional impact on urine Mg isotopic composition. Additionally, a regional endotoxin-induced difference in brain Mg isotopic composition was observed for aged mice only.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Francisco Pena-Pereira, Isela Lavilla, Carlos Bendicho
Summary: This review examines cutting-edge advances in greener analytical methods for sample preparation, focusing on the use of environmentally friendly solvents and adsorbents, integration of unitary steps, miniaturization, and automation. The paper also addresses concluding remarks and additional considerations for further improvements.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lorena Placer, Laura Estevez, Isela Lavilla, Francisco Pena-Pereira, Carlos Bendicho
Summary: This study evaluated luminescent probes derived from citric acid and β-aminothiols for fluorescent sensing purposes, showing promising pH-dependent dual excitation/dual emission features. The research also demonstrated successful implementation of paper-based devices modified with CA-Cys for sensitive and selective ammonia sensing, with a remarkable enrichment factor and limit of detection under optimal conditions.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Vanesa Romero, Isela Lavilla, Alicia Alvarez, Carlos Bendicho, Begona Espina, Laura M. Salonen
Summary: In this study, a new analytical approach based on ultrasound-assisted dispersive (micro)solid phase extraction (US-D-uSPE) using TpBD-Me-2 covalent organic framework (COF) as the adsorbent was proposed for the fluorescent determination of two polycyclic synthetic fragrances in seawater. The method demonstrated high adsorption efficiencies and desorption efficiencies, as well as low limits of detection for the target analytes. It was successfully applied to seawater analysis without the need for a previous sample treatment, showing great potential for environmental monitoring of emerging organic pollutants.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Carlos Bendicho, Isela Lavilla, Francisco Pena-Pereira, Inmaculada de la Calle, Vanesa Romero
Summary: Lab-on-paper technologies, also known as paper-based analytical devices (PADs), have gained increasing attention in recent years and are now widely used in various fields such as medical diagnostics, food safety, and environmental monitoring. The focus on heavy metals as important environmental pollutants, along with the advantages of on-field detection, are driving developments in this area. Detection methods using recognition elements like organic chromophores/fluorophores, plasmonic nanoparticles, and other materials are being explored for their selectivity and sensitivity in monitoring toxic metals like Hg in the environment.
Article
Chemistry, Analytical
Lorena Placer, Isela Lavilla, Francisco Pena-Pereira, Carlos Bendicho
Summary: This study presents a sensitive and cost-effective non-instrumental method for determining bromine species in environmental waters by combining paper-based headspace sampling and smartphone-based fluorescence detection. The method utilizes a luminescent probe derived from natural compounds to achieve sensitive detection of bromine species under non-instrumental conditions with high stability and accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Nerea Villarino, Francisco Pena-Pereira, Isela Lavilla, Carlos Bendicho
Summary: This study assessed the use of widely available waterproof cellulose-based substrates for sensitive in-drop plasmonic sensing approaches. The results showed that waterproof cellulose-based substrates are convenient platforms for analytical purposes and can be used as sample compartments for smartphone-based colorimetric sensing. The stability and applicability of spherical, rod-shaped, and core-shell metallic nanoparticles on waterproof cellulose-based substrates were also evaluated, with Au@AgNPs showing potential for colorimetric detection of in situ generated H2S, I-2, and Br-2.
Article
Chemistry, Analytical
Vanesa Romero, Carolina Sant'Anna, Isela Lavilla, Carlos Bendicho
Summary: A novel analytical approach combining photocatalytic decomposition and headspace thin-film microextraction was developed for the determination of acyclic N-nitrosamines in drinking water. A fluorescent cellulose substrate modified with silver nanoclusters was used as both extractant and sensing platform, and the fluorescence quenching was measured using front-face fluorescence spectroscopy. The method showed good sensitivity and accuracy for the detection of acyclic N-nitrosamines.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Isela Lavilla, Vanesa Romero, Paula Costas, Carlos Bendicho
Summary: A new analytical approach combining ultrasound-assisted emulsification, photoreaction with methylene blue, and UV-vis spectrophotometry has been developed to determine l-ascorbic acid (AA) in cosmetic samples. The emulsification process produces a transparent solution that is easily measured by UV-vis spectrophotometry. The reaction between the emulsified sample and methylene blue is irradiated with a tungsten lamp for 5 minutes, resulting in a decrease in absorbance intensity as the AA concentration increases. The proposed method has high sensitivity, with limits of detection and quantification of 0.04 μg mL(-1) and 0.15 μg mL(-1), respectively.
ANALYTICAL METHODS
(2023)
Editorial Material
Chemistry, Analytical
Carlos Bendicho
MICROCHEMICAL JOURNAL
(2023)
Article
Anthropology
IJk van Hattum, Marta Costas-Rodriguez, Kasper Hobin, Frank Vanhaecke, Hans Vandendriessche, Helene Collet, Pierre Cattelain, Michel Toussaint, Quentin Goffette, Maarten Dhaenens, Jessica L. A. Palmer, Simon Daled, Philippe Crombe, Isabelle De Groote
Summary: This study examines the mobility of individuals in the Neolithic period in Belgium and finds evidence of four nonlocal individuals based on the strontium isotope ratios in their dental enamel. The results support the hypothesis of connections with Northern France.
AMERICAN JOURNAL OF BIOLOGICAL ANTHROPOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Kasper Hobin, Marta Costas-Rodriguez, Elien Van Wonterghem, Roosmarijn E. Vandenbroucke, Frank Vanhaecke
Summary: The concentration and isotopic composition of Cu and Fe in blood plasma and specific brain regions were investigated to assess potential alterations associated with sepsis-associated encephalopathy. Significant variations were observed in Cu concentration and isotopic ratios in the blood plasma. Age and inflammation affected the isotopic compositions, while Cu isotope redistribution was found in the brain tissues.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).