Journal
TALANTA
Volume 85, Issue 4, Pages 1933-1940Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2011.07.018
Keywords
In vivo microdialysis; Liquid chromatography; Dopamine; Glutamate; Brain bioenergetics; Glucose; Lactate; Pyruvate; Ascorbic acid; Rat striatum
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Funding
- Regione Sardegna Legge Regionale 7 agosto
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Microdialysis is an extensively used technique for both in vivo and in vitro experiments, applicable to animal and human studies. In neurosciences, the in vivo microdialysis is usually performed to follow changes in the extracellular levels of substances and to monitor neurotransmitters release in the brain of freely moving animals. Catecholamines, such as dopamine and their related compounds, are involved in the neurochemistry and in the physiology of mental diseases and neurological disorders. It is generally supposed that the brain's energy requirement is supplied by glucose oxidation. More recently, lactate was proposed to be the metabolic substrate used by neurons during synaptic activity. In our study, an innovative microdialysis approach for simultaneous monitoring of catecholamines, indolamines, glutamate and energy substrates in the striatum of freely moving rats, using an asymmetric perfusion flow rate on microdialysis probe, is described. As a result of this asymmetric perfusion, two samples are available from the same brain region, having the same analytes composition but different concentrations. The asymmetric flow perfusion could be a useful tool in neurosciences studies related to brain's energy requirement, such as toxin-induced models of Parkinson's disease. (C) 2011 Elsevier B.V. All rights reserved.
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