Article
Nanoscience & Nanotechnology
Wenting Kong, Feiran Li, Yunlu Pan, Xuezeng Zhao
Summary: A hygro-responsive superoleophobic/superhydrophilic coating was successfully fabricated, showing superior selective superwettability under different conditions and efficient separation of oil-water mixtures with a separation efficiency of over 99%. Additionally, the long-chain perfluoroalkyl substances on the coating could be decomposed by UV irradiation, reducing harm to the environment and human beings.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Polymer Science
Guangran Shao, Yuan Liu, Rong Cao, Guang Han, Bing Yuan, Wangqing Zhang
Summary: Thermo-responsive block copolymers have attracted a lot of attention due to their mature synthesis technology, well-defined structure, controllable molecular weight, and easy implementation of their nano-assemblies in water through temperature regulation. Previous reviews have mainly focused on their biomedical applications, but these polymers also show promising potential in temperature-regulated adsorption and/or separation, nanoreactors for green organic synthesis, etc. This review summarizes the recent progress in thermo-responsive assembly in water and highlights the applications of five different thermo-responsive block copolymers beyond the biomedical field.
Article
Chemistry, Multidisciplinary
Honglei Wang, Jun Shi, Jingyu Zhang, Zhehao Tao, Hongguang Wang, Qingqing Yang, Peter A. van Aken, Runfeng Chen
Summary: This study presents a facile and green method for preparing few-layer MoS2 nanosheets and nanocomposites, and demonstrates their potential in high-performance memory devices. The findings reveal the charge trapping and detrapping behaviors of the 2D MoS2-based nanocomposites.
Article
Chemistry, Physical
Zhixia Wang, Hang Song
Summary: Compared with traditional microemulsion, stimulating stimulus-responsive microemulsion is more challenging to achieve. A temperature-responsive microemulsion was successfully formulated and optimized by using the mixture of tropine-based ionic liquids (TILs) and water as a polar phase, different aliphatic oils as a nonpolar phase, and a mixture of nonionic surfactant Triton X-100 (TX-100) and short-chain aliphatic alcohols as a cosurfactant. The results showed that cyclohexane had better compatibility with TX-100/1-octanol to form the TILs-based microemulsion, and the monophasic region increased with the alkyl chain length of TILs cation group. The temperature-responsive microemulsion exhibited sensitivity to temperature, which decreased with the increase of TILs-to-water mass ratio and TILs side chain length.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Marziyeh Kavian, Milad Ghani, Jahan Bakhsh Raoof
Summary: An affordable high-throughput microextraction strategy using poly-oxometalate/reduced graphene oxide (POM/rGO) composite was developed for the extraction of parabens from real samples. The method showed high extraction efficiency due to the uniform stabilization of POMs on rGO and the hydrophobic interactions between rGO and the target analytes. The method was validated and applied successfully for the analysis of parabens in cosmetic products without interference.
MICROCHEMICAL JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Robbert J. de Haas, Ketan A. Ganar, Siddharth Deshpande, Renko de Vries
Summary: Biomolecular condensates are macromolecular complexes formed by liquid-liquid phase separation that regulate key biological functions. Designing stimuli-responsive synthetic condensates is crucial for engineering compartmentalized synthetic cells that can mimic spatiotemporal control over biochemical reactions.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Mathijs Baert, Kristina Wicht, Ali Moussa, Gert Desmet, Ken Broeckhoven, Frederic Lynen
Summary: TRLC utilizes temperature responsive polymers to control retention, eliminating the need for solvent gradients and allowing more environmentally friendly separations. The study shows that temperature gradients can control retention and enhance refocusing of analyte peaks.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Physical
Mitsuhiro Honda, Tsuyoshi Ochiai, Popy Listiani, Yuma Yamaguchi, Yo Ichikawa
Summary: This study demonstrates the preparation of Cu-doped TiO2 structures through liquid phase deposition at a low temperature, which harnesses the energy from sunlight to solve green- and energy-related problems.
Article
Chemistry, Analytical
Adriaan Ampe, Elena Bandini, Ken Broeckhoven, Frederic Lynen
Summary: Temperature-responsive liquid chromatography (TRLC) offers an alternative approach for optimization of retention and selectivity in HPLC. By utilizing temperature gradients on stimuli-responsive stationary phases and eliminating the need for solvent gradients, TRLC can be used with aqueous mobile phases, making it a green alternative to reversed-phase separations. This study developed a flow-through modification procedure that allows on-column modification of aminopropyl silica columns, achieving identical column efficiency compared to existing TRLC columns. Flow-through produced columns showed higher retention at elevated temperatures despite reduced carbon load. The presence of stationary phase gradients was observed, and its influence on retention was studied through novel retention experiments.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Paniz Tashakkori, Aylin Altinisik Tagac, Melek Merdivan
Summary: Novel montmorillonite composites with ionic liquid coatings were prepared for solid phase microextraction fibers, and SPME methods for phenolic compounds determination were developed. The results showed satisfactory relative recoveries and repeatability in fruit juice samples, and good agreement between the two developed chromatographic methods was observed.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Materials Science, Multidisciplinary
Hao Xue, Fei Liu, Ze Wang, Delei Liu, Liang Zhou, Wenbo Su, Shichao Niu, Zhiwu Han, Luquan Ren
Summary: This article presents a simple and rapid in situ polymerization method for preparing dual-responsive photonic crystals (PCs) with ultra-precise micro-/nanoscale hierarchical structures. The obtained P(NIPAAM-co-AAc)-PCs exhibited a dual response to pH and temperature reversibility. This work allows the combination of natural hierarchical PC structures with smart hydrogel to achieve an optical response to pH and temperature, thus opening up the channels for responsive PC to multiple stimulus responses.
MATERIALS & DESIGN
(2023)
Article
Chemistry, Analytical
Elena Bandini, Kristina Wicht, Adriaan Ampe, Mathijs Baert, Hamed Eghbali, Frederic Lynen
Summary: Refractive index detection is an attractive method for liquid chromatography, but its implementation is limited due to its poor detection sensitivity and compatibility with only isocratic mobile phases. In this study, the combination of thermal gradient temperature-responsive liquid chromatography with refractive index detection was tested and proved to have minor baseline drift and easily controllable. Short chain fatty acids were used to assess this new approach.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Israel D. Souza, Igor G. C. Oliveira, Maria Eugenia C. Queiroz
Summary: Fiber-in-tube solid-phase microextraction (fiber-in-tube SPME) allows direct coupling to HPLC systems for the determination of weakly volatile or thermally labile compounds. This technique utilizes different capillaries and fibers with selective coatings, and has been widely applied in environmental and bioanalytical studies.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Chemical
Xiaowei Li, Wang Chen, Linlin Chen, Guiling Luo, Jing Sun, Yuhong Huang, Kaleab Bizuneh, Yanhong Chao, Wenshuai Zhu
Summary: A simple and effective method for recovering Li+ from high Mg/Li ratio brine at a controlled temperature is proposed in this study. The liquid-liquid extraction of Li+ from high Mg/Li ratio brine is carried out using tributyl phosphate (TBP) diluted in a temperature-responsive ionic liquid. The introduction of the ionic liquid allows for solid-liquid conversion of the extraction phase by adjusting the temperature, achieving efficient phase separation after liquid-liquid extraction. By optimizing parameters such as pH value, IL dosage, and phase ratio, over 80% lithium recovery from a high Mg/Li ratio solution is achieved. The loaded organic phase is regenerated through HCl stripping, with no impurities or emulsification during the process. This study develops a novel and environmentally friendly method for selective lithium recovery using a switchable temperature-responsive TBP-IL system.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Food Science & Technology
Liulin Wei, Jie Liu, Qian Liu, Xiaomei Chen, Zhiqiang Li, Yidong Xu, Xueyun Gao, Xiaoxiao Lu, Zhian Guo, Jingchan Zhao
Summary: In this study, a convenient and sensitive method for the determination of phthalate esters (PAEs) in plastic bottled water was proposed. The method involved the use of a hybrid monolithic column as the extraction phase for on-line in-tube solid-phase microextraction (IT-SPME) coupled with high-performance liquid chromatography (HPLC). The method showed good linearity, low detection limits, and stable recovery rates. This on-line IT-SPME-HPLC method was sensitive, highly efficient, and operationally convenient.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Yan-Zhen Wang, Peng-Cheng Mei, Pei-Rong Bai, Na An, Jin-Gang He, Jie Wang, Quan-Fei Zhu, Yu-Qi Feng
Summary: Bile acids are important co-metabolites of gut microbiota and the host, maintaining metabolic homeostasis. A new mechanism of bile acid metabolism mediated by gut microbiota has been discovered, providing a new perspective for research on bile acid metabolism and gut metabolome.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Min Wang, Neng-Bin Xie, Ke-Ke Chen, Tong-Tong Ji, Jun Xiong, Xia Guo, Si-Yu Yu, Feng Tang, Conghua Xie, Yu-Qi Feng, Bi-Feng Yuan
Summary: DNA methylation (5mC) is a crucial epigenetic modification in mammals. The commonly used technique for quantitatively detecting 5mC, bisulfite sequencing (BS-seq), may cause DNA degradation. In this study, an engineered A3C sequencing (EAC-seq) method was proposed, which can map 5mC in DNA without bisulfite treatment and at single-base resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: Phosphomonoester metabolites play a crucial role in microbial biosynthesis and energy metabolism. Researchers have developed a LC-MS method using isotope labeling for the comprehensive analysis of phosphomonoester metabolites. They successfully identified 42 phosphomonoester metabolites, including two that were first detected in Saccharomyces cerevisiae. The relative quantification results showed significant differences in the content of these metabolites between lycopene-producible and wild-type S. cerevisiae, and further experiments confirmed the relationship between lycopene production and the activity of a specific pathway.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Chemistry, Analytical
Jun Ding, Yu-Qi Feng
Summary: In clinical metabolomics, the use of mass spectrometry (MS) as an analytical platform is essential for characterizing metabolic disturbances, providing diagnostic evidence, and delivering qualitative and quantitative information of the broad metabolome in clinical samples. This review summarizes recent progress in sample collection, preparation, data acquisition, and normalization, and discusses the applications of metabolomics in biomarker screening, disease phenotyping, and clinical outcome prediction. The future directions of clinical metabolomics are also addressed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Cell Biology
Xiaolei Qiu, Zhuoyue Deng, Meijing Wang, Yuqi Feng, Lintao Bi, Lisha Li
Summary: Piezo ion channel, including Piezo1 and Piezo2, is a mechanosensitive protein on the cell membrane that responds to mechanical forces such as stretch, matrix stiffness, static pressure, and shear stress. These Piezo channels transmit mechanical signals and activate downstream pathways involved in stem cell differentiation, including integrin signaling, ERK1/2 MAPK signaling, Notch signaling, and WNT signaling. Differences in Piezo channel expression have been found in the fate of stem cell differentiation and are associated with developmental diseases. This review provides a comprehensive overview of the structure and function of Piezo channels, their relationship with mechanical signals, and their role in stem cell fate and developmental diseases.
Article
Chemistry, Analytical
Tong-Tong Ji, Neng-Bin Xie, Jiang-Hui Ding, Min Wang, Xia Guo, Ying-Ying Chen, Si-Yu Yu, Yu-Qi Feng, Bi-Feng Yuan
Summary: A new member of the uracil-DNA glycosylase family enzyme (UdgX-H109S) was characterized to selectively cleave uracil-containing single-stranded and double-stranded DNA. Based on this, an enzymatic cleavage-mediated extension stalling (ECES) method was developed for locus-specific detection and quantification of uracil in genomic DNA. The ECES method was proven to be accurate and reproducible in the quantification of uracil in genomic DNA from biological and clinical samples, with a significant decrease in uracil level observed in breast cancer tissues.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Yu-Ning Hu, Jin-Tao Zhan, Pei-Rong Bai, Na An, Jun-Jie Tan, Yan-Zhen Wang, Quan- Fei Zhu, Yu-Qi Feng
Summary: In this study, a click chemistry-assisted mass spectrometry strategy was developed to profile the metabolites of DEHP in rats. By labeling and detecting the metabolites of DEHP, a total of 247 metabolites were successfully screened, including some previously unrevealed metabolites. The discovery of these new DEHP metabolites provides additional insights for understanding the metabolism of DEHP and exploring its toxicity mechanism in the future.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Maria Zorina, Victor V. Dotsenko, Pavel N. Nesterenko, Azamat Temerdashev, Ekaterina Dmitrieva, Yu-Qi Feng, Sanka N. Atapattu
Summary: In this study, phthalylglycyl chloride was proposed as a derivatization agent for the determination of octopamine, adrenaline, and dopamine in urine for the first time. The derivatization process combined reversed-phase liquid chromatography with positive electrospray ionization-high-resolution mass spectrometry. An LC-HRMS method with quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes was developed. The derivatization with phthalylglycyl chloride at pH 6.5 and 30 degrees C resulted in a 95-97% yield of derivatives. The proposed method was successfully applied to the analysis of human urine samples and compared with conventional derivatization procedures using 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
Article
Chemistry, Analytical
Yan-Zhen Wang, Yao-Yu Chen, Xin-Ze Wu, Pei-Rong Bai, Na An, Xia-Lei Liu, Quan-Fei Zhu, Yu-Qi Feng
Summary: Gut microbiota-host co-metabolites play a crucial role in mediating communication between the host and gut microbiota, providing nutrients for host cells and regulating the gut microenvironment, which are associated with various diseases. Analysis of these co-metabolites is essential for understanding the host-gut microbiota interaction.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wen-Xuan Shao, Yi-Hao Min, Wei Chen, Jun Xiong, Xia Guo, Neng-Bin Xie, Shan Zhang, Si-Yu Yu, Conghua Xie, Yu-Qi Feng, Bi-Feng Yuan
Summary: A novel technique called AD-seq was developed for the single-base resolution detection of m(6)A in RNA. It utilizes the selective deamination of adenosine by TadA variant to differentiate m(6)A from A in sequencing, allowing for the accurate identification of m(6)A sites and the understanding of its functions in RNA.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sha Li, Yao-Yu Chen, Tian-Tian Ye, Quan-Fei Zhu, Yu-Qi Feng
Summary: A method combining chemical isotope labeling and LC-MS was developed for simultaneous determination of central carbon metabolism pathway (CCM) intermediates with high coverage and accuracy. By chemical derivatization, all CCM intermediates achieved better separation and accurate quantification in a single LC-MS run. This method was applied for accurate quantification of 22 CCM intermediates in different biological samples, and also for quantification at the single-cell level.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Cheng-Jie Ma, Gaojie Li, Wen-Xuan Shao, Yi-Hao Min, Ping Wang, Jiang-Hui Ding, Neng-Bin Xie, Min Wang, Feng Tang, Yu-Qi Feng, Weimin Ci, Yinsheng Wang, Bi-Feng Yuan
Summary: Researchers developed a method called deaminase-mediated sequencing (DM-seq) to map the presence of 6mA in DNA at single-nucleotide resolution. They found that 6mA is mainly located in the GATC motif in E. coli genome and identified 17 6mA sites in mtDNA of HepG2 cells, all of which are in the heavy strand. DM-seq is expected to be a valuable tool for studying the functions of 6mA in DNA and its potential roles in mitochondria-related human diseases.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Analytical
Yaqi Zhu, Saiyi Zhong, Xitian Peng, Qiongwei Yu, Yuqi Feng
Summary: A simple and sensitive method combining solid-phase extraction (SPE) and high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was developed for the determination of benzo[a]pyrene (BaP) in fish. Loofah sponge (LS) was carbonized and used as an SPE adsorbent. The optimized method showed good accuracy and precision with a wide linear range and low detection limit, making it a low-cost and environmentally friendly alternative for the determination of BaP in aquatic products.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Min Wang, Neng-Bin Xie, Ke-Ke Chen, Tong-Tong Ji, Jun Xiong, Xia Guo, Si-Yu Yu, Feng Tang, Conghua Xie, Yu-Qi Feng, Bi-Feng Yuan
Summary: DNA methylation (5mC) is an important epigenetic modification in mammals and its roles are deciphered by detecting 5mC at single-base resolution. Bisulfite sequencing (BS-seq) is commonly used for mapping 5mC but causes DNA damage. Here, we engineered the A3C protein to develop an engineered A3C sequencing (EAC-seq) method, which can quantitatively map 5mC without bisulfite treatment. The EAC-seq approach, employing mild reaction conditions, is a valuable tool for the quantitative detection of 5mC at single-base resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: We developed an LC-MS method using isotope reagents for the comprehensive analysis of phosphomonoester metabolites. This method allowed for rapid screening, identification, and relative quantification of the metabolites in Saccharomyces cerevisiae. We identified 42 phosphomonoester metabolites, including two that were detected for the first time in S. cerevisiae. The relative quantification results revealed significant differences in certain metabolites between lycopene-producible and wild-type S. cerevisiae, indicating a close relationship between phosphomonoesters and lycopene production.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
