Article
Chemistry, Multidisciplinary
Chenlu Pu, Hongli Zhao, Yayun Hong, Zhenxing Wang, Yu Zheng, Minbo Lan
Summary: The study introduces a polydopamine-induced oriented growth strategy to fabricate mesoporous metal oxides with controllable orientation, and demonstrates significantly enhanced phosphopeptide enrichment performance of the constructed 3D and 2D structures.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Cell Biology
Jiaran Li, Jifeng Wang, Yumeng Yan, Na Li, Xiaoqing Qing, Ailikemu Tuerxun, Xiaojing Guo, Xiulan Chen, Fuquan Yang
Summary: In this study, we comprehensively evaluated different TiO2-based phosphopeptide enrichment and fractionation methods, providing a basis for selecting the proper protocols for comprehensive phosphoproteomics.
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yumi Kwon, Seonjeong Lee, Narae Park, Shinyeong Ju, Sungho Shin, Seongjin Yoo, Hankyul Lee, Cheolju Lee
Summary: Phosphorylation is a key component of cellular signaling cascades, controlling important cellular functions. However, phosphoproteomic analysis is typically limited by the amount of sample required for phosphopeptide enrichment. In this study, a new strategy was developed to increase phosphoproteomic signals from a small amount of sample without phosphoenrichment.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Marcos Edgar Herkenhoff, Luiz A. Bovolenta, Oliver Broedel, Lucilene D. dos Santos, Arthur C. de Oliveira, Luiz G. A. Chuffa, Amanda de O. Ribeiro, Luiz A. Lupi, Marco A. D. Dias, Alexandre W. S. Hilsdorf, Marcus Frohme, Danillo Pinhal
Summary: This study characterized the miRNA and protein profiles of Nile tilapia crossbreed using high throughput sequencing and mass spectrometry techniques, revealing distinctive features in gene expression and protein composition between the crossbreed and its parental strains. These unique miRNA and protein signatures contribute to the phenotype of the crossbreed.
Article
Chemistry, Analytical
Shuang Wu, Hui-Min Li, YI-Fan Bing, Yan Zheng, Wen-Lan Li, Xiang Zou, Zhong-Yuan Qu
Summary: The paper investigates the antidepressant quality markers (Q-markers) of Bupleurum scorzonerifolium (BS) using UPLC-ESI-Q-TOF-MS. SSa, SSc, and SSd were confirmed as Q-markers of BS, and the contents of these markers in 10 batches of BS from different origins were determined. This study provides a reliable method for the quality evaluation of BS for depression treatment.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Andrey A. Kuzin, Galina S. Stupnikova, Polina A. Strelnikova, Ksenia V. Danichkina, Maria I. Indeykina, Stanislav I. Pekov, Igor A. Popov
Summary: The study of molecular mechanisms of Alzheimer's disease is crucial for identifying therapeutic targets and early markers. This paper proposes a mass spectrometry-based method for quantitatively assessing specific phosphorylation modifications of Alzheimer's disease-related proteins, with suitable sensitivity for biological samples.
Article
Chemistry, Applied
Qingxi Ren, Fang Guo, Fei Teng, Ying Ma
Summary: By fractionating and analyzing TAG with different melting points, the study successfully reconstructed milk fat globules with excellent microstructural stability and emulsifying activity.
Article
Chemistry, Analytical
Fabio de Souza Barbosa, Leonardo Capra Pezzi, Favero Reisdorfer Paula, Sarah Eller, Elfrides E. S. Schapoval, Tiago Franco de Oliveira, Andreas S. L. Mendez
Summary: The stability of the antibiotic doripenem was evaluated by LC-ESI-Q-TOF method, revealing six degradation products under stress conditions and the formation of a major degradation product through cleavage of the beta-lactam ring under hydrolytic conditions. Additionally, oxidation of the drug using H2O2 led to the formation of three new degradation products. The study provides insight into the degradation behavior of doripenem and its important antibiotic class.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Narendra Kumar Nagendla, Kathirvel Muralidharan, Malothu Raju, Harshavardhan Mohan, Piramanayagam Selvakumar, Murali Mohan Bhandi, Mohana Krishna Reddy Mudiam, Vaikundamoorthy Ramalingam
Summary: Comprehensive metabolic profiling of Mangifera indica leaf extracts revealed their potential therapeutic application in tissue engineering and regenerative medicine. In vitro studies demonstrated that the extracts promote myoblast cell proliferation and induce myotube formation through oxidative stress. Western blot analysis confirmed the upregulation of myogenic marker proteins, indicating myogenic differentiation. In vivo studies showed that the extracts expedite acute wound repair and improve blood perfusion. The leaves of M. indica can be considered as an excellent therapeutic agent for tissue repair and wound healing applications.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Qing Hou, Yuting Huang, Linhong Jiang, Kai Zhong, Yina Huang, Hong Gao, Qian Bu
Summary: Ascidians are marine sources with unique bioactive compounds and high nutritive content, mainly consisting of glycerophospholipids and glycerolipids. They could potentially be used as health-promoting food for humans due to their high levels of glycerophospholipids.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Nanoscience & Nanotechnology
Biplab Mondal, Kingshuk Basu, Rajkumar Jana, Partha Mondal, Biswanath Hansda, Ayan Datta, Arindam Banerjee
Summary: Newly synthesized blue-emitting few-atom copper nanoclusters (CuNCs) have been used as catalysts for the formation of C(sp(2))-C(sp(2)) and C(sp(2))-N(sp(3)) bonds. These copper nanoclusters exhibit high catalytic activity and can replace conventional metal nanoparticles and heavy-metal-ion-based organic catalysts. Computational studies and MALDI-TOF analysis support the hypothesis and reveal the structure and formation of key intermediates during the catalytic reactions.
ACS APPLIED NANO MATERIALS
(2022)
Article
Construction & Building Technology
Stepanka Kuckova, Gabriela Rambouskova, Petra Junkova, Jiri Santrucek, Pavel Cejnar, Tatiana Anatolievna Smirnova, Ondrej Novotny, Radovan Hynek
Summary: The enrichment of mortar mixtures with natural organic materials has a long history, with protein additives proven to enhance mortar durability and strength. This study aimed to identify and quantify proteins in model mortars with various protein additives using mass spectrometry techniques. The results confirmed the possibility of identifying protein additives in century-old mortars, shedding light on traditional recipes.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Jason William Grunberger, Hamidreza Ghandehari
Summary: Silica nanoparticles (SNPs) have versatile synthetic methods and tunable physicochemical properties, making them promising for biomedical applications. In this study, hollow mesoporous SNPs (HMSNPs) with different layer compositions were fabricated, and their degradation profiles were characterized in simulated body fluid. The results showed that the composition and number of layers influenced degradation rates, with particles containing more disulfide bridges being more responsive to enzymatic degradation. These findings suggest the potential utility of layer-by-layer HMSNPs for controlled drug delivery.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).