Article
Chemistry, Analytical
Oana-Maria Istrate, Lucian Rotariu, Camelia Bala
Summary: This study presents a novel L-lactate biosensor based on the immobilization of L-lactate dehydrogenase enzyme on a screen-printed electrode modified with a ternary composite. The biosensor demonstrates good sensitivity and a low detection limit, allowing for the measurement of L-lactic acid in dairy products and wine samples.
Article
Electrochemistry
Sofiia Tvorynska, Jiri Barek, Bohdan Josypcuk
Summary: A cost-effective and reliable biosensing system for rapid and selective determination of L-lactic acid (LA) was developed, which is an essential compound in the winemaking process and considered a significant biomarker for a wide range of diseases. The biosensor architecture includes an easily replaceable lactate oxidase-based mini-reactor connected to a silver amalgam screen-printed electrode acting as a transducer. The use of a spatially separated biorecognition and detection part allowed for the immobilization of a large amount of enzyme, ensuring excellent operational and storage stability.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Mustafa Sen, Merve Oguz, Ipek Avci
Summary: A non-toxic, flexible, low-cost, and disposable multiwalled carbon nanotube (MWCNT)-based screen-printed electrode (SPE) was developed for non-invasive health monitoring applications. The electrode demonstrated excellent electrochemical behavior and is expected to contribute to wearable sensor technology.
Article
Engineering, Electrical & Electronic
Sarah Tonello, Tiziano Fapanni, Stefano Bonaldo, Giada Giorgi, Claudio Narduzzi, Alessandro Paccagnella, Mauro Serpelloni, Emilio Sardini, Sandro Carrara
Summary: In recent years, there has been increasing interest in wearable devices for continuous monitoring of biochemical profiles in patients and athletes, with lactate being one of the most interesting markers for studying fatigue processes. This study proposes a new, low-cost, rapid, and flexible electrochemical amperometric lactate carbon biosensor fabricated using aerosol jet printing (AJP). The biosensors were combined with a paper-based microfluidic system and chitosan-based stable functionalization for long-term continuous monitoring of human activities. The sensor performances were evaluated through static and dynamic electrochemical tests, showing promising results for the development of standalone wearable patches for noninvasive lactate measurements.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Analytical
Nitish Kumar, Yi-Jie Lin, Yu-Chiao Huang, Yu-Te Liao, Shu-Ping Lin
Summary: We have developed an optimal enzyme-based biosensor for detecting lactate concentrations in different fluids. The biosensor utilizes a lactate dehydrogenase (LDH)-modified screen-printed carbon electrode (SPCE) and showed a broad dynamic range and high sensitivity. We also integrated the LDH-modified SPCE into a portable bioelectronic platform for lactate detection in human sweat, which could be used for early diagnosis or real-time monitoring during physical activities.
Article
Chemistry, Applied
A. Smart, A. Crew, O. Doran, J. P. Hart
Summary: This paper presents the development of a novel trilinolein biosensor using lipase and lipoxygenase immobilized on a cobalt phthalocyanine screen-printed carbon electrode. The biosensor demonstrates selective response to polyunsaturated fatty acid (PUFA) triglycerides, distinguishing them from monounsaturated (MUFA) and saturated fatty acid (SFA) triglycerides. The biosensor shows a linear range of 0.2-10 μM and a detection limit of 45.5 nM. It has been successfully applied to measure trilinolein in a pharmaceutical food supplement with a recovery rate of 86% and a coefficient of variation of 5.05%. This simple and cost-effective LOX-lipase biosensor shows great potential for various applications, including food and clinical samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Food Science & Technology
Monika Kundu, Rajesh, Prameela Krishnan, Sumana Gajjala
Summary: This study fabricated biosensors and enzymatic optical biosensors for detecting carcinogenic formaldehyde, with high sensitivity and detection limits. Comparing the electrochemical sensing results with enzymatic optical sensing results on the same sample, there was a high correlation, laying the foundation for further development of biosensors for detecting adulterants and contaminants along the food supply chain.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tzong-Jih Cheng, Hsien-Yi Hsiao, Pei-Chia Tsai, Richie L. C. Chen
Summary: Electrochemical impedance spectroscopy (EIS) is a sensitive analytical method for surface and bulk properties. Here, we demonstrated redoxless ECS for directly determining surfactant adsorption on screen-printed carbon electrodes (SPCEs), providing excellent capacitance plot loci for quantifying the interfacial permittivity of dielectric layers on electrode surfaces. Our proposed approach may serve as a starting point for future probeless and label-free biosensors based on electrochemistry, eliminating disturbance with surface charge properties and minimizing forced potential bias by avoiding redox couples.
Review
Engineering, Electrical & Electronic
Yaping Dong, Yiqun Liu, Jicheng Lv, Li Yang, Yue Cui
Summary: Kidney disease is a leading cause of death worldwide, and creatinine serves as a key biomarker for it. Amperometric biosensors have gained attention for their rapid, sensitive, and selective detection capabilities. The miniaturized and portable nature of these biosensors makes them popular for various settings. This review explores the development of amperometric biosensing for creatinine, including mechanisms, materials, applications, and commercial status, and can be a valuable resource for future creatinine biosensor development and practical applications in kidney patients.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Analytical
Cong Wang, Tan Wang, Zhen Li, Xianbao Xu, Xiaoshuan Zhang, Daoliang Li
Summary: A SPEC/AuNPs/PMB modified electrode was prepared and used for indirect measurement of ammonia nitrogen content in water samples. The biosensor exhibited good sensitivity and stability under certain conditions.
Article
Chemistry, Analytical
Yaping Dong, Xiaojin Luo, Yiqun Liu, Cunling Yan, Haixia Li, Jicheng Lv, Li Yang, Yue Cui
Summary: A disposable creatinine biosensor, based on Prussian blue/carbon-graphite paste and immobilized enzymes, was developed for clinical evaluation of renal function detection. The biosensor showed a linear detection range and high stability, and yielded comparable results to the Jaffe method. This biosensor holds great potential for medical and healthcare applications.
Article
Chemistry, Analytical
Jiaqi Gao, Zhongqiu Hua, Shu Xu, Hao Wan, Zinan Zhi, Xinyi Chen, Shurui Fan
Summary: An amperometric gas sensor using room temperature ionic liquid electrolyte and screen-printed electrodes was developed in this study. The sensor demonstrated rapid response speed, high sensitivity, and wide operating temperature range, making it suitable for use in extreme environments.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Olha Demkiv, Galina Gayda, Nataliya Stasyuk, Anna Moroz, Roman Serkiz, Asta Kausaite-Minkstimiene, Mykhailo Gonchar, Marina Nisnevitch
Summary: In this study, highly sensitive biosensors were developed for the determination of L-Lactate using flavocytochrome b(2) as a bio-recognition element and electroactive nanoparticles for enzyme immobilization. These biosensors exhibited high sensitivity, fast responses, and low limits of detection. The results showed a high correlation between the L-Lactate content determined by the biosensor and referenced enzymatic-chemical photometric methods. These biosensors based on Fcb(2)-mediated electroactive nanoparticles have potential applications in food control laboratories.
Article
Biotechnology & Applied Microbiology
Nan Wang, Guiying Pan, Shimin Guan, Shaofeng Rong, Dapeng Wang, Zhiyong Gao, Peng Tian, Qianqian Li
Summary: In this study, an electrochemical biosensor based on a screen-printed carbon electrode was developed for the rapid detection of human noroviruses (HuNoVs). The biosensor showed high sensitivity and specificity, and could detect different genogroups and genotypes of HuNoVs. The detection limit of HuNoVs in clinical fecal samples was 10(4) genomic copies/mL. The results could be obtained within 1 hour.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Suntisak Khumngern, Ratchaneekorn Jirakunakorn, Panote Thavarungkul, Proespichaya Kanatharana, Apon Numnuam
Summary: A novel oxidase enzyme sensor with high sensitivity and affinity was developed for glucose detection in blood plasma. The sensor exhibited good stability, reproducibility, and selectivity, making it a promising tool for glucose analysis in clinical settings.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).