Article
Polymer Science
Carmen R. Tubio, Xabier Valle, Estela Carvalho, Joana Moreira, Pedro Costa, Daniela M. Correia, Senentxu Lanceros-Mendez
Summary: Poly(hydroxybutyrate-co-hidroxyvalerate) (PHBV) is a biodegradable polymer that can be used as a substitute for plastics made from fossil resources. By blending PHBV with poly(caprolactone) (PCL) and poly(lactic acid) (PLA), its properties can be improved, including morphology, wetting, structure, thermal, mechanical, and electrical characteristics. The addition of PCL and PLA also enhances the antibacterial activity of PHBV. This study demonstrates the potential of developing PHBV blends with PCL or PLA for specific applications.
Article
Chemistry, Multidisciplinary
Christian Bruetting, Julia Dreier, Christian Bonten, Volker Altstaedt, Holger Ruckdaeschel
Summary: Blending polylactic acid (PLA) with poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a practical approach to enhance the foaming behavior of PLA. PHBV is biodegradable, nontoxic, biocompatible, and naturally produced by bacteria. This study investigates the impact of PLA/PHBV blends on sorption behavior, crystallization behavior, dynamic mechanical properties, and autoclave foaming. Results show that PHBV and PLA are immiscible, and the foaming behavior of PLA is correlated with PHBV content, with increased foam density, smaller cell size, and higher foam cell nucleation rate observed as PHBV content increases. The study demonstrates that PHBV can improve the foam morphology of PLA-based foams.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Environmental Sciences
Zubeen J. Hathi, Md Ariful Haque, Anshu Priya, Zi-hao Qin, Shuquan Huang, Chun Ho Lam, Dimitris Ladakis, Chrysanthi Pateraki, Srinivas Mettu, Apostolis Koutinas, Chenyu Du, Carol Sze Ki Lin
Summary: Dependency on plastic commodities has led to an increase in global plastic production. The COVID-19 pandemic has increased medical waste and disrupted the supply chain of personal protective equipment. This study used food waste to cultivate C. necator and produce the biopolymer PHBV through fermentation, addressing the global food waste burden.
ENVIRONMENTAL RESEARCH
(2022)
Article
Polymer Science
Rushabh Patel, Davide Monticone, Mingyuan Lu, Lisbeth Grondahl, Han Huang
Summary: The long-term hydrolytic degradation of SLS PHBV bone scaffolds was explored by incubating them in PBS solution for 20 weeks. The degradation occurred predominantly by bulk degradation, leading to reduction in molecular weight and compressive stiffness while increasing crystallinity. The weight loss was minimal in the first 8 weeks, with gradual increase thereafter, and the concentration of COOH groups on the scaffold surface initially increased before declining due to surface erosion from hydrolysis.
POLYMER DEGRADATION AND STABILITY
(2021)
Article
Polymer Science
Jingjing Mai, Steven Pratt, Bronwyn Laycock, Clement Matthew Chan
Summary: This study explores the synthesis of industrially relevant high-molecular-weight block copolymers PHBV-b-PHBV using high molecular weight hydroxy-functionalised PHBV starting materials coupled with facile diisocyanate chemistry. Two-step synthesis approach and one-step approach were compared, and both successfully produced block copolymers. However, the two-step synthesis was less effective in building molecular weight. Both synthetic approaches were affected by additional isocyanate reactions resulting in the formation of by-products, affecting the product performance.
Article
Polymer Science
Beatriz Melendez-Rodriguez, Sergio Torres-Giner, Maria A. M. Reis, Fernando Silva, Mariana Matos, Luis Cabedo, Jose Maria Lagaron
Summary: A new P(3HB-co-3HV-co-3HHx) terpolyester synthesized using fruit pulp as feedstock was melt-mixed with commercial PHBV to create miscible films for food packaging. As the content of P(3HB-co-3HV-co-3HHx) increased, the mechanical response of the films transitioned from brittle to ductile, with increased permeabilities to gases.
Article
Biochemistry & Molecular Biology
Young Yun Jo, Soyoung Park, Gyeongtaek Gong, Soonjong Roh, Jin Yoo, Jung Ho Ahn, Sun-Mi Lee, Youngsoon Um, Kyoung Heon Kim, Ja Kyong Ko
Summary: This paper reports an improved artificial pathway for enhancing the 3HV component during PHBV biosynthesis from a structurally unrelated carbon source by Cupriavidus necator H16. By genetically manipulating the branched-chain amino acid pathways, a recombinant strain was developed to increase the intracellular accumulation of propionyl-CoA, a key precursor of the 3HV monomer. This recombinant strain produced 42.5% (g PHBV/g dry cell weight) PHBV with 64.9 mol% 3HV monomer from fructose as the sole carbon source.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Hu Qiao, Abderrahim Maazouz, Khalid Lamnawar
Summary: The present work aimed to investigate how the composition and physico-chemical properties affect the rheology, morphology, miscibility, and thermal stability of poly(lactic acid) (PLA)-poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) biopolymer blends obtained by melt mixing. The study revealed the thermo-rheological complexity of the PLA/PHBV blends in the melt, especially for PLA-rich blends, which exhibited irregularly structured morphologies due to highly mismatched viscoelastic properties. The miscibility of the blends was also affected by the lower thermal stability of PLA and PHBV. However, the melt-induced degradation process of the blends seemed to improve their miscibility and thermo-rheological simplicity to some extent.
Article
Engineering, Environmental
Jingjing Mai, Christopher J. Garvey, Clement Matthew Chan, Steven Pratt, Bronwyn Laycock
Summary: Polyhydroxyalkanoates (PHAs) are attractive alternatives to petroleum-derived plastics, as they are derived from bacteria, thermoprocessible, and truly biodegradable. However, the common short chain length PHAs, such as poly(3-hydroxybutyrate) (P3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are relatively stiff and brittle. This study demonstrates the synthesis and characterization of novel high molecular weight PHBV-b-PHBV-b-PHBV block copolymers, which show significantly improved mechanical properties compared to the starting materials. This approach offers an attractive platform for the production of biodegradable PHA materials for various applications.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Engineering, Environmental
Mohammad I. Ibrahim, Diya Alsafadi, Khalid A. Alamry, Mahmoud A. Hussein
Summary: PHBV, a biopolyester with biodegradability, biocompatibility, and being biobased, shows great potential to replace conventional non-degradable polymers. Its weaknesses in mechanical properties and processability drive research towards developing composite materials for various applications.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Changfeng Miao, Dong Meng, Yuling Liu, Fang Wang, Lu Chen, Zhaosong Huang, Xiangyu Fan, Pengfei Gu, Qiang Li
Summary: This study identified a phaC(R) gene encoding PHA synthase in Rhodoligotrophos defluvii, and successfully synthesized different content and structural PHA copolymers under different carbon source conditions, achieving efficient biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Sara Alfano, Francesca Pagnanelli, Andrea Martinelli
Summary: Polyhydroxyalkanoates (PHAs) are degradable polyesters with the potential to replace oil-based polymers. This study presents a new method using attenuated total reflection FTIR (ATR-FTIR) to rapidly determine the composition of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV), the most common PHA copolymer. The method proves to be useful for easy and rapid estimation of P3HBV composition.
Article
Biochemistry & Molecular Biology
Beatriz Melendez-Rodriguez, Maria A. M. Reis, Monica Carvalheira, Chris Sammon, Luis Cabedo, Sergio Torres-Giner, Jose Maria Lagaron
Summary: This study developed three new copolymers of PHBV with different 3HV contents using cheese whey as feedstock. The resultant PHBV biopapers showed high transparency but slightly yellow color. The 40% 3HV sample exhibited the highest ductility and toughness among the three copolymers.
Article
Biochemistry & Molecular Biology
Isabela F. Santos, Rodolfo M. Moraes, Simone F. Medeiros, Jaspreet K. Kular, Marcus A. Johns, Ram Sharma, Amilton M. Santos
Summary: Polymeric nanoparticles were developed without any matrix ligands on their surface to promote cell attachment and proliferation of human osteoblasts. The study demonstrated that cells readily attached and spread onto the nanoparticle surfaces compared to non-tissue culture polystyrene, indicating the potential of PHBV nanoparticles for cell attachment and growth in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Multidisciplinary
Silvia Bittolo Bon, Irene Chiesa, Davide Morselli, Micaela Degli Esposti, Paola Fabbri, Carmelo De Maria, Tommaso Foggi Viligiardi, Antonino Morabito, Giacomo Giorgi, Luca Valentini
Summary: This study reports the fabrication of two different three-dimensional architectures of regenerated silk (RS) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with embedded functionalities, which have potential clinical applications. Experimental data are supported by finite element simulations, demonstrating the possibility to program the shape-changing behavior of 3D printed structures. The synergic effect obtained by combining materials on appropriate architectures paves the way to potential clinical applications.
MATERIALS & DESIGN
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).