Article
Biochemistry & Molecular Biology
Dalma Dojcsak, Agnes Maria Ilosvai, Laszlo Vanyorek, Ibolya Gilanyi, Csaba Olah, Laszlo Horvath, Csaba Varadi
Summary: Glycosylation, essential for proper functioning of glycoproteins, is known to be altered in chronic inflammatory disorders such as multiple sclerosis. The use of magnetic nanoparticles in glycan sample preparation provides an easy-to-use solution for large-scale biomarker discovery studies. Analyzing serum samples from multiple sclerosis patients and healthy controls, significant glycosylation alterations were found, particularly in different gender groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Applied
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: This review summarizes the recent research progress on MALDI mass spectrometry (MS) detection in food carbohydrates, including the improvement of ionization efficiency, the application in structure characterization of polysaccharides, and the quantitative study and mass spectrometry imaging.
Article
Chemistry, Multidisciplinary
Xiwei Wang, Lu Yan, Zijing Yu, Qiaoji Chen, Mingshu Xiao, Xiaohui Liu, Li Li, Hao Pei
Summary: This study presents a novel sensing platform based on fractal gold nanoflowers for selective analysis of metabolites in complex biofluids without tedious sample pretreatment. The platform shows potential applications in disease diagnosis and therapeutic evaluation.
Article
Biochemical Research Methods
Canhong Zhu, Jiani Wu, Xueting Jin, Yinghua Yan, Chuan-Fan Ding, Keqi Tang, Qiaohong Zhang
Summary: The hydrophilic graphene composite functionalized with glutathione and L(+)-Cysteine, named G@S@Au@GC, shows excellent performance in glycopeptide adsorption, with advantages of low cost, simple and fast synthesis, and superior enrichment performance.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Retraction
Chemistry, Multidisciplinary
Feng Ding, Yuna Qian, Zaian Deng, Jitai Zhang, Yongchao Zhou, Lan Yang, Fangyan Wang, Juping Wang, Zhihua Zhou, Jianliang Shen
Summary: The study on size-selected silver nanoparticles for MALDI-TOF mass spectrometry of amyloid-beta peptides by Feng Ding et al. was retracted from Nanoscale journal in 2018.
Review
Chemistry, Analytical
Sebastien Allard, Frants R. Lauritsen
Summary: This review summarizes the progress in the use of MIMS for DBP analysis in water since 2010. It highlights advancements in instrumentation, quantification, and new applications in swimming pools, water treatment, mechanistic studies, and kinetic modelling. The review discusses the use of high resolution mass spectrometry for distinguishing between isobaric isomers, analysis of hydrophilic and less volatile DBPs using condensed phase MIMS, and quantification based on univariate and multivariate mathematical algorithms. MIMS has been proven to be a valuable tool for implementing water treatment strategies focusing on DBP mitigation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Arnaud Cannet, Camille Simon-Chane, Mohammad Akhoundi, Aymeric Histace, Olivier Romain, Marc Souchaud, Pierre Jacob, Pascal Delaunay, Darian Sereno, Philippe Bousses, Pascal Grebaut, Anne Geiger, Chantel de Beer, Dramane Kaba, Denis Sereno
Summary: This study presents a new method for identifying Glossina species using Wing Interference Patterns (WIPs). By combining deep learning algorithms with a database of WIPs representing 23 Glossina species, accurate automatic recognition is achieved.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Roman S. Borisov, Mariya D. Matveeva, Vladimir G. Zaikin
Summary: In recent years, there has been a special focus on the use of reactive matrices in MALDI-MS, which have dual properties and have been applied in bioanalysis to reduce sample treatment time and improve sensitivity. True reactive matrices interact with molecules through exchange or addition reactions, enhancing molecular identification and profiling.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Haiyan Chen, Mengyuan Zhou, Yuteng Zeng, Tongtong Miao, Haoyuan Luo, Yang Tong, Mei Zhao, Rui Mu, Jiang Gu, Shudi Yang, Liang Han
Summary: This study proposes a biomimetic nanoparticle that utilizes the BBB invasion ability of Escherichia coli K1 (EC-K1) outer membrane for brain-targeted drug delivery. The nanoparticles penetrate the BBB through the transcellular vesicle transport pathway, which is mediated by the interactions between outer membrane protein A and gp96 on BBB endothelial cells. This nanoengineering strategy provides the loaded drugs with prolonged circulation, intracranial interstitial distribution, and extremely high biocompatibility. The study also reveals the potential of delivering therapeutics to treat gp96-overexpressing intracranial malignancies.
Article
Chemistry, Applied
Yunhe Hong, Nicholas Birse, Brian Quinn, Yicong Li, Wenyang Jia, Saskia van Ruth, Christopher T. Elliott
Summary: In this study, the difference between wild and farmed salmon production was successfully characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) combined with chemometric analysis. The established method involved multivariate analysis techniques, such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), to identify distinctive features in wild and farmed salmon products. The proposed method achieved 100% classification accuracy in discriminating farmed and wild salmon, as well as distinguishing different species of wild salmon. The results suggest that this method could be used as a reference for detecting salmon fraud related to production type and expand the application of MALDI-ToF technology in food authenticity testing.
Article
Chemistry, Analytical
Faisal Faiz, Jun-qin Qiao, Hong-zhen Lian, Li Mao, Xiao-bing Cui
Summary: The study utilized mercapto- and amino-functionalized magnetic nanoparticles as sorbents for magnetic solid-phase extraction of arsenite and arsenate, followed by ICP-MS detection. Through optimization of various parameters, the combined scheme demonstrated excellent selectivity and anti-interference ability, achieving satisfactory results for the extraction and detection of both arsenic species.
Article
Biochemistry & Molecular Biology
Marco Roverso, Roberta Seraglia, Raghav Dogra, Denis Badocco, Silvia Pettenuzzo, Luca Cappellin, Paolo Pastore, Sara Bogialli
Summary: This study evaluated the use of single-walled carbon nanohorns (SWCNH) as a specific surface for Surface-Assisted Laser Desorption Ionization (SALDI) analysis. The optimized method provided rapid analysis of amino acids and lipids, and accurately determined the composition of fatty acids in olive oil samples without any sample treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kristine Korzow Richter, Maria C. Codlin, Melina Seabrook, Christina Warinner
Summary: Collagen peptide mass fingerprinting by MALDI-TOF-MS, also known as ZooMS, is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. It enables rapid taxonomic identification of large bone assemblages and cultural heritage objects. However, there are limitations that need to be addressed for future advancements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Oncology
Rita Casadonte, Mark Kriegsmann, Katharina Kriegsmann, Isabella Hauk, Rolf R. Meliss, Cornelia S. L. Mueller, Joerg Kriegsmann
Summary: This study utilized imaging mass spectrometry to compare the proteomic profiles of cutaneous melanomas and melanocytic nevi in a large cohort, identifying a signature that can distinguish malignant melanoma from benign nevus with an overall accuracy of over 98%. The prediction model was validated in an independent set with an accuracy of 93% in classifying melanoma from nevi. Statistical analysis of the imaging mass spectrometry data revealed significant variations in mass-to-charge ratio peaks between the two tissue types. This study represents the largest imaging mass spectrometry investigation of cutaneous melanoma and nevi to date, demonstrating the potential for IMS to discriminate between melanocytic nevi and melanoma.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).