Article
Chemistry, Analytical
Ziyan Yu, Yaxiao Liao, Jie Liu, Qin Wu, Yu Cheng, Ke Huang
Summary: A novel colorimetric approach for detecting kanamycin based on the aggregation of gold nanoparticles (AuNPs) induced by kanamycin is presented in this study. The sensor showed a linear range of 0.5-3 nM and a limit of detection of 0.11 nM under optimal conditions. It was tested by monitoring kanamycin in six food samples with satisfactory spiked recoveries, making it a desirable choice for fast and highly sensitive detection of kanamycin.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yuling Li, Bowen Zhou, Keke Wang, Jing Zhang, Wenjian Sun, Li Zhang, Yinlong Guo
Summary: The mass-tagged chiral selector [d(0)]/[d(5)]-17 beta-EBC was successfully developed for the separation and identification of amino acid enantiomers, achieving good enantiomeric resolution for 19 chiral proteinogenic AAs in a quick analytical run. The linear calibration curve of enantiomeric excess using [d(0)]/[d(5)]-17 beta-EBC showed promising results, demonstrating its potential in peptide drug quality control and discovery of chiral disease biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Justyna Piechocka, Rafal Glowacki
Summary: This overview summarizes all documented analytical methods for the quantification of homocysteine thiolactone (HTL) in biological samples, with a focus on pre-analytical treatment strategies and separation techniques. The review also discusses the advantages, pitfalls, and limitations of the methods, as well as the origin and main metabolites of HTL, and the association between civilization diseases and sulfur-containing compounds' metabolism disorders.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Apostolia Tsiasioti, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: This article reviews recent studies on the determination of histamine in food matrices using derivatization-based separation methods. The derivatization of histamine plays an important role in enhancing sensitivity, selectivity, and compatibility to analytical techniques. The article also discusses the application of the latest derivatizing reagents for histamine and various analytical techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Katerina Cokrtova, Vit Mares, Tomas Krizek
Summary: The feasibility of on-capillary derivatization of saccharides by aromatic amine-based fluorescent labeling agents was tested. Glucose was used as a model analyte and the direct reaction of the saccharide with the labeling agent in acetic acid yielded a stable labeled product that could be separated and detected effectively. The method showed excellent linearity and repeatability for a mixture of different types of saccharides.
Article
Chemistry, Analytical
Natalia Kaczmarczyk, Julita Cizewska, Natalia Treder, Natalia Miekus, Alina Plenis, Piotr Kowalski, Anna Roszkowska, Tomasz Baczek, Ilona Oledzka
Summary: This study evaluates the impact of using ionic liquids (ILs) on the separation efficiency of biogenic amines (BAs) in micellar electrokinetic chromatography (MEKC). It found that ILs with chloride and tetrafluoroborate ions were the most effective, while ILs with trifluoromethanesulfonate and bis(trifluoromethylsulfonyl)imide ions disrupted the separation process. Overall, this study provides a critical assessment of the different types and concentrations of ILs in MEKC analysis of selected BAs.
Article
Chemistry, Analytical
Shuo Liu, Xin Li, Xifeng Yang, Linhui Zhou, Xiangfeng Liang, Ruchen Qiu, Yun Fa
Summary: A method for simultaneous determination of six monosaccharides using pre-column derivatization and capillary electrophoresis was developed. The optimized conditions resulted in low detection limits, high correlation coefficients, and good recoveries. This method has potential applications in the analysis of free monosaccharides in natural products.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Samantha Goncalves, Luciano Molognoni, Heitor Daguer, Ricardo Pimenta, Rodrigo Barcellos Hoff, Gustavo Amadeu Micke, Luciano Vitali
Summary: This study developed an innovative method for sulfite inspection in seafood using capillary zone electrophoresis. The method is simple, fast, and allows for simultaneous extraction and deriva-tization of sulfite, suitable for high throughput analysis. The method showed selectivity and specificity when compared to liquid chromatography coupled to mass spectrometry. The results met the required parameters for metrological accreditation.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Nora Gampe, Lilla Ladocsi, Ida Fejos, Imre Boldizsar, Andras Darcsi, Szabolcs Beni
Summary: Non-protein amino acids, important metabolites of the Fabaceae family, have valuable biological effects. A chiral separation method was developed for homoproline and homopipecolic acid enantiomers and applied to plant sample analysis. The presence of these amino acids was confirmed in Fabaceae species, with significant variations observed between species, geographical origins, and botanical parts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Denisa Smolkova, Richard Cmelik, Jana Lavicka
Summary: Capillary electrophoresis is widely used for oligosaccharide and glycan analysis, but the low electrophoretic mobility and lack of chromophore or fluorophore in saccharide molecules require modification before separation and detection. This review summarizes the labeling strategies used for oligosaccharide/glycan analysis, categorized based on the labeling mechanism and saccharide molecule. In addition to the commonly used 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, less common and recently developed tags applicable in glycomics are also summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Bathinapatla Ayyappa, Suvardhan Kanchi, Myalowenkosi Sabela, Krishna Bisetty
Summary: A novel background electrolytic system was applied successfully for the separation of sucralose using indirect UV detector with capillary electrophoresis. Optimization of BGE, UV detection wavelength, buffer concentration, buffer pH, cassette temperature, and applied voltage enhanced the sensitivity and selectivity of the separation method, leading to successful determination of sucralose in food samples.
CURRENT ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Azam Gholizadeh, Kathleen Black, Howard Kipen, Robert Laumbach, Andrew Gow, Clifford Weisel, Mehdi Javanmard
Summary: This study focuses on the standardization of a portable nitrite sensor, which is a key biomarker for inflammation in the respiratory tract. The storage of EBC samples and electrical properties of the sensor were investigated. The sensor's performance was evaluated using differential pulse voltammetry and compared with a chemiluminescence method. The study also characterized the electrical behavior of the sensor and electrical variations between EBC samples. These findings contribute to the development of portable and on-site point-of-care sensors for detecting nitrite in untreated EBC samples.
Article
Biochemistry & Molecular Biology
Tong Li, Ruiguo Wang, Peilong Wang
Summary: Biogenic amines (BAs) are formed from amino acids and can have various structures. In our study, a method using ultra-performance liquid chromatography-tandem mass spectrometry was developed to rapidly determine 14 BAs in fish samples. The method showed good linearity, low limits of detection, and satisfactory recovery. It was successfully applied to analyze the concentration levels of BAs in different fish.
Article
Food Science & Technology
Adriane Costa dos Santos, Fabiola Carina Biluca, Patricia Brugnerotto, Luciano Valdemiro Gonzaga, Ana Carolina Oliveira Costa, Roseane Fett
Summary: This study investigated the presence of fourteen aliphatic organic acids (AOA) in honeys produced by different species of Brazilian stingless bees and characterized their physicochemical properties. The study found significant differences in physicochemical properties among the samples and identified acetic, gluconic, and lactic acids as the major AOA in all the samples. Further research on the physical and chemical characteristics of honey from different species of stingless bees is needed.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Ya-Ling Yu, Min-Zhen Shi, Si -Chen Zhu, Jun Cao
Summary: An online sandwich derivatization and stacking strategy was developed and applied to the preconcentration of multiple amino acids in functional food samples. The method showed good linearity, precision, and recovery, indicating its potential for determining free amino acids in samples with complex matrices.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).