Article
Engineering, Chemical
Qi Zhang, Siru Ren, Song Xue, Ang Li, Siyao Liu, Xiaodong Sun
Summary: The study evaluated six novel ionic liquids as chiral selectors in capillary electrophoresis, showing better enantioseparation performance compared to traditional chiral selectors.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Mengmeng Ji, Yanhui Zhong, Ming Li, Rongxia Tan, Yufei Hu, Gongke Li
Summary: A CTL method was developed for rapid determination of acetic acid in enzyme products. The NiMn LDH/CNT/GO nanocomposite exhibited excellent CTL activity due to its larger specific surface area and more exposure to active sites. This method showed a linear relationship between CTL response and acetic acid concentration in the range of 0.31-12.00 mg.L-1, with a detection limit of 0.10 mg.L-1. The developed method is rapid, taking only about 13 s, and requires minimal sample preparation. The proposed CTL method has promising potential for quality monitoring of enzymes.
Article
Microbiology
Mengsha Li, Teng Li, Ming Zhou, Mengdi Li, Yexin Zhao, Jingjing Xu, Feng Hu, Huixin Li
Summary: Bacterial-feeding nematodes regulate the interaction between nematodes and bacteria by influencing bacterial IAA biosynthesis. Extracts from Caenorhabditis elegans promote metabolic activity in A. pascens ZZ21 and assist bacteria in resisting stress.
Article
Meteorology & Atmospheric Sciences
Poonam Bikkina, Srinivas Bikkina, Kimitaka Kawamura
Summary: Oxalic acid is the most abundant dicarboxylic acid in the South Asian outflow, indicating less aged organic aerosols. The concentrations of glyoxal and glyoxylic acid are strongly correlated with the concentrations of succinic acid, nss-Ca2+ and EC, suggesting gas/particle partitioning and aqueous phase oxidation reactions.
ATMOSPHERIC RESEARCH
(2023)
Article
Energy & Fuels
Gheorghita Mitran, Adriana Urda, Octavian-Dumitru Pavel, Stefan Neatu, Mihaela Florea, Florentina Neatu
Summary: A series of cobalt-iron mixed oxides were synthesized and tested as catalysts for the oxidative decarboxylation of malic acid. The Co3Fe sample showed the highest conversion and yield, which could be attributed to the increased number of defects caused by the substitution of Co2+ for Fe3+.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Analytical
Pengfei Zhao, Sisi Tang, Xiaodan Wu, Yu Chen, Kangling Tang, Jinli Fu, Huibin Lei, ZhaoXia Yang, Zhaohui Zhang
Summary: The development of a method with micro-volume reagent usage and high selectivity is crucial for rapid determination of valuable disease-related biomarkers. In this study, a novel ratiometric fluorescence capillary imprinted sensor based on luminescent metal-organic framework (UiO-66-NH2) was developed for highly selective fluorescence determination of sialic acid (SA). The sensor successfully detected SA in human serum and saliva samples within a linear concentration range of 1.0 x 10- 7-3.0 x 10-4 M, with a detection limit of 2.5 x 10-8 M. This sensitive and selective fluorescence method with micro-volume sample consumption (15 μL) provides a practical approach for SA determination.
Article
Biochemical Research Methods
Hai Yen Ta, Lucie Perquis, Cedric Sarazin, Bruno Guiard, Varravaddheay Ong Meang, Fabrice Collin, Francois Couderc
Summary: The study utilized capillary electrophoresis coupled to LED-induced fluorescence detection and CBQCA labeling for the analysis of amino acids such as tryptophan, facing challenges in detection and quantification, especially for Trp. Another approach, CE/capacitively coupled contactless conductivity detection, showed better performance for quantification of Trp, Tyr, Val, and Ile, with a LOD of 2 μM for Trp.
Article
Chemistry, Analytical
Sisi Tang, Xiaodan Wu, Pengfei Zhao, Kangling Tang, Yu Chen, Jinli Fu, Shu Zhou, Zhaoxia Yang, Zhaohui Zhang
Summary: This study developed a near-infrared fluorescence capillary imprinted sensor with high selectivity and sensitivity for chiral recognition of L-histidine. The sensor exhibited superior stability, reproducibility, and reusability, displaying high selectivity towards L-histidine in micro-volume samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Fei Qu, Ziwei Guo, Dafeng Jiang, Xian-En Zhao
Summary: The polymerization of dopamine can be efficiently catalyzed by acid phosphatase, enabling its detection in a short period of time. In situ growth of polydopamine on covalent organic frameworks allows for sensitive and selective detection of dopamine without interference from other substances.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Sisi Tang, Pengfei Zhao, Xiaodan Wu, Yu Chen, Kangling Tang, Shu Zhou, Jinli Fu, Huibin Lei, Zhaoxia Yang, Zhaohui Zhang
Summary: A novel dual-emission ratiometric fluorescence capillary imprinted sensor based on luminescence metal organic framework was successfully fabricated, with high sensitivity and selectivity for chiral recognition of L-tyrosine. It showed great potential for detecting L-tyrosine in actual samples with satisfactory recoveries and a low detect limitation. The integration of molecularly imprinted technology and fluorescence capillary method provides a route for trace detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Yongyan Xi, Hongtao Xu, Tao Zhan, Ying Qin, Feiyu Fan, Xueli Zhang
Summary: Currently, the biological production of L-malic acid (L-MA) faces challenges due to the generation of waste salts and environmental hazards. In this study, acid-tolerant yeast Pichia kudriavzevii was engineered to efficiently produce L-MA through low pH fermentation. This was achieved by constructing an L-MA-producing strain and fine-tuning metabolic flux. Overexpression of a pyridine nucleotide transhydrogenase from E. coli helped overcome the insufficient NADH supply and further improved L-MA production. The engineered strain achieved a high L-MA yield and productivity, while reducing the need for neutralizing agents.
METABOLIC ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Pawel Mateusz Nowak, Maria Klag, Gabriela Kozka, Malgorzata Golab, Michal Wozniakiewicz
Summary: This article presents the first successful application of a capillary electrophoresis-microscale thermophoresis tandem technique (CE-MST) for determining equilibrium constant values. The technique combines CE and MST instruments to online determine the pKa value. It provides a way to separate samples and determine pKa value using three independent methods.
Article
Chemistry, Analytical
Tingting Hong, Ronghui Zheng, Lin Qiu, Shuwen Zhou, Hufei Chao, Ying Li, Wen Rui, Pengfei Cui, Xinye Ni, Songwen Tan, Pengju Jiang, Jianhao Wang
Summary: A strategy based on fluorescence coupled capillary electrophoresis (CE-FL) was developed for analyzing tetrahedron DNA (TD) and TD-doxorubicin (DOX) conjugate. The optimized method showed satisfactory repeatability and accuracy. CE-FL is a sensitive and specific method for TD assay, with potential applications in DNA nanomaterials research.
Article
Chemistry, Analytical
Lei Deng, Feng Huang, Aomei Zhang, Tingting Wang, Minghui Yang, Xiaoqing Li, Xiang Chen
Summary: A fluorescent sensor for alkaline phosphatase determination based on CsPbBr3/BSA NCs perovskite was successfully developed by using bovine serum albumin (BSA) as a zwitterion ligand. The sensor showed a linear concentration range of 50 to 500 μM for L-ascorbic acid (AA) and a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, as well as a linear concentration range of 40 to 500 U/L for alkaline phosphatase (ALP) and a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for ALP. Furthermore, the proposed biosensor exhibited good selectivity and recovery in the determination of ALP in human serum.
Article
Agronomy
Diana Laura Medina-Melchor, Diego Helman Zapata-Sarmiento, Elvia Becerra-Martinez, Mario Rodriguez-Monroy, L. Gerardo Zepeda Vallejo, Gabriela Sepulveda-Jimenez
Summary: Infection with Stemphylium vesicarium reduces energy production processes in onion plants while promoting the successful growth of the pathogen by increasing the concentration of certain metabolites.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).