Journal
TALANTA
Volume 74, Issue 5, Pages 1492-1497Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2007.09.026
Keywords
glutathione reductase; sulfhydryl oxidase; reduced/oxidized glutathione; electrochemical detection
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Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O-2 consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 mu M nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD). (C) 2007 Elsevier B.V. All rights reserved.
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