Journal
TALANTA
Volume 76, Issue 5, Pages 1207-1211Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2008.05.027
Keywords
glycogen; cold nanoparticle; resonance light-scattering; plasmon absorption
Categories
Funding
- National Nature Sciences Foundation of China [20475004, 90713013, 20775004]
- Instrumental Analysis Fund of Peking University
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Simple and sensitive methods for the determination of glycogen are desirable. This study was to establish the determination of glycogen based on the enhancement of the light-scattering intensity of gold nanoparticles (AuNPs) at nanogram per milliliter levels. Glycogen enhancement was independent of temperature and was highest at pH 7. The enhancement was linear from 10 to 800 ng mL(-1) with a 2 ng mL-1 detection limit (3 S/N). The standard deviation of the slope for 5 consecutive calibration runs was within 5%. Analysis of three artificial samples with varied glycogen concentrations had 98-102% recovery. Selected amino acids, glucose, lactose, BSA and trichloroacetic acid all showed minor effects on glycogen enhancement demonstrating the potential for glycogen determination in complex samples. (C) 2008 Elsevier B.V. All rights reserved.
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