Ancylobacter dichloromethanicus sp nov - a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane

A novel aerobic facultative methylotroph was isolated from contaminated soil. The organism (strain DM16) is a Gram-negative asporogenous non-motile curved rod multiplying by binary fission. Cells are neutrophilic and mesophilic. This strain utilized dichloromethane, methanol, formate and formaldehyde along with a variety of polycarbon compounds. Strain DM16 employs the ribulosebisphosphate pathway for C-1 assimilation. The DNA G + C content is 64.5 mol%. The major ubiquinone is Q-10. The dominant cellular fatty acids are 18:1 omega 7c (58.6%), cyclo-19:0 omega 8c (34.8%) and 16:0 (3.2%). Sequencing of the 16S rRNA gene and DNA-DNA hybridization experiments clearly indicated that this methylotroph should be classified as a new species within genus Ancylobacter - Ancylobacter dichloromethanicus sp. nov. with the type strain DM 16(T) (DSM 21507(T) = VKM B-2484(T)). (C) 2009 Elsevier GmbH. All rights reserved.