Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Chemistry, Analytical
Ge Ma, Xiaoyong Zhao, Mengzhe Guo, Yaqin Liu, Keqiang Shi, Cheng Guo, Yuanjiang Pan
Summary: In this study, a matrix glycosylation strategy was proposed to improve the sensitivity and signal reproducibility of MALDI MS analysis. The glycosylated matrices, especially 6-GAQ, exhibited remarkably-improved performance in detecting metabolites and imaging small molecules in complex samples. The glycosylation strategy showed great potential in the field of mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Quan He, Cuirong Sun, Jian Liu, Yuanjiang Pan
Summary: This article discusses the role of matrix-assisted laser desorption/ionization mass spectrometry imaging in cancer drug development and therapy, emphasizing its importance in revealing drug distribution and metabolism.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Microbiology
Xin-Fei Chen, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, Po-Ren Hsueh
Summary: MALDI-TOF MS has been used in clinical microbiology since 2010, offering advantages such as convenience, speed, accuracy, and low cost. It has shown promising results in rapid detection of highly virulent and drug-resistant pathogens, making it a valuable tool in clinical microbial identification.
Article
Agriculture, Multidisciplinary
Xin Zhao, Jiayin Lu, Shuping Long, Winnie C. Soko, Qin Qin, Liang Qiao, Hongyan Bi
Summary: A strategy combining IMS and MALDI-TOF MS was developed to test seafood allergens, with consistent results compared to ELISA tests and a 0% false positive rate. The method shows potential for detecting seafood allergens to ensure the safety of allergic patients.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Sophie E. Lellman, Christopher K. Reynolds, A. K. Barney Jones, Nick Taylor, Rainer Cramer
Summary: This study introduces the use of liquid atmospheric pressure MALDI for detecting bovine tuberculosis, using a three-class prediction model and nasal swabs for sample collection, with a short sample preparation time. The results show comparable sensitivity, specificity, and overall accuracy to the traditional skin test, demonstrating the potential of this method as a single-visit diagnostic platform that provides results on the same day of sampling.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Liping Liu, Yaru Jia, Shanxia Jin, Xiangdong Chen, Ping Zhou
Summary: A new strategy for the analysis of small biomolecules and drugs by LDI MS is presented, using in situ polymerized poly(L-dopa) film on standard stainless steel MALDI plate. This method has high sensitivity and good salt tolerance, allowing detection of amino acids even in saturated salt solution, and has the potential to be a practical solution for small biomolecule analysis.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Chemical
Jiawen Wu, Ze Rong, Peng Xiao, Yuanzhe Li
Summary: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an advanced technique used for mass spectrometry analysis by utilizing fragmented ions from complex molecules. It has wide applications in tumor and tissue imaging but has the potential for improvement in detecting larger proteins in certain tissues.
Article
Biochemical Research Methods
Josiah C. McMillen, Danielle B. Gutierrez, Audra M. Judd, Jeffrey M. Spraggins, Richard M. Caprioli
Summary: The study demonstrated the increased sensitivity and protein identification in spatial mapping of tryptic peptides in thin tissue sections of human kidney by employing MALDI-2 laser in addition to conventional MALDI-1. Peptides were identified tentatively based on mass accuracy by matching MALDI peak list to a database of theoretical peptides, resulting in enhanced protein identifications in IMS experiments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)