Journal
SURFACE AND INTERFACE ANALYSIS
Volume 45, Issue 1, Pages 309-311Publisher
WILEY-BLACKWELL
DOI: 10.1002/sia.4903
Keywords
NanoSIMS; arsenic; rice; roots; subcellular localisation
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Funding
- UK EPSRC platform grant [EP/F048009]
- BBSRC [BB/F004087/1] Funding Source: UKRI
- EPSRC [EP/I026584/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F004087/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I026584/1] Funding Source: researchfish
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Determining the distribution of trace elements in biological materials with subcellular resolution is very challenging but vitally important in order to understand their mechanisms of uptake. Rice grain is efficient in the accumulation of arsenic (As) in the grain, potentially posing a severe health risk to millions of people in South-East Asia. The NanoSIMS is a state-of-the-art microscope capable of high resolution chemical imaging (down to 50 nm) and detecting very low elemental concentrations (parts-per-million levels). This makes it ideally suited for trace element localisation in biological materials. This paper shows how the NanoSIMS can be used to investigate the localisation of As in rice grain at high resolution and how different treatments result in different distributions in the grain. The precise location of parts-per-million As concentrations and its localisation with other elements at the subcellular scale in rice roots are also shown. Copyright (C) 2012 John Wiley & Sons, Ltd.
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