Journal
SURFACE AND INTERFACE ANALYSIS
Volume 45, Issue 1, Pages 298-301Publisher
WILEY-BLACKWELL
DOI: 10.1002/sia.5036
Keywords
lipids; C60-QSTAR; KDO2 Lipid A; RAW 264.7; tandem MS
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Funding
- LIPID MAPS Consortium [GM 069338-07]
- National Institutes of Health [2R01 EB002016-16]
- National Science Foundation [CHE-0908226]
- European Research Council
- Knut and Alice Wallenberg Foundation
- Swedish Research Council (VR)
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1212645, 0908226] Funding Source: National Science Foundation
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Although secondary ion mass spectrometry (SIMS) has been successfully employed for mapping lipid distributions at the cellular level, the identification of intact lipid species in situ is often complicated by isobaric interference. The high mass resolution and tandem MS capabilities of a C-60-QSTAR hybrid instrument has been utilized to identify over 50 lipid species from mouse macrophages (RAW 264.7). In this investigation, lipid assignments made based on mass accuracy were confirmed with tandem MS analyses. Data obtained from C-60-SIMS was compared to liquid chromatography (LC)-MS data obtained by the LIPID MAPS consortium. A majority of the lipids detected with LC-MS, but not detected with C-60-SIMS, were present at concentrations below 2.0 pmol/mg of DNA. Matrix-related effects prevented the detection of lipids with the glycerophosphoethanolamine (PE) headgroup, glycerophosphoserine (PS) headgroup and lipids with polyunsaturated fatty acyl chains in the C-60-SIMS analyses. Lipid distributions obtained from a lawn of RAW 264.7 cells stimulated with the endotoxin KDO2-Lipid A were also studied. The results obtained with C-60-SIMS agreed with the established LC-MS data for the glycerophosphoinositol lipid class (PI) with adequate molecular sensitivity achieved with as few as 500 cells. Copyright (C) 2012 John Wiley & Sons, Ltd.
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