Journal
STRUCTURE
Volume 21, Issue 12, Pages 2152-2161Publisher
CELL PRESS
DOI: 10.1016/j.str.2013.09.012
Keywords
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Funding
- Maurice Muller Foundation of Switzerland [SNF 3100AOB-128659, CRSII3_125110]
- Swiss National Science Foundation
- Swiss Initiative for Systems Biology (SystemsX.ch)
- Swiss Federal Government
- Maurice E. Mueller Foundation
- Swiss National Science Foundation (SNF) [CRSII3_125110] Funding Source: Swiss National Science Foundation (SNF)
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The type Ill secretion system machinery, also known as the injectisome, delivers bacterial effector proteins into eukaryotic cells during infection. The outer membrane YscC secretin is a major part of Yersinia enterocolitica's injectisome and is among the first components to assemble, solely assisted by its pilotin, YscW. We have determined the three-dimensional structures of the native complex and its protease-resistant core to 12 A resolution by cryo-electron microscopy (cryo-EM) and show that YscC forms a dodecameric complex. Cryo-EM of YscC reconstituted into proteoliposomes defines the secretin's membrane-spanning region. Native YscC consists of an outer membrane ring connected via a thin cylindrical wall to a conical, periplasmic region that exposes N-terminal petals connected by flexible linkers. These petals harbor the binding site of YscD, a component of the inner membrane ring. A change in their orientation adapts the length of the YscC secretin and facilitates its interaction with YscD.
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