Journal
STRUCTURE
Volume 21, Issue 4, Pages 550-559Publisher
CELL PRESS
DOI: 10.1016/j.str.2013.01.014
Keywords
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Funding
- German Research Foundation [SFB958/A12]
- International Human Frontier Science Program Organization
- EMBO Young Investigator Fellowship
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GTPases of immunity-associated proteins (GIMAPs) are regulators of lymphocyte survival and homeostasis. We previously determined the structural basis of GTP-dependent GIMAP2 scaffold formation on lipid droplets. To understand how its GTP hydrolysis is activated, we screened for other GIMAPs on lipid droplets and identified GIMAP7. In contrast to GIMAP2, GIMAP7 displayed dimerization-stimulated GTP hydrolysis. The crystal structure of GTP-bound GIMAP7 showed a homodimer that assembled via the G domains, with the helical extensions protruding in opposite directions. We identified a catalytic arginine that is supplied to the opposing monomer to stimulate GTP hydrolysis. GIMAP7 also stimulated GTP hydrolysis by GIMAP2 via an analogous mechanism. Finally, we found GIMAP2 and GIMAP7 expression differentially regulated in several human T cell lymphoma lines. Our findings suggest that GTPase activity in the GIMAP family is controlled by homo-and heterodimerization. This may have implications for the differential roles of some GIMAPs in lymphocyte survival.
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